Cloning and Sequencing Explorer Series

Overview

Flowchart

Cloning and Sequencing Explorer Series Flowchart

Lab Preparation Checklist

Cloning and Sequencing Explorer Series Contents

Lab contains sufficient materials for 12 workstations (2–4 students per workstation).
Nucleic Acid Extraction Module	1
GAPDH PCR Module	1
Small UView™ DNA Electrophoresis Reagents Module	1
PCR Kleen™ Spin Purification Module	1
Ligation and Transformation Module	1
Microbial Culturing Module	1
Aurum™ Plasmid Mini Purification Module	1
Sequencing and Bioinformatics Module	1
1.5 ml EZ Micro™ test tubes, pkg. of 500	1
Required Accessories Not Included in Kit:
Plant samples
95–100% lab grade ethanol
Horizontal gel electrophoresis chambers
Thermal cycler
Adjustable micropipets
Pipet tips, aerosol barrier
Incubation oven
Water bath and dry bath
Microcentrifuges capable of greater than 12,000 x g
Power supplies
Gel documentation system
Shaking water bath or shaking incubator
Recommended (Optional) Accessories:
Vortexer
Microwave oven/autoclave
Gel documentation system
Spectrophotometer and cuvettes
Available Separately:
Nucleic Acid Extraction Module (1665005EDU)	1
GAPDH PCR Module (1665010EDU)	1
Small UView DNA Electrophoresis Reagents Module (1660462EDU)	1
PCR Kleen Spin Purification Module (7326300EDU)	1
Ligation and Transformation Module (1665015EDU)	1
Microbial Culturing Module (1665020EDU)	1
Aurum Plasmid Mini Purification Module (7326400EDU)	1
Sequencing and Bioinformatics Module (1665025EDU)	1
1.5 ml EZ Micro™ test tubes, pkg. of 500 (2239480EDU)	1

Planning Guide

Cloning and Sequencing Explorer Planning Guide

Overview

The intention of this planning guide is to provide an overview of the entire student research project including details of timelines, materials included, and equipment requirements so that instructors may prepare their curriculum and estimate budgetary requirements in advance. These are estimates based on current information available, and may change.

To read more click here

Conventional PCR Animation

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The Cloning and Sequencing Explorer Series gives your students the tools to perform novel research and contribute to the world's body of scientific knowledge. Over 8 modules, students use classic and cutting-edge molecular biology techniques to clone and sequence a portion of the GAPC gene from a plant of their choice. GAPC is a housekeeping gene in the glyeraldehyde-3-phosphate dehydrogenase (GAPDH) family and is well conserved among plants.

The Cloning and Sequencing Explorer Series guides students through the following experimental workflow steps:

Extract genomic DNA from plants
Amplify a GAPDH gene using PCR and analyze using electrophoresis
Purify the PCR product
Ligate the PCR product into a plasmid
Transform the ligated plasmid into bacteria
Grow minipreps of the transformed bacteria
Isolate plasmid DNA and analyze by restriction digestion
Sequence plasmid DNA (sequencing service not included)
Analyze GAPDH gene sequence using bioinformatics

Features and Benefits

Designed by undergraduate and community college educators, this course was specifically developed to engage your students in authentic research, while ensuring success for both you and them. Tailored experimental controls allow your students to:

Continue the experiment to its conclusion even with negative results
Make mistakes, learn from them, and still complete every step of the workflow
Experience firsthand the satisfaction of completing a successful research project
Apply molecular biology skills and techniques to complete a real-world research project

The Cloning and Sequencing Explorer Series allows students to apply molecular biology skills and techniques to complete a real-world research project, potentially encouraging them to pursue a career in research.

Now with Geneious Prime!

The Cloning and Sequencing Explorer Series now includes a 4-month subscription to Geneious Prime Bioinformatics Software to support your students' scientific discovery.

For more information, visit the Sequencing and Bioinformatics Module product page.

More Information

6–8 week series of laboratory activities made up of eight modular kits
Provides sufficient materials for 12 student workstations, up to four students per workstation
Sequencing service not included.

