PrimePCR™ ddPCR™ Copy Number Assay: PIK3CA, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   FAM; 79nt amplicon; exonic; FAM version of PIK3CA Exon 20 (dHsaCP2506262)

 
Fluorophore:   FAM
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP2506260
Chromosome Location:   hg193:178951888-178952010question
Amplicon Length:   79
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit which uses ATP to phosphorylate PtdIns PtdIns4P and PtdIns(45)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. [provided by RefSeq Jul 2008]

Gene Symbol:   PIK3CA
Gene Name:   phosphoinositide-3-kinase, catalytic, alpha polypeptide
Aliases:   MGC142161, MGC142163, PI3K, p110-alpha
RefSeq:   NC_000003.11 NG_012113.1 NT_005612.16
Ensembl:   ENSG00000121879
Entrez:   5290
UniGene:   Hs.732403
Chromosome Mapping:   3q26.3

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

Number Description Download