PrimePCR™ ddPCR™ Copy Number Assay: MS4A1, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   FAM; 66nt amplicon; exonic; FAM version of MS4A1 (dHsaCP2506662)

 
Fluorophore:   FAM
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP2500429
Chromosome Location:   hg1911:60223291-60223413question
Amplicon Length:   66
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12 among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq Jul 2008]

Gene Symbol:   MS4A1
Gene Name:   membrane-spanning 4-domains, subfamily A, member 1
Aliases:   B1, Bp35, CD20, CVID5, LEU-16, MGC3969, MS4A2, S7
RefSeq:   NC_000011.9 NT_167190.1 NG_023388.1
Ensembl:   ENSG00000156738
Entrez:   931
UniGene:   Hs.712553
Chromosome Mapping:   11q12

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

Number Description Download