Additional Resources

Cloning and Sequencing Explorer Series Instruction Manual
Example Chinese Broccoli sequence files

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Cloning and Sequencing Explorer Series

1665000EDU
Molecular biology study kit, includes 8 modules with lab activities from DNA extraction and cloning to computer-based sequence analysis, curriculum (sequencing service not included); education use only

0 1665000EDU /templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/166-5000EDU_0921121012345.xml 1665000EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jc99dsny 2017-11-14 07:07:14 true true internet/product_SKU jc99dsny templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/166-5000EDU_0921121012345.xml LSE 2019-05-04 11:13:22 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972631601 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 11:13:22 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/166-5000EDU_0921121012345.xml templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/166-5000EDU_0921121012345.xml 1665000edu-cloning-sequencing-explorer-series 1665000EDU Cloning and Sequencing Explorer Series Cloning and Sequencing Explorer Series Molecular biology study kit, includes 8 modules with lab activities from DNA extraction and cloning to computer-based sequence analysis, curriculum (sequencing service not included); education use only Molecular biology study kit, includes 8 modules with lab activities from DNA extraction and cloning to computer-based sequence analysis, curriculum; education use only Molecular biology study kit, includes 8 modules with lab activities from DNA extraction and cloning to computer-based sequence analysis, curriculum (sequencing service not included); education use only /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_thumb/global/166-5000EDU_tn.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_view/global/166-5000EDU_view.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_feature/global/166-5000EDU_feature.jpg preview ,VN RepEx 166-5000EDU Molecular biology study kit, includes 8 modules with lab activities from DNA extraction and cloning to computer-based sequence analysis, curriculum (sequencing service not included); education use only 1665000EDU, 1665000, cloning, sequencing, dna sequencing, gene sequencing, explorer, molecular biology, bioinformatics, lab, dna extraction, dna purification, education, classroom 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore N 0 <p>Use the Cloning and Sequencing Explorer series to give your students the tools to perform novel research and contribute to the world's body of scientific knowledge. Over 8 modules, students use classic and cutting-edge molecular biology techniques to clone and sequence a portion of the <em>GAPC</em> gene from a plant of their choice. <em>GAPC</em> is a housekeeping gene in the glyeraldehyde-3-phosphate dehydrogenase (GAPDH) family and is well conserved among plants.</p> <p>The Cloning and Sequencing Explorer Series guides students through the following experimental workflow steps:</p> <ol> <li>Extract genomic DNA from plants</li> <li>Amplify a <em>GAPDH</em> gene using PCR and analyze using electrophoresis</li> <li>Purify the PCR product</li> <li>Ligate the PCR product into a plasmid</li> <li>Transform the ligated plasmid into bacteria</li> <li>Grow minipreps of the transformed bacteria</li> <li>Isolate plasmid DNA and analyze by restriction digestion</li> <li>Sequence plasmid DNA (sequencing service not included)</li> <li>Analyze <em>GAPDH</em> gene sequence using bioinformatics</li> </ol> <h2 class="descHeader">Features and Benefits</h2> <ul> <li>6–8 week series of laboratory activities made up of eight modular kits</li> <li>Provides sufficient materials for 12 student workstations, up to four students per workstation</li> <li>Sequencing service not included</li> </ul> <p>For more information and resources, visit the <a href="/en-us/product/cloning-sequencing-explorer-series">Cloning and Sequencing Explorer Series</a> product page.</p> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/nucleic-acid-extraction-module">Nucleic Acid Extraction Module</a></li> <li><a href="/en-us/product/gapdh-pcr-module">GAPDH PCR Module</a></li> <li><a href="/en-us/product/electrophoresis-module">Electrophoresis Module</a></li> <li><a href="/en-us/product/pcr-kleen-spin-purification-module">PCR Kleen™ Spin Purification Module</a></li> <li><a href="/en-us/product/ligation-transformation-module">Ligation and Transformation Module</a></li> <li><a href="/en-us/product/microbial-culturing-module">Microbial Culturing Module</a></li> <li><a href="/en-us/product/aurum-plasmid-mini-purification-module">Aurum™ Plasmid Mini Purification Module</a></li> <li><a href="/en-us/product/sequencing-bioinformatics-module">Sequencing and Bioinformatics Module</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li><a href="/webroot/web/pdf/lse/literature/10000069187.pdf" target="_blank">Bio-Rad Explorer™ Cloning and Sequencing Explorer Series Curriculum Manual</a><br /> <a href="/webroot/web/pdf/lse/literature/Bulletin_6858.pdf" target="_blank"></a></li> </ul> /webroot/web/html/lse/products/SKUs/autopopulated 1665000EDU-desc.html /webroot/web/html/lse/products/SKUs/autopopulated/1665000EDU-desc.html 10000053330 templatedata/internet/documentation/data/LSE/Global/English/Literature/10000053330_1453418783_en_XX.xml false £1,846.00 false $ null true false true true false GBP true true true /prd/en/UK/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665000EDU&quantity= false false /prd/en/UK/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665000EDU&CartQuote=QT&quantity= 1711 true true false /prd/en/UK/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665000EDU&AddToDefaultWishList=true&quantity= 12161500 1846.00 1665000EDU

