PrimePCR™ ddPCR™ Copy Number Assay: CDKN2C, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   FAM; 66nt amplicon; crosses exon-intron junction; FAM version of CDKN2C (dHsaCP2506500)

 
Fluorophore:   FAM
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP1000589
Chromosome Location:   1:51436088-51436210question
Amplicon Length:   66
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to interact with CDK4 or CDK6 and prevent the activation of the CDK kinases thus function as a cell growth regulator that controls cell cycle G1 progression. Ectopic expression of this gene was shown to suppress the growth of human cells in a manner that appears to correlate with the presence of a wild-type RB1 function. Studies in the knockout mice suggested the roles of this gene in regulating spermatogenesis as well as in suppressing tumorigenesis. Two alternatively spliced transcript variants of this gene which encode an identical protein have been reported. [provided by RefSeq Jul 2008]

Gene Symbol:   CDKN2C
Gene Name:   cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4)
Aliases:   INK4C, p18, p18-INK4C
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000123080
Entrez:   1031
UniGene:   Hs.525324
Chromosome Mapping:   1p32

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX200
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

Number Description Download