GS-900™ calibrated densitometer and Image Lab™ software

How do I determine the correct filter to use when selecting a color for acquisition on the GS-900 densitometer?

If the stain is predominantly one color, the most effective way to use a densitometer is to scan the stain in its complementary color. For example, scan Coomassie Blue–stained samples using the complementary red filter. If you scan Coomassie Blue–stained samples with the blue filter, no data will be captured because the filter does not absorb blue.

Below is a partial list of stains to use with each of the three color filters:

  • Red filter (595–750 nm): Coomassie G-250 stain, BCIP/NBT, Fast Green FCF, methylene blue
  • Green filter (520–570 nm): Coomassie R-250 stain, Basic Fuchsin, BCIP/NBT, DAB horseradish peroxidase substrates, 4CN horseradish peroxidase, colloidal gold, Amido Black, silver stain, zinc stain, copper stain, photographic negatives and positives, gray X-ray film
  • Blue filter (400–530 nm): Crocein scarlet, blue X-ray film

Note: The red, green, and blue filters can be used in combination; two filters can be selected simultaneously.

For more information, consult the product literature on found on the GS-900 system product page.

Please contact technical support for further assistance with dyes not listed in the manual.