PrimePCR™ ddPCR™ Copy Number Assay: IGF2, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   HEX; 60nt amplicon; crosses exon-intron junction; HEX version of IGF2 (dHsaCP2506278)

 
Fluorophore:   HEX
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP2506676
Chromosome Location:   hg1911:2156539-2156661question
Amplicon Length:   60
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

This gene encodes a member of the insulin family of polypeptide growth factors which are involved in development and growth. It is an imprinted gene expressed only from the paternal allele and epigenetic changes at this locus are associated with Wilms tumour Beckwith-Wiedemann syndrome rhabdomyosarcoma and Silver-Russell syndrome. A read-through INS-IGF2 gene exists whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2010]

Gene Symbol:   IGF2
Gene Name:   insulin-like growth factor 2 (somatomedin A)
Aliases:   C11orf43, FLJ22066, FLJ44734, IGF-II, PP9974
RefSeq:   NC_000011.9 NG_008849.1 NT_009237.18
Ensembl:   ENSG00000167244
Entrez:   3481
UniGene:   Hs.272259
Chromosome Mapping:   11p15.5

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

Number Description Download