PrimePCR™ ddPCR™ Copy Number Assay: GSTO2, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   HEX; 70nt amplicon; crosses exon-intron junction; HEX version of GSTO2 (dHsaCP2500404)

 
Fluorophore:   HEX
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP2506597
Chromosome Location:   hg1910:106057378-106057500question
Amplicon Length:   70
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

The protein encoded by this gene is an omega class glutathione S-transferase (GST). GSTs are involved in the metabolism of xenobiotics and carcinogens. Four transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Jul 2010]

Gene Symbol:   GSTO2
Gene Name:   glutathione S-transferase omega 2
Aliases:   bA127L20.1
RefSeq:   NC_000010.10 NG_023362.1 NT_030059.13 NG_023363.1
Ensembl:   ENSG00000065621
Entrez:   119391
UniGene:   Hs.203634
Chromosome Mapping:   10q25.1

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

Number Description Download