PrimePCR™ ddPCR™ Copy Number Assay: TOLLIP, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   HEX; 68nt amplicon; intronic; HEX version of TOLLIP (dHsaCP2500476)

 
Fluorophore:   HEX
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP2506667
Chromosome Location:   hg1911:1311798-1311920question
Amplicon Length:   68
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

This gene encodes a ubiquitin-binding protein that interacts with several Toll-like receptor (TLR) signaling cascade components. The encoded protein regulates inflammatory signaling and is involved in interleukin-1 receptor trafficking and in the turnover of IL1R-associated kinase. [provided by RefSeq Aug 2011]

Gene Symbol:   TOLLIP
Gene Name:   toll interacting protein
Aliases:   FLJ33531, IL-1RAcPIP
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000078902
Entrez:   54472
UniGene:   Hs.368527
Chromosome Mapping:   11p15.5

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

Number Description Download