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(Discontinued) Nucleic Acid Extraction Module

1665005EDU
First module in Cloning and Sequencing Explorer Series classroom study kit, includes buffers, DTT, test tubes

This product is no longer available. Please contact technical support for more information on replacement product.

(Discontinued) GAPDH PCR Module

1665010EDU
Second module in Cloning and Sequencing Explorer Series classroom study kit, includes primers, PCR master mix, test tubes/holders, curriculum

This product is no longer available. Please contact technical support for more information on replacement product.

Small Ethidium Bromide DNA Electrophoresis Reagents Pack

1660451EDU
Agarose gel preparation kit,makes 16–48 mini gels, includes agarose powder, ethidium bromide, buffer; education use only

1660451EDU /templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Electrophoresis-Module/166-0451EDU_0921121012020.xml 5 1660451EDU 09/21/09 12:10 PM en_US true internet/product_SKU 2019-08-12 20:26:43 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Electrophoresis-Module/166-0451EDU_0921121012020.xml /default/main/bio-rad/WORKAREA/default jc99dsp5 2017-11-14 07:07:15 true jc99dsp5 templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Electrophoresis-Module/166-0451EDU_0921121012020.xml LSE N /default/main/bio-rad en 12/31/37 11:59 PM 1556969067279 2019-08-12 20:26:43 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Electrophoresis-Module/166-0451EDU_0921121012020.xml 1660451edu-small-ethidium-bromide-dna-electrophoresis-reagents-pack 1 1660451EDU Small Ethidium Bromide DNA electrophoresis reagents pack Small Ethidium Bromide DNA Electrophoresis Reagents Pack Agarose gel preparation kit,makes 16–48 mini gels, includes agarose powder, ethidium bromide, buffer; education use only Agarose gel preparation kit, makes forty-eight 1% or sixteen 3% 7 x 10 cm gels, includes agarose powder, ethidium bromide, buffer; education use only Agarose gel preparation kit,makes 16–48 mini gels, includes agarose powder, ethidium bromide, buffer; education use only /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_thumb/global/166-0451EDU_tn.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_view/global/166-0451EDU_view.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_feature/global/166-0451EDU_feature.jpg Small Ethidium Bromide DNA Electrophoresis Reagents Pack #166-0451EDU RepEx Small Ethidium Bromide DNA Electrophoresis Reagents Pack #166-0451EDU Agarose gel preparation kit, makes forty-eight 1% or sixteen 3% 7 x 10 cm gels, includes agarose powder, ethidium bromide, buffer; education use only 166-0451EDU, 1660451EDU, small, ethidium, bromide, dna, electrophoresis, reagents, pack, agarose, gel, preparation, science, education, products, classroom 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Cloning-and-Sequencing-Explore/Electrophoresis-Module N 0 <div class="corner-box corner-box-promo corner-box-promo-edu linkpointer"><img src="/webroot/web/images/lse/products/dna_analysis_kits/product_detail/global/dna-gels-th.jpg" border="0" alt="DNA gels" /> <p><strong>Run your DNA gels in 10 minutes!</strong></p> <a class="bttn-block no-target-style" href="/webroot/web/pdf/lse/literature/Bulletin_5396.pdf" target="_blank" rel="noopener noreferrer"><span class="bttn-orange">Find Out How</span></a></div> <p class="wraptxt">The Small Ethidium Bromide DNA Electrophoresis Reagents Pack makes 48 1% or 16 3% 7 x 10 cm gels. Materials include agarose powder, ethidium bromide, and buffer. This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated/ 166-0451EDU_0921121012020_en.html /webroot/web/html/lse/products/SKUs/autopopulated//166-0451EDU_0921121012020_en.html <b>Makes 48 1% or 16 3% 7 x 10 cm agarose gels</b> 1145   1 0 Agarose powder, 25 g 2455 1 1 0 Ethidium bromide, 10 ml 2703 1 1 0 Electrophoresis buffer, 50x TAE, 100 ml 2419 1 1 0 false £150.00 false $ null false false true true false GBP true false true /prd/en/UK/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660451EDU&quantity= false false /prd/en/UK/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660451EDU&CartQuote=QT&quantity= 1711 true true false /prd/en/UK/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660451EDU&AddToDefaultWishList=true&quantity= 41105300 150.00 1660451EDU

