PrimePCR™ ddPCR™ Copy Number Assay: NOTCH1, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   HEX; 58nt amplicon; exonic; HEX version of NOTCH1 (dHsaCP1000509)

 
Fluorophore:   HEX
List Price:    $254.00
Your Price:   Log In
 

Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP2506354
Chromosome Location:   hg199:139407829-139407951question
Amplicon Length:   58
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats and an intracellular domain consisting of multiple different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia notch interaction with its cell-bound ligands (delta serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands and may play multiple roles during development. [provided by RefSeq Jul 2008]

Gene Symbol:   NOTCH1
Gene Name:   notch 1
Aliases:   TAN1, hN1
RefSeq:   NC_000009.11 NG_007458.1 NT_024000.16
Ensembl:   ENSG00000148400
Entrez:   4851
UniGene:   Hs.495473
Chromosome Mapping:   9q34.3

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

Number Description Download