Nucleic Acid Electrophoresis and Blotting
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|Nucleic Acid Separation Equipment||Imaging and Analysis Equipment||Gels and Standards||Buffers and Reagents||Stains and Tracking Dyes||Resources||Applications and Technologies|
Bio-Rad’s selection of electrophoresis and blotting instruments for nucleic acids includes submerged horizontal and vertical cells in various sizes, PFGE systems, and solutions for sequencing and mutation detection.
Nucleic Acid Electrophoresis and Blotting Equipment
Our Sub-Cell family of submerged horizontal electrophoresis cells enable versatile, multiple-sample, and rapid-screening DNA applications on precast or handcast gels in a variety of different gel sizes.
Electrophoresis cells and precast polyacrylamide gels are offered for high-resolution separation of nucleic acids in mini and midi formats. The gels are available in different sizes and buffer formulations.
Three CHEF systems that incorporate different technologies for optimal resolution in various size ranges, such as PACE, FIGE, and AFIGE are offered. Agarose, reagents, standards, and kits for PFGE are available.
Vacuum blotter, buffers, membranes, and equipment are offered for blotting DNA or RNA from an agarose gel onto membranes. Membranes include nitrocellulose, Zeta-Probe, Zeta-Probe GT, and C/P Lift membranes.
From basic to high-voltage capacity, our power supplies provide simple programming in a compact, stackable case.
The DCode universal mutation detection system enables mutation detection by various electrophoretic techniques.
Imaging and Analysis Equipment
Detect, image and quantify DNA/RNA gels stained with ethidium bromide, SYBR Green I, SYBR Safe Dye, Coumarin Stain, and Fast Blast DNA Stain.
UView 6x Loading Dye plus Mini Transilluminator includes dual-action DNA stain and loading dye, and personal UV transilluminator for visualizing agarose gel.
Gels and Standards
Choose from a variety of Precast ReadyAgarose gels in mini, wide, or 96-well DNA electrophoresis gels in 1% or 3% agarose, TBE or TAE buffer, with or without ethidium bromide formats.
Mini-PROTEAN TBE precast gels are suitable for electrophoresis of nucleic acids from 50 to 2,000 base pairs; they are ideal for analysis of the purity of PCR products, standard dsDNA analysis, and RNase protection assays.
Mini-PROTEAN TBE-Urea precast gels maintain denaturing conditions for analysis of single-stranded DNA and RNA. Nucleic acids between 60 and 200 bases are resolved as sharp, distinct bands.
TBE gels are suitable for electrophoresis of nucleic acids from 50 to 2,000 base pairs; they are ideal for evaluation of the purity of PCR products and standard DNA analyses.
TBE-urea gels maintain denaturing conditions for analysis of single-stranded DNA and RNA between 60 and 200 bases. Designed for the traditional 13.3 x 8.7 cm mini vertical format.
Nucleic acid standards include molecular rulers with evenly spaced banding patterns (DNA ladders), EZ Load standards premixed with loading buffer, and specialty products, such as pulsed field standards.
Buffers and Reagents
Nucleic acid electrophoresis buffers and reagents are available as individual reagents or as premixed buffers. Products include agarose powders and buffers for sample loading, gel casting, and electrophoresis.
Stains and Tracking Dyes
Stains and tracking dyes are offered for detecting nucleic acids in gels and monitoring electrophoresis runs. Silver stains and fluorescent gel stains are provided to detect nanogram quantities of nucleic acids.
Our self-help troubleshooting guide covers solutions to many common and not-so-common western blotting issues and helps your blots look their best.
The 2-D Doctor is a self-help guide developed by Bio-Rad that enables you to identify and troubleshoot your 2-D gel issues.
Applications and Technologies
Gel boxes, running buffers, available agarose types, as well as factors affecting resolution of DNA fragments, to help with agarose gel electrophoresis of DNA.
How can large DNA fragments be separated using PFGE? How to prepare plugs, optimize sample resolution, analyze images, and troubleshoot PFGE gels? Explore.
Comparison of parameters, sensitivity, and data analysis using Bio-Rad imaging systems and software to determine which imaging system is ideal for your application.
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