PrimePCR™ ddPCR™ Copy Number Assay: CEBPA, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   HEX; 62nt amplicon; exonic; HEX version of CEBPA (dHsaCP1000494)

 
Fluorophore:   HEX
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP2506337
Chromosome Location:   hg1919:33792397-33792519question
Amplicon Length:   62
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin a protein that plays an important role in body weight homeostasis. Also the encoded protein can interact with CDK2 and CDK4 thereby inhibiting these kinases and causing growth arrest in cultured cells. [provided by RefSeq Jul 2008]

Gene Symbol:   CEBPA
Gene Name:   CCAAT/enhancer binding protein (C/EBP), alpha
Aliases:   C/EBP-alpha, CEBP
RefSeq:   NC_000019.9 NG_012022.1 NT_011109.16
Ensembl:   ENSG00000245848
Entrez:   1050
UniGene:   Hs.76171
Chromosome Mapping:   19q13.1

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

Number Description Download