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Small Fast Blast DNA Electrophoresis Reagents Pack

1660450EDU
Agarose gel preparation kit,makes 16–48 mini gels, includes agarose powder, Fast Blast DNA Stain, buffer; education use only

1660450EDU /templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Electrophoresis-Module/166-0450EDU_0921121012040.xml 6 1660450EDU 09/21/09 12:10 PM en_US true internet/product_SKU 2019-08-12 20:32:36 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Electrophoresis-Module/166-0450EDU_0921121012040.xml /default/main/bio-rad/WORKAREA/default jc99dsp1 2017-11-14 07:07:15 true jc99dsp1 templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Electrophoresis-Module/166-0450EDU_0921121012040.xml LSE N /default/main/bio-rad en 12/31/37 11:59 PM 1556969067225 2019-08-12 20:32:36 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Electrophoresis-Module/166-0450EDU_0921121012040.xml 1660450edu-small-fast-blast-dna-electrophoresis-reagents-pack 1 1660450EDU Small Ethidium Bromide DNA electrophoresis reagents pack Small Fast Blast DNA Electrophoresis Reagents Pack Agarose gel preparation kit,makes 16–48 mini gels, includes agarose powder, Fast Blast DNA Stain, buffer; education use only Agarose gel preparation kit, makes forty-eight 1% or sixteen 3% 7 x 10 cm gels, includes agarose powder, ethidium bromide, buffer; education use only Agarose gel preparation kit,makes 16–48 mini gels, includes agarose powder, Fast Blast DNA Stain, buffer; education use only /webroot/web/images/lse/products/equipment_supplies/sku_thumb/global/166-0450EDU_tn.jpg /webroot/web/images/lse/products/equipment_supplies/sku_view/global/166-0450EDU_view.jpg /webroot/web/images/lse/products/equipment_supplies/sku_feature/global/166-0450EDU_feature.jpg Small Fast Blast™ DNA Electrophoresis Reagents Pack #166-0450EDU Tommy Alvaran Small Fast Blast™ DNA Electrophoresis Reagents Pack #166-0450EDU Agarose gel preparation kit, makes forty-eight 1% or sixteen 3% 7 x 10 cm gels, includes agarose powder, ethidium bromide, buffer; education use only 1660450, 1660450EDU, small, fast, blast, dna, electrophoresis, reagents, pack, agarose, gel, preparation, stain, buffer, science, education, products, classroom 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Cloning-and-Sequencing-Explore/Electrophoresis-Module N 0 <div class="corner-box corner-box-promo corner-box-promo-edu linkpointer"><img src="/webroot/web/images/lse/products/dna_analysis_kits/product_detail/global/dna-gels-th.jpg" border="0" alt="DNA gels" /> <p><strong>Run your DNA gels in 10 minutes!</strong></p> <a class="bttn-block no-target-style" href="/webroot/web/pdf/lse/literature/Bulletin_5396.pdf" target="_blank" rel="noopener noreferrer"><span class="bttn-orange">Find Out How</span></a></div> <p class="wraptxt">The Small Fast Blast DNA Electrophoresis Reagents Pack makes 48 1% or 16 3% 7 x 10 cm gels. Materials include agarose powder, Fast Blast DNA Stain, and buffer. This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated/ 166-0450EDU_0921121012040_en.html /webroot/web/html/lse/products/SKUs/autopopulated//166-0450EDU_0921121012040_en.html <b>Makes 48 1% or 16 3% 7 x 10 cm agarose gels</b> 1145   1 0 Agarose powder, 25 g 2455 1 1 0 Fast Blast™ DNA stain, 500x, 100 ml 2420 1 1 0 Electrophoresis buffer, 50x TAE, 200 ml 2702 1 1 0 false £112.00 false $ null true false true true false GBP true true true /prd/en/UK/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660450EDU&quantity= false false /prd/en/UK/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660450EDU&CartQuote=QT&quantity= 1717 false true false /prd/en/UK/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660450EDU&AddToDefaultWishList=true&quantity= 12000000 112.00 1660450EDU

