
Real-Time PCR Supermixes & Kits
qPCR Supermixes
Bio-Rad's supermixes can make any qPCR experiment easier, faster, and more effective. Our real-time PCR supermixes are designed for:
- Any instrument — universal reference dye is compatible with all qPCR platforms
- Any chemistry — supermixes for SYBR Green or probe-based detection chemistry
- Any conditions — our patented Sso7d fusion polymerase* guarantees superior qPCR performance under varying and/or challenging conditions
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![]() SsoAdvanced Universal SYBR Green Supermix |
![]() SsoAdvanced Universal Probes Supermix |
![]() SsoAdvanced Universal Inhibitor-Tolerant Supermix |
![]() iTaq Universal SYBR Green Supermix |
![]() iTaq Universal Probes Supermix |
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Features |
Robust performance with demanding templates and under challenging conditions |
Optimized for crude samples containing PCR inhibitors |
Advanced formulation for reliable, reproducible gene expression analysis on any qPCR instrument |
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Detection chemistry | SYBR Green | Probes | SYBR Green | SYBR Green | Probes |
Sso7d fusion polymerase | Yes | Yes | Yes | No | No |
Sensitive detection of low-abundance targets | ![]() |
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Efficient amplification of difficult targets | ![]() |
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Specific amplification of targets | ![]() |
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Robust performance under varying PCR conditions** | ![]() |
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Tolerance to PCR inhibitors | ![]() |
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Cycling protocol compatibility | Both standard and fast | ||||
Instrument compatibility | Any instrument | ||||
Documents |
** Primer concentrations, annealing temperatures, salt concentrations, and/or cycling protocol (standard or fast)

CFX Loyalty Program
Earn a free one-year service contract on your CFX System with purchase of Reagents
iQ & SsoFast PCR Supermixes
iQ and SsoFast PCR Supermixes have been supporting researchers performing basic real-time PCR analysis for years. If you have not yet used them, we recommend trying our new SsoAdvanced Universal SYBR Green and SsoAdvanced Universal Probes Supermixes as superior-performance alternatives for use on any real-time PCR instrument.
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Supermixes for Dye-Based qPCR Assays
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Supermixes for Probe-Based qPCR Assays
Resources

Understanding Real-Time PCR Supermixes
This interactive tutorial provides an introduction to the chemistry behind real-time PCR reactions and supermixes as well as a protocol for comparing different reagents.
Applications & Technologies
qPCR/Real-Time PCR Reagents
Guidelines for selecting the appropriate reagents for your real-time PCR-based application
Real-Time PCR Data Analysis
An overview of our CFX Manager™ Real-Time PCR Analysis Software features, including high resolution melt (HRM) analysis capabilities