PureBlu™ Hoechst 33342 Nuclear Staining Dye #1351304

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1351304 [x-forwarded-proto] = [https] [accept-language] = [en-US,en;q=0.5] [x-forwarded-port] = [443] [x-forwarded-for] = [18.208.159.25, 10.232.2.111] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [seourl] = [/en-sg/sku/1351304-pureblu-hoechst-33342-nuclear-staining-dye] [x-amzn-trace-id] = [Root=1-5dab0a46-a7ae3f24b30c8bf1c1c2761d] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=1351304] [host] = [10.232.17.28:1776] [x-request-uri] = [/en-sg/sku/1351304-pureblu-hoechst-33342-nuclear-staining-dye] [connection] = [Keep-Alive] [accept-encoding] = [gzip] [user-agent] = [CCBot/2.0 (https://commoncrawl.org/faq/)] Catalog/ProductSKU pageStyleKey 1351304-pureblu-hoechst-33342-nuclear-staining-dye LSR en SG en-sg /prd 07/30/13 03:25 AM en_US true internet/generic 2019-05-04 20:39:39 /default/main/bio-rad/STAGING 1375179935592 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml page_EDUPriceOverlay /default/main/bio-rad/WORKAREA/default jddgmm0t 2017-11-14 06:29:46 true jddgmm0t templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml LSE N /default/main/bio-rad en 07/30/20 05:00 PM 1522700291361 2019-05-04 20:39:39 /default/main/bio-rad/WORKAREA/default/templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml edu-price-overlay 1 1375179935592 page_EDUPriceOverlay <div id="eduPriceOverlay" class="overlay" style="display: block;"> <p class="headerClassText">What is the education discount policy?</p> <p class="descClassText">For more than 15 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows high school and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices.</p> <p class="headerClassText">How do I apply for the education discount?</p> <p class="descClassText">If you are an educator at the high school or college level, visit our <a href="/en-us/education/education-discount-policy?ID=MRFKWO4VY">Education Discount Policy</a> page to establish an education account number.</p> </div> /webroot/web/html EDU_price_overlay.html /webroot/web/html/EDU_price_overlay.html sandeep Edu Edu Price Overlay Edu Price Overlay Content 07/30/13 03:25 AM 07/30/20 05:00 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE N 0 {"ecommerce":{"detail":{"products":{"price":"0","name":"PureBlu™ Hoechst 33342 Nuclear Staining Dye","variant":"BRC","id":"1351304","category":"BRC","brand":"BRC"}}},"event":"detail"} 1351304 06/19/14 02:49 PM en_US true internet/product_SKU 2019-05-04 15:34:03 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Cell-Imaging/Nuclear-Staining-Dyes/135-1304_1403215064.xml /default/main/bio-rad/WORKAREA/default jb42cv29 2017-11-14 07:07:54 true jb42cv29 templatedata/internet/product_SKU/data/LSR/Cell-Imaging/Nuclear-Staining-Dyes/135-1304_1403215064.xml LSR N /default/main/bio-rad en 06/19/24 02:53 PM 1556127197748 2019-05-04 15:34:03 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Cell-Imaging/Nuclear-Staining-Dyes/135-1304_1403215064.xml 1351304-pureblu-hoechst-33342-nuclear-staining-dye 1 1351304 PureBluHoechst33342NuclearStainDye PureBlu™ Hoechst 33342 Nuclear Staining Dye Pkg of 5 vials, 56 µg each, Hoechst 33342 nuclear staining dye powder /webroot/web/images/lsr/products/cell_imaging/sku_thumb/global/pureblu-hoechst-33342-nuclear-staining-dye-sku-135-1304-thumb.jpg /webroot/web/images/lsr/products/cell_imaging/sku_feature/global/pureblu-hoechst-33342-nuclear-staining-dye-sku-135-1304-detail.jpg PureBlu Hoechst 33342 Nuclear Staining Dye preview ,VN tommy a PureBlu™ Hoechst 33342 Nuclear Staining Dye 1351304, pureblu, hoechst, 33342, nuclear, staining, dye, powder, cell, nucleus, stain, fluorescence, microscopy, imaging 06/19/14 02:49 PM 06/19/24 02:53 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Cell-Imaging/Nuclear-Staining-Dyes N 0 <p>Use PureBlu Hoechst 33342 Dye for routine nuclear staining in fluorescence microscopy and cell imaging applications. PureBlu Hoechst 33342 Dye is a highly pure formulation of Hoechst 33342 in a user-friendly format allowing easy preparation of the working solution, with no weighing step and only a single dilution after resuspension.</p> <p>Hoechst 33342 is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. Hoechst 33342 permeates cell membranes and binds to the minor groove of A/T-rich dsDNA sequences, thus preferentially staining nuclei (Figure 1).