List Price:		£112.00 Loading
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(Discontinued) Medium Fast Blast DNA Electrophoresis Reagent Pack

1660455EDU
Agarose gel preparation kit,makes 90–270 mini gels, includes agarose powder, Fast Blast DNA Stain, buffer; education use only

This product is no longer available. Please contact technical support for more information on replacement product.

(Discontinued) Large Fast Blast DNA Electrophoresis Reagent Pack

1660460EDU
Agarose gel preparation kit,makes 360–1,080 mini gels, includes agarose powder, Fast Blast DNA Stain, buffer; education use only

This product is no longer available. Please contact technical support for more information on replacement product.

(Discontinued) Medium Ethidium Bromide DNA Electrophoresis Reagents Pack

1660456EDU
Agarose gel preparation kit,makes 90–270 mini gels, includes agarose powder, ethidium bromide, buffer; education use only

This product is no longer available. Please contact technical support for more information on replacement product.

(Discontinued) Large Ethidium Bromide DNA Electrophoresis Reagents Pack

1660461EDU
Agarose gel preparation kit,makes 360–1,080 mini gels, includes agarose powder, ethidium bromide, buffer; education use only

This product is no longer available. Please contact technical support for more information on replacement product.

PCR Kleen™ Spin Purification Module

7326300EDU
Fourth module in Cloning and Sequencing Explorer Series classroom study kit, includes PCR Kleen Spin columns, test tubes, curriculum, for 12 workstations; education use only

7326300EDU /templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/732-6300EDU_0921121012431.xml 11 7326300EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jc99dsn5 2017-11-14 07:07:14 true true internet/product_SKU jc99dsn5 templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/732-6300EDU_0921121012431.xml LSE 2019-05-04 11:13:39 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972631937 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 11:13:39 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/732-6300EDU_0921121012431.xml templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/732-6300EDU_0921121012431.xml 7326300edu-pcr-kleen-spin-purification-module 7326300EDU PCR Kleen Spin Purification Module PCR Kleen™ Spin Purification Module Fourth module in Cloning and Sequencing Explorer Series classroom study kit, includes PCR Kleen Spin columns, test tubes, curriculum, for 12 workstations; education use only Fourth module in Cloning and Sequencing Explorer Series classroom study kit, includes PCR Kleen Spin columns, test tubes, curriculum, for 12 workstations; education use only Fourth module in Cloning and Sequencing Explorer Series classroom study kit, includes PCR Kleen Spin columns, test tubes, curriculum, for 12 workstations; education use only /webroot/web/images/lse/products/equipment_supplies/sku_thumb/global/732-6300EDU_tn.jpg /webroot/web/images/lse/products/equipment_supplies/sku_view/global/732-6300EDU_view.jpg /webroot/web/images/lse/products/equipment_supplies/sku_feature/global/732-6300EDU_feature.jpg PCR Kleen™ Spin Purification Module #732-6300EDU ,VN RepEx PCR Kleen™ Spin Purification Module #732-6300EDU Fourth module in Cloning and Sequencing Explorer Series classroom study kit, includes PCR Kleen Spin columns, test tubes, curriculum, for 12 workstations; education use only 7326300, 7326300EDU, pcr, kleen, spin, columns, dna, fragment, product, purification, clean-up, cleanup, science, education, products, classroom 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore N 0 <p>The fourth module in Cloning and Sequencing Explorer Series, the PCR Kleen™ Spin Purification Module uses size exclusion chromatography (SEC) to remove unincorpoated primers, nucleotides, salts, and enzymes from PCR products. The kit includes PCR Kleen spin columns, test tubes, and curriculum. Materials are sufficient for 12 workstations. This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated/ 732-6300EDU_0921121012431_en.html /webroot/web/html/lse/products/SKUs/autopopulated//732-6300EDU_0921121012431_en.html <b>Kit contains sufficient materials for 12 student workstations (2–4 students per workstation)</b> 1139   1 0 PCR Kleen™ spin columns 1953 25 1 0 Microcentrifuge tubes, 2.0 ml 2686 25 1 0 Microcentrifuge tubes, 1.5 ml 2428 25 1 0 Curriculum, including teacher's guide, student manual, and graphic quick guide 2398 1 1 0 false £110.00 false $ null true false true true false GBP true true true /prd/en/UK/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7326300EDU&quantity= false false /prd/en/UK/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7326300EDU&CartQuote=QT&quantity= 1711 false true false /prd/en/UK/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7326300EDU&AddToDefaultWishList=true&quantity= 41105500 110.00 7326300EDU

