INTRODUCINGThe Reliance Select cDNA Synthesis Kit
Overcome challenging RT-qPCR samples in a single assay.
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Reverse transcription is a crucial step for gene expression studies, sequencing, cloning, and other applications. Optimization of this step is a priority to researchers to ensure the best results downstream. We offer a variety of cDNA synthesis kits to meet researchers' needs, including high fidelity or dealing with challenging samples, difficult targets, and rare or long transcripts.
cDNA Synthesis Kits
Reliance Select cDNA Synthesis Kit
The Reliance Select Kit enables researchers to synthesize cDNA with the highest level of sensitivity and robustness by utilizing the unique properties of its novel chimeric reverse transcriptase.
- Detection of very rare transcripts
- Processing of of samples in complex matrices, such as FFPE samples
- Overcoming difficult inhibitors
- Challenging targets with secondary structures and high GC concentration
iScript cDNA Synthesis Kits
iScript Reverse Transcription Reagents feature an RNase H+ MMLV reverse transcriptase plus an RNase inhibitor for reliable yields. Choose the best cDNA Synthesis kit for your application:
- One-tube supermix
- gDNA clearance
- High RNA input
- High fidelity
How can Bio-Rad help with your genomics research?
PCR/qPCR Reagents Selector
Not sure which reagents are the best for your PCR or qPCR experiment? Use our interactive selection tool to find the right products for your needs. Filter by application, technique, detection chemistry, or product-specific features to easily compare reagents.
Understanding Reverse Transcription
This interactive tutorial provides an introduction to reverse transcription basics, an explanation of how different reagents work, and a protocol for comparing the results with different reverse transcription reagents.
Reagent Comparison Guide for Real-Time PCR
(PDF 3 MB)
Amplification Reagents and Plastics Brochure
(PDF 6.1 MB)
iScript Reverse Transcription Kits Flier
(PDF 208 KB)
Reliance Select cDNA Synthesis Kit: Overcome Challenging Samples
Introducing Reliance Select cDNA Synthesis Kit. Don't let difficult samples stand between you and your data. The Reliance Select cDNA Synthesis Kit was designed for samples that contain inhibitors, secondary structure, and degraded RNA, including formalin-fixed paraffin-embedded (FFPE) samples.
Applications & Technologies
qPCR/Real-Time PCR Reagents
Guidelines for selecting the appropriate reagents for your real-time PCR-based application
Gene Expression Analysis
An introduction to gene regulation mechanisms, methods for gene expression profiling, and quantitation techniques including real-time PCR
lncRNA RT-qPCR Workflow
An innovative workflow that enables highly sensitive detection of lncRNAs and helps to overcome challenges associated with lncRNA expression analysis
Real-Time PCR Doctor
Having trouble with real-time PCR experiments? The Real-Time PCR Doctor can help you diagnose and correct common qPCR problems
Real-Time PCR Supermixes and Kits
PCR and real-time PCR reagents with advanced enzyme engineering and formulations optimized for a wide variety of templates and target sequences
One-Step RT-qPCR from RNA or Cells
For robust reverse transcription and SYBR Green or probe-based qPCR in one step on any instrument
Real-Time PCR Detection Systems
An automation-compatible, gradient-enabled thermal cycler with interchangeable optical reaction modules for singleplex or multiplex real-time PCR
Cell Lysis RT-qPCR Kits
Complete and fast solution for one- or two-step qPCR reactions directly from cell lysates with no RNA purification step
Thermal Cyclers for PCR
Personal units and flexible modular thermal cyclers provide low- to high-throughput capabilities for all your PCR amplification needs
Ready-to-use supermix for unbiased, target-specific preamplification of cDNA or gDNA using up to 100 qPCR assays
PCR Plastic Consumables
A large selection of thin-wall polypropylene PCR tubes, PCR plates, seals, and accessories precisely manufactured for various PCR applications