PrimePCR™ ddPCR™ Copy Number Assay: CTSD, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   FAM: 65nt amplicon; exonic

 
Fluorophore:   FAM
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP1000439
Chromosome Location:   11:1782601-1782723question
Amplicon Length:   65
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains both produced from a single protein precursor. This proteinase which is a member of the peptidase C1 family has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases including breast cancer and possibly Alzheimer disease. [provided by RefSeq Jul 2008]

Gene Symbol:   CTSD
Gene Name:   cathepsin D
Aliases:   CLN10, CPSD, MGC2311
RefSeq:   NC_000011.9 NG_008655.1 NT_009237.18
Ensembl:   ENSG00000117984
Entrez:   1509
UniGene:   Hs.654447
Chromosome Mapping:   11p15.5

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX100
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

Number Description Download