PrimePCR™ ddPCR™ Copy Number Assay: GPC5, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   HEX; 67nt amplicon; exonic; HEX version of GPC5 (dHsaCP2500332)

 
Fluorophore:   HEX
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP2506512
Chromosome Location:   hg1913:92380787-92380909question
Amplicon Length:   67
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq Jul 2008]

Gene Symbol:   GPC5
Gene Name:   glypican 5
Aliases:   Not Available
RefSeq:   NC_000013.10 NG_009370.1 NT_009952.14
Ensembl:   ENSG00000179399
Entrez:   2262
UniGene:   Hs.655675
Chromosome Mapping:   13q32

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

Number Description Download