PrimePCR™ ddPCR™ Copy Number Assay: IGF1R, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   HEX; 70nt amplicon; crosses exon-intron junction; HEX version of IGF1R (dHsaCP2500409)

 
Fluorophore:   HEX
List Price:    $254.00
Your Price:   Log In
 

Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP2506600
Chromosome Location:   hg1915:99477948-99478070question
Amplicon Length:   70
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. [provided by RefSeq Jul 2008]

Gene Symbol:   IGF1R
Gene Name:   insulin-like growth factor 1 receptor
Aliases:   CD221, IGFIR, IGFR, JTK13, MGC142170, MGC142172, MGC18216
RefSeq:   NC_000015.9 NG_009492.1 NT_010274.17
Ensembl:   ENSG00000140443
Entrez:   3480
UniGene:   Hs.643120
Chromosome Mapping:   15q26.3

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

Number Description Download