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(Discontinued) Ligation And Transformation Module

1665015EDU
Fifth module in Cloning and Sequencing Explorer Series classroom study kit, includes T4 ligase, cloning vector, growth medium, curriculum, and more, for 12 workstations; education use only

This product is no longer available. Please contact technical support for more information on replacement product.

(Discontinued) Microbial Culturing Module

1665020EDU
Sixth module in Cloning and Sequencing Explorer Series classroom study kit, includes ampicillin, petri dishes, E. coli strain HB101 K-12, curriculum, and more, for 12 workstations; education use only

This product is no longer available. Please contact technical support for more information on replacement product.

Aurum Plasmid Mini Purification Kit

7326400EDU
Plasmid purification kit contains plasmid-binding mini columns, capless collection tubes, reagents, for 100 preps

7326400EDU /templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/732-6400EDU_0921121012543.xml 14 7326400EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jc99dsmt 2017-11-14 07:07:14 true true internet/product_SKU jc99dsmt templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/732-6400EDU_0921121012543.xml LSE 2019-05-04 11:13:12 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972631979 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 11:13:12 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/732-6400EDU_0921121012543.xml templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/732-6400EDU_0921121012543.xml 7326400edu-aurum-plasmid-mini-purification-kit 7326400EDU Aurum Plasmid Mini Purification Kit Aurum Plasmid Mini Purification Kit Plasmid purification kit contains plasmid-binding mini columns, capless collection tubes, reagents, for 100 preps Plasmid purification kit contains plasmid-binding mini columns, capless collection tubes, reagents, for 100 preps Plasmid purification kit contains plasmid-binding mini columns, capless collection tubes, reagents, for 100 preps /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_thumb/global/732-6400EDU_tn.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_view/global/732-6400EDU_view.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_feature/global/732-6400EDU_feature.jpg Aurum™ Plasmid Mini Purification Kit #732-6400EDU RepEx Aurum™ Plasmid Mini Purification Kit #732-6400EDU Plasmid purification kit contains plasmid-binding mini columns, capless collection tubes, reagents, for 100 preps 7326400, 7326400EDU, aurum, plasmid, mini, dna, purification, sample, preparation, columns, capless, collection, tubes, reagents, science, education, products, classroom 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore N 0 ,VN <p>The seventh module in the Cloning and Sequencing Explorer Series, the Aurum™ Plasmid Mini Purification Module contains reagents to purify up to 20 µg of DNA from a plasmid miniculture. The kit includes spin columns, collection tubes, buffers, and curriculum. This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated/ 732-6400EDU_0921121012543_en.html /webroot/web/html/lse/products/SKUs/autopopulated//732-6400EDU_0921121012543_en.html 1141 <b>Kit contains sufficient reagents to purify DNA from 100 minicultures of plasmid-bearing bacteria</b>   1 0 1580 DNA-binding columns 100 1 0 2700 Capless collection tubes, 2.0 ml 100 1 0 2373 Resuspension solution, 25 ml 1 1 0 2374 Lysis solution, 25 ml 1 1 0 2375 Neutralization solution, 40 ml 1 1 0 2701 Wash solution, 5x, 25 ml 1 1 0 2377 Elution solution, 16 ml 1 1 0 2398 Curriculum, including teacher's guide, student manual, and graphic quick guide 1 1 0 false £233.00 false $ null true false true true false GBP true true true /prd/en/UK/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7326400EDU&quantity= false false /prd/en/UK/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7326400EDU&CartQuote=QT&quantity= 1711 false true false /prd/en/UK/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7326400EDU&AddToDefaultWishList=true&quantity= 41116100 233.00 7326400EDU