</p> <p><img src="/webroot/web/images/lsr/products/cell_imaging/product_detail/global/molecular-binding-hoechst.jpg" alt="Hoechst 33342 binding dsDNA" /></p> <p class="caption"><strong>Fig. 1. Hoechst 33342 binding dsDNA.</strong><br />&nbsp;</p> <p>When bound to dsDNA, Hoechst 33342 has a maximum excitation wavelength in the ultraviolet range (350 nm), and the dye can be optimally detected in the blue channel with an emission maximum of 461 nm (Figure 2). The characteristic Stokes shift between excitation and emission wavelengths is fairly wide for Hoechst 33342, making this dye an optimal choice when good spectral separation is desired to reduce fluorescence interference, for example, in chromatin counterstaining for immunofluorescence microscopy.</p> <p><br /><img src="/webroot/web/images/lsr/products/cell_imaging/product_detail/global/hoechst-33342-emission-spectrum.gif" alt="Hoechst 33342 emission spectrum" /></p> <p class="caption"><strong>Fig. 2. Hoechst 33342 excitation and emission spectra.</strong><br />&nbsp;</p> <p>PureBlu Hoechst 33342 Nuclear Staining Dye is compatible with fixed and unfixed cells. Compared to DAPI, it exhibits greater permeability toward intact cell membranes and is preferred when conducting experiments with unfixed samples.</p> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/zoe-fluorescent-cell-imager">ZOE&trade; Fluorescent Cell Imaging System</a></li> <li><a href="/en-us/product/tc20-automated-cell-counter?ID=M7FBG34VY">TC20&trade; Automated Cell Counter</a></li> </ul> <h2 class="descHeader">More Information</h2> <p><a class="no-target-style" href="/webroot/web/pdf/lsr/literature/10043283.pdf" target="_blank" rel="noopener noreferrer">PureBlu&trade; DAPI Nuclear Staining Dye Product Insert</a></p> /webroot/web/html/lsr/products/SKUs 135-1304.html /webroot/web/html/lsr/products/SKUs/135-1304.html Molecular formula 2963 C<sub>27</sub>H<sub>28</sub>N<sub>6</sub>O·3HCl 1 0 Molecular weight 2964 561.93 1 0 Excitation/emission wavelengths 2897 350/461 nm 1 0 CAS Number 2961 23491-52-3 1 0 Purity 2036 >95% (HPLC purified) 1 0 Solubility 2962 Soluble in water and dimethyl sulfoxide (DMSO) 1 0 Storage and use 2877 –20ºC 1 0 Temperature stability 2251 Stable for 2 years at –20°C <br />After resuspension: stable for 1 year at –20°C or 6 months at 2–8°C 1 0 1351303 /templatedata/internet/product_SKU/data/LSR/Cell-Imaging/Nuclear-Staining-Dyes/135-1303_1403214125.xml 1450031 /templatedata/internet/product_SKU/data/LSR/Cell-Imaging/ZOE-Fluorescent-Cell-Imager/145-0031_1403030204.xml 1450102 /templatedata/internet/product_SKU/data/LSR/Cell-Counting/TC20-Automated-Cell-Counter/145-0102_1345072589.xml true null false $ null false false true true false BND true false true /prd/en/SG/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1351304&quantity= false false /prd/en/SG/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1351304&CartQuote=QT&quantity= 1108 false true false /prd/en/SG/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1351304&AddToDefaultWishList=true&quantity= 12171500 null 135-1304-MSDS PureBlu 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Pkg of 5 vials, 56 µg each, Hoechst 33342 nuclear staining dye powder

1351304 [x-forwarded-proto] = [https] [accept-language] = [en-US,en;q=0.5] [x-forwarded-port] = [443] [x-forwarded-for] = [18.208.159.25, 10.232.2.111] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [seourl] = [/en-sg/sku/1351304-pureblu-hoechst-33342-nuclear-staining-dye] [x-amzn-trace-id] = [Root=1-5dab0a46-a7ae3f24b30c8bf1c1c2761d] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=1351304] [host] = [10.232.17.28:1776] [x-request-uri] = [/en-sg/sku/1351304-pureblu-hoechst-33342-nuclear-staining-dye] [connection] = [Keep-Alive] [accept-encoding] = [gzip] [user-agent] = [CCBot/2.0 (https://commoncrawl.org/faq/)] Catalog/ProductSKU pageStyleKey 1351304-pureblu-hoechst-33342-nuclear-staining-dye LSR en SG en-sg /prd 07/30/13 03:25 AM en_US true internet/generic 2019-05-04 20:39:39 /default/main/bio-rad/STAGING 1375179935592 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml page_EDUPriceOverlay /default/main/bio-rad/WORKAREA/default jddgmm0t 2017-11-14 06:29:46 true jddgmm0t templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml LSE N /default/main/bio-rad en 07/30/20 05:00 PM 1522700291361 2019-05-04 20:39:39 /default/main/bio-rad/WORKAREA/default/templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml edu-price-overlay 1 1375179935592 page_EDUPriceOverlay <div id="eduPriceOverlay" class="overlay" style="display: block;"> <p class="headerClassText">What is the education discount policy?</p> <p class="descClassText">For more than 15 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows high school and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices.</p> <p class="headerClassText">How do I apply for the education discount?</p> <p class="descClassText">If you are an educator at the high school or college level, visit our <a href="/en-us/education/education-discount-policy?ID=MRFKWO4VY">Education Discount Policy</a> page to establish an education account number.</p> </div> /webroot/web/html EDU_price_overlay.html /webroot/web/html/EDU_price_overlay.html sandeep Edu Edu Price Overlay Edu Price Overlay Content 07/30/13 03:25 AM 07/30/20 05:00 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE N 0 {"ecommerce":{"detail":{"products":{"price":"0","name":"PureBlu™ Hoechst 33342 Nuclear Staining Dye","variant":"BRC","id":"1351304","category":"BRC","brand":"BRC"}}},"event":"detail"} 1351304 06/19/14 02:49 PM en_US true internet/product_SKU 2019-05-04 15:34:03 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Cell-Imaging/Nuclear-Staining-Dyes/135-1304_1403215064.xml /default/main/bio-rad/WORKAREA/default jb42cv29 2017-11-14 07:07:54 true jb42cv29 templatedata/internet/product_SKU/data/LSR/Cell-Imaging/Nuclear-Staining-Dyes/135-1304_1403215064.xml LSR N /default/main/bio-rad en 06/19/24 02:53 PM 1556127197748 2019-05-04 15:34:03 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Cell-Imaging/Nuclear-Staining-Dyes/135-1304_1403215064.xml 1351304-pureblu-hoechst-33342-nuclear-staining-dye 1 1351304 PureBluHoechst33342NuclearStainDye PureBlu™ Hoechst 33342 Nuclear Staining Dye Pkg of 5 vials, 56 µg each, Hoechst 33342 nuclear staining dye powder /webroot/web/images/lsr/products/cell_imaging/sku_thumb/global/pureblu-hoechst-33342-nuclear-staining-dye-sku-135-1304-thumb.jpg /webroot/web/images/lsr/products/cell_imaging/sku_feature/global/pureblu-hoechst-33342-nuclear-staining-dye-sku-135-1304-detail.jpg PureBlu Hoechst 33342 Nuclear Staining Dye preview ,VN tommy a PureBlu™ Hoechst 33342 Nuclear Staining Dye 1351304, pureblu, hoechst, 33342, nuclear, staining, dye, powder, cell, nucleus, stain, fluorescence, microscopy, imaging 06/19/14 02:49 PM 06/19/24 02:53 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Cell-Imaging/Nuclear-Staining-Dyes N 0 <p>Use PureBlu Hoechst 33342 Dye for routine nuclear staining in fluorescence microscopy and cell imaging applications. PureBlu Hoechst 33342 Dye is a highly pure formulation of Hoechst 33342 in a user-friendly format allowing easy preparation of the working solution, with no weighing step and only a single dilution after resuspension.</p> <p>Hoechst 33342 is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. Hoechst 33342 permeates cell membranes and binds to the minor groove of A/T-rich dsDNA sequences, thus preferentially staining nuclei (Figure 1).</p> <p><img src="/webroot/web/images/lsr/products/cell_imaging/product_detail/global/molecular-binding-hoechst.jpg" alt="Hoechst 33342 binding dsDNA" /></p> <p class="caption"><strong>Fig. 1. Hoechst 33342 binding dsDNA.</strong><br />&nbsp;</p> <p>When bound to dsDNA, Hoechst 33342 has a maximum excitation wavelength in the ultraviolet range (350 nm), and the dye can be optimally detected in the blue channel with an emission maximum of 461 nm (Figure 2). The characteristic Stokes shift between excitation and emission wavelengths is fairly wide for Hoechst 33342, making this dye an optimal choice when good spectral separation is desired to reduce fluorescence interference, for example, in chromatin counterstaining for immunofluorescence microscopy.</p> <p><br /><img src="/webroot/web/images/lsr/products/cell_imaging/product_detail/global/hoechst-33342-emission-spectrum.gif" alt="Hoechst 33342 emission spectrum" /></p> <p class="caption"><strong>Fig. 2. Hoechst 33342 excitation and emission spectra.</strong><br />&nbsp;</p> <p>PureBlu Hoechst 33342 Nuclear Staining Dye is compatible with fixed and unfixed cells. Compared to DAPI, it exhibits greater permeability toward intact cell membranes and is preferred when conducting experiments with unfixed samples.</p> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/zoe-fluorescent-cell-imager">ZOE&trade; Fluorescent Cell Imaging System</a></li> <li><a href="/en-us/product/tc20-automated-cