Overview
Run your DNA gels in 10 minutes
Find Out HowSee the Science of Opioid Dependence Kit in action
View WebinarThe Science of Opioid Dependence Kit puts your students in the roles of doctor and researcher as they explore potential genetic links to opioid dependence. In this kit, your students will outline a human genetic research study; they will conduct gel electrophoresis on pre-amplified PCR patient DNA samples to determine whether a mutation in a human dopamine receptor gene influences opioid dependence. Your students will also consider environmental factors that contribute to dependence as a phenotype.
Features and Benefits
- Curriculum connections to genetics, neurobiology, DNA structure, PCR, and statistical analysis
- Pre-amplified PCR DNA samples to teach PCR without a thermal cycler
- Five activities with optional timelines and branch points
- Reagents and consumables for 8 lab stations (32 students)
Students will
- Explore the links between opioid dependence, genetics, the neurobiology of reward pathways, and environment factors
- Design a human genetic research study and experiments
- Analyze pre-amplified PCR patient DNA samples by agarose gel electrophoresis
- Review class and published allele frequency data with optional chi-square analysis
Product Comparison
Science of Opioid Dependence Kit (17005316EDU) |
Science of Opioid Dependence Kit plus Fast Blast Electrophoresis Reagents (17005297EDU) |
Science of Opioid Dependence Kit plus UView Electrophoresis Reagents (17005313EDU) |
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DNA Samples |
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PCR Molecular Weight Ruler |
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Orange G Loading Dye, 5x |
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1.5 ml Microcentrifuge tubes |
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Fast Blast DNA Stain, 500x |
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Electrophoresis Buffer, TAE, 50x |
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Certified Molecular Biology Agarose |
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UView 6x Loading Dye and Stain |
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Refills
100 ml, TAE nucleic acid electrophoresis buffer solution, pH 8.0; education use only
8-well precast gel, TAE, 1.0% agarose, 7 x 10 cm; education use only