This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
ddPCR™ probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
Info: HEX; 60nt amplicon; crosses exon-intron junction; HEX version of IGF2 (dHsaCP2506278)
This gene encodes a member of the insulin family of polypeptide growth factors which are involved in development and growth. It is an imprinted gene expressed only from the paternal allele and epigenetic changes at this locus are associated with Wilms tumour Beckwith-Wiedemann syndrome rhabdomyosarcoma and Silver-Russell syndrome. A read-through INS-IGF2 gene exists whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2010]
Products used to generate validation data:
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.
ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).