PrimePCR™ ddPCR™ Copy Number Assay: CSF1R, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   HEX; 64nt amplicon; crosses exon-intron junction; HEX version of CSF1R (dHsaCP1000495)

 
Fluorophore:   HEX
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP2506338
Chromosome Location:   hg195:149449778-149449900question
Amplicon Length:   64
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

The protein encoded by this gene is the receptor for colony stimulating factor 1 a cytokine which controls the production differentiation and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. [provided by RefSeq Jul 2008]

Gene Symbol:   CSF1R
Gene Name:   colony stimulating factor 1 receptor
Aliases:   C-FMS, CD115, CSFR, FIM2, FMS
RefSeq:   NG_023367.1 NC_000005.9 NG_012303.1 NG_021389.1 NT_029289.11
Ensembl:   ENSG00000182578
Entrez:   1436
UniGene:   Hs.586219
Chromosome Mapping:   5q32

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

Number Description Download