PrimePCR™ ddPCR™ Copy Number Assay: PML, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   HEX; 58nt amplicon; exonic; HEX version of PML (dHsaCP2500446)

 
Fluorophore:   HEX
List Price:    €210.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP2506649
Chromosome Location:   hg1915:74290691-74290813question
Amplicon Length:   58
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains a RING a B-box type 1 and a B-box type 2 and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   PML
Gene Name:   promyelocytic leukemia
Aliases:   MYL, PP8675, RNF71, TRIM19
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000140464
Entrez:   5371
UniGene:   Hs.526464
Chromosome Mapping:   15q22

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

Number Description Download