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(Discontinued) Sequencing and Bioinformatics Module

1665025EDU
Eighth module in Cloning and Sequencing Explorer Series classroom study kit, includes primers, plasmid, barcoded 96-well plate, test tubes, curriculum, and more, for 12 workstations; education use only (Sequencing service not included)

This product is no longer available. Please contact technical support for more information on replacement product.

Cloning and Sequencing Explorer Series Instruction Manual

1665001EDU
CD-ROM for use with Cloning and Sequencing Explorer series (1665000EDU), includes electronic version of instruction manual (PDF format); education use only

1665001EDU /templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/166-5001EDU_0921121012449.xml 1 1665001EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jc99dsos 2017-11-14 07:07:14 true true internet/product_SKU jc99dsos templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/166-5001EDU_0921121012449.xml LSE 2019-05-04 11:13:52 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972631643 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 11:13:52 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/166-5001EDU_0921121012449.xml templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/166-5001EDU_0921121012449.xml 1665001edu-cloning-sequencing-explorer-series-instruction-manual 1665001EDU Cloning and Sequencing Explorer Series Instruction Manual Cloning and Sequencing Explorer Series Instruction Manual CD-ROM for use with Cloning and Sequencing Explorer series (1665000EDU), includes electronic version of instruction manual (PDF format); education use only CD-ROM for use with Cloning and Sequencing Explorer series (#166-5000EDU), includes electronic version of instruction manual (PDF format); education use only CD-ROM for use with Cloning and Sequencing Explorer series (1665000EDU), includes electronic version of instruction manual (PDF format); education use only /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_thumb/global/166-5001EDU_tn.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_view/global/166-5001EDU_view.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_feature/global/166-5001EDU_feature.jpg Cloning and Sequencing Explorer Series Instruction Manual #166-5001EDU ,VN RepEx Cloning and Sequencing Explorer Series Instruction Manual #166-5001EDU CD-ROM for use with Cloning and Sequencing Explorer series (#166-5000EDU), includes electronic version of instruction manual (PDF format); education use only curriculum, guide, cd-rom 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore N 0 <p>The Cloning and Sequencing Explorer Series Instruction Manual is provided on a CD-ROM. Instruction manual, curriculum (including Teacher's Guide and Student Manual) and individual module instruction manuals are provided as PDFs. This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated/ 166-5001EDU_0921121012449_en.html /webroot/web/html/lse/products/SKUs/autopopulated//166-5001EDU_0921121012449_en.html Cloning and Sequencing Explorer Series curriculum resource CD 2682 1 1 0 false £33.74 false $ null false false true true false GBP true false true /prd/en/UK/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665001EDU&quantity= false false /prd/en/UK/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665001EDU&CartQuote=QT&quantity= 1711 false true false /prd/en/UK/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665001EDU&AddToDefaultWishList=true&quantity= 60101700 33.74 1665001EDU

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Number	Description	Options
10000069187	Bio-Rad Explorer Cloning and Sequencing Explorer Series, Ver G	Click to download
5773	Cloning and Sequencing Explorer Series Planning Guide, Bio-Rad Explorer™ , Ver C	Click to download
6672	Map of AP-Aligned Bio-Rad Kits with Essential Knowledge	Click to download
6673	Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories	Click to download
6602	UView™ 6x Loading Dye and Stain Guide, Bio-Rad Explorer™	Click to download

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