PrimePCR™ ddPCR™ Copy Number Assay: CHRNB2, Human

PrimePCR

ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   FAM: 65nt amplicon; exonic

 
Fluorophore:   FAM
List Price:    $254.00
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Assay Information

Technology:   ddPCR
Assay Type:   Probe
Application:   Copy Number Variation
Unique Assay ID:   dHsaCP1000138
Chromosome Location:   1:154548298-154548420question
Amplicon Length:   65
Reference Assays:   , , ,
Restriction Enzymes:   HaeIII

Gene Information

Neuronal acetylcholine receptors are homo- or heteropentameric complexes composed of homologous alpha and beta subunits. They belong to a superfamily of ligand-gated ion channels which allow the flow of sodium and potassium across the plasma membrane in response to ligands such as acetylcholine and nicotine. This gene encodes one of several beta subunits. Mutations in this gene are associated with autosomal dominant nocturnal frontal lobe epilepsy. [provided by RefSeq Jul 2008]

Gene Symbol:   CHRNB2
Gene Name:   cholinergic receptor, nicotinic, beta 2 (neuronal)
Aliases:   EFNL3, nAChRB2
RefSeq:   NC_000001.10 NG_008027.1 NT_004487.19
Ensembl:   ENSG00000160716
Entrez:   1141
UniGene:   Hs.2306
Chromosome Mapping:   1q21.3

  Download Validation Data (.pdf)


Products used to generate validation data:

ddPCR Instrument QX100
ddPCR Supermix ddPCR Supermix for Probes (no dUTP)
Experimental Sample NA18916,AG09394

Copy Number
Copy number analysis of two samples, using the EIF2C1 reference assay. Technical replicates are shown.

Copy Number Text

ddPCR Amplitude Scatter Plot
Single-well data of target assay (FAM, Channel 1) duplexed to EIF2C1 reference assay (HEX, Channel 2).

ddPCR Amplitude Scatter Plot Text

Number Description Download