Science of Opioid Dependence Kit

Science of Opioid Dependence Kit

Have your students explore the environmental and genetic links to opioid dependence as they design a human genetic research study.

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Overview

DNA gels

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opioid dependence kit

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The Science of Opioid Dependence Kit puts your students in the roles of doctor and researcher as they explore potential genetic links to opioid dependence. In this kit, your students will outline a human genetic research study; they will conduct gel electrophoresis on pre-amplified PCR patient DNA samples to determine whether a mutation in a human dopamine receptor gene influences opioid dependence. Your students will also consider environmental factors that contribute to dependence as a phenotype.

Features and Benefits

  • Curriculum connections to genetics, neurobiology, DNA structure, PCR, and statistical analysis
  • Pre-amplified PCR DNA samples to teach PCR without a thermal cycler
  • Five activities with optional timelines and branch points
  • Reagents and consumables for 8 lab stations (32 students)

Students will

  • Explore the links between opioid dependence, genetics, the neurobiology of reward pathways, and environment factors
  • Design a human genetic research study and experiments
  • Analyze pre-amplified PCR patient DNA samples by agarose gel electrophoresis
  • Review class and published allele frequency data with optional chi-square analysis

Product Comparison

 
17005316EDU
Science of Opioid
Dependence Kit
(17005316EDU)
170052973EDU_PD_Detail
Science of Opioid Dependence Kit plus Fast Blast Electrophoresis Reagents
(17005297EDU)
17005313EDU_PD_Detail
Science of Opioid Dependence Kit plus UView Electrophoresis Reagents
(17005313EDU)
DNA Samples
 
 
 
PCR Molecular Weight Ruler
 
 
 
Orange G Loading Dye, 5x
 
 
 
1.5 ml Microcentrifuge tubes
 
 
 
Fast Blast DNA Stain, 500x  
 
 
Electrophoresis Buffer, TAE, 50x  
 
 
Certified Molecular Biology Agarose  
 
 
UView 6x Loading Dye and Stain    
 

Ordering

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17005316EDU
Electrophoresis and genetics kit, includes DNA samples, molecular weight ruler, loading dye, and microcentrifuge tubes for 32 students; educational use only
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17005297EDU_SKU_TN.jpg
17005297EDU
Electrophoresis and genetics kit, includes DNA samples, molecular weight ruler, loading dye, Fast Blast DNA stain, agarose, TAE buffer, and microcentrifuge tubes for 32 students; educational use only
Image
17005313EDU_SKU_TN.jpg
17005313EDU
Electrophoresis and genetics kit, includes DNA samples, molecular weight ruler, UView 6x Loading Dye and Stain, agarose, TAE buffer, and microcentrifuge tubes for 32 students; educational use only

Refills

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1660450EDU
Agarose gel preparation kit,makes 16–48 mini gels, includes agarose powder, Fast Blast DNA Stain, buffer; education use only
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1660462EDU
Agarose gel preparation kit,makes 16–48 mini gels, includes agarose powder, UView 6x Loading Dye and Stain, buffer; education use only
Image
uview-6x-loading-dye-166-5111edu-thumb.jpg
1665112EDU
1,000 µl, 6x dual-action nontoxic fluorescent nucleic acid stain and loading dye; education use only
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1613015EDU

8-well precast gel, TAE, 1.0% agarose, 7 x 10 cm; education use only

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1613116EDU
5 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases; education use only
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161-3100EDU_tn.jpg
1613100EDU
25 g, general purpose agarose for nucleic acid electrophoresis, certified free of inhibitors, DNases, RNases; education use only
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223-9480EDU_tn.jpg
2239480EDU
Pkg of 500, graduated micro test tube with pierceable top, 1.5 ml, autoclavable, natural; education use only

Documents

Number Description Options
10000113752
Science of Opioid Dependence Kit Instructor's Guide
10000113753
Science of Opioid Dependence Kit Student Guide
7176
Opioid Dependence Infographic