Digital PCR

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Digital PCR is a breakthrough technology that provides ultrasensitive and absolute nucleic acid quantification. It is particularly useful for low-abundance targets, targets in complex backgrounds, allelic variants (SNPs), and for monitoring subtle changes in target levels that cannot be detected with real-time PCR.

Digital PCR Assays and Kits

Digital PCR

Bio-Rad offers a comprehensive portfolio of Digital PCR Assays and Kits for numerous applications including Mutation Detection, Copy Number Determination, Genome Edit Detection, Gene Expression, Expert Design Assays, Residual DNA Quantification and Library Quantification.

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Digital PCR Systems, Software, and Consumables

Droplet Digital PCR Systems

Droplet Digital PCR Systems

Bio-Rad’s Droplet Digital PCR Systems provide ultrasensitive and absolute nucleic acid quantification.

QX ONE Droplet Digital PCR (ddPCR) System
A high throughput multiplexed and integrated droplet digital PCR system

QX200 AutoDG Droplet Digital PCR System
For high-throughput analysis

QX200 Droplet Digital PCR (ddPCR) System
For analyzing up to 96 samples

 
QX Software for Digital PCR Systems

QX Software for Digital PCR Systems

QX Software is a family of powerful software packages for acquisition and analysis of your Droplet Digital PCR (ddPCR) data. When connected to a ddPCR System, QX Software provides all necessary functionality to create, run, and analyze experiments on your samples.

Learn More About QX Software for Digital PCR Systems »

 

Digital PCR Consumables and Reagents

Purchase supermixes, droplet generation oil, cartridges, gaskets, plates & seals, plus pipette tips, and more, designed for Droplet Digital PCR Systems.

ddPCR Consumables

QXDx Products for In Vitro Diagnostics (IVD)

Droplet Digital PCR Systems

QXDx AutoDG ddPCR System

IVD Consumables, Assays, and Kits

Droplet Digital PCR Technology

Based on water-emulsion droplet technology, Droplet Digital PCR fractionates a DNA sample in 20,000 droplets. PCR amplification of the template subsequently occurs in each individual droplet, and counting the positive droplets gives precise, absolute target quantification.

Read more about Droplet Digital PCR technology or learn how to transition your assay from qPCR to ddPCR.

  • DNA sample with target sequence is partitioned into droplets

    DNA sample with target sequence is partitioned into droplets

  • Target and background DNA are randomly distributed among 20,000 droplets

    Target and background DNA are randomly distributed among 20,000 droplets

  • Target sequence is amplified by end-point PCR in each droplet

    Target sequence is amplified by end-point PCR in each droplet

  • Positive droplets are counted to give a precise quantitation of target sequences in sample

    Positive droplets are counted to give precise quantification of target sequences in sample

Benefits of Droplet Digital PCR

Absolute quantification — provides an absolute count of target DNA copies per input sample without the need for standard curves

Highest precision and sensitivity — enriched template concentration in target-positive droplets allows higher precision and sensitivity than real-time PCR

High throughput — simple and easy-to-use workflow with 96-sample throughput does not require multiple dilution steps

Removal of PCR efficiency bias — error rates are reduced by removing the reliance on amplification efficiency of qPCR

Explore Absolute Quantification with Droplet Digital PCR, download the White Paper on Molecular Counting.

Applications of Droplet Digital PCR

Bio-Rad Droplet Digital PCR Publications

Droplet Digital PCR Publications

Browse the growing list of cited peer-reviewed journal articles in Droplet Digital PCR research featuring Bio-Rad products.

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