PrimePCR™ ddPCR™ Copy Number Assay: CHRNA6, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   FAM: 75nt amplicon; intronic

 
Fluorophore:   FAM
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP1000211
Chromosome Location:   8:42620436-42620558question
Amplicon Length:   75
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

This gene encodes an alpha subunit of neuronal nicotinic acetylcholine receptors. These receptors consist of five subunits and function as ion channels involved in neurotransmission. The encoded protein is a subunit of neuronal nicotinic acetylcholine receptors that mediate dopaminergic neurotransmission and are activated by acetylcholine and exogenous nicotine. Alternatively spliced transcript variants have been observed for this gene. Single nucleotide polymorphisms in this gene have been associated with both nicotine and alcohol dependence. [provided by RefSeq Dec 2010]

Gene Symbol:   CHRNA6
Gene Name:   cholinergic receptor, nicotinic, alpha 6
Aliases:   CHNRA6
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000147434
Entrez:   8973
UniGene:   Hs.103128
Chromosome Mapping:   8p11.21

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX100
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

Number Description Download