PrimePCR™ ddPCR™ Copy Number Assay: KIF21A, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Centromeric 12q Assay (12q12); FAM; 69nt amplicon; crosses exon-intron junction; FAM version of KIF21A (dHsaCP2506719)

 
Fluorophore:   FAM
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP2506306
Chromosome Location:   hg1912:39740222-39740344question
Amplicon Length:   69
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

This gene encodes a member of the KIF4 subfamily of kinesin-like motor proteins. The encoded protein is characterized by an N-terminal motor domain a coiled-coil stalk domain and a C-terminal WD-40 repeat domain. This protein may be involved in microtubule dependent transport. Mutations in this gene are the cause of congenital fibrosis of extraocular muscles-1. Alternate splicing results in multiple transcript variants.[provided by RefSeq Mar 2010]

Gene Symbol:   KIF21A
Gene Name:   kinesin family member 21A
Aliases:   CFEOM1, DKFZp779C159, FEOM1, FEOM3A, FLJ20052, KIAA1708
RefSeq:   NC_000012.11 NT_029419.12 NG_017067.1
Ensembl:   ENSG00000139116
Entrez:   55605
UniGene:   Hs.374201
Chromosome Mapping:   12q12

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

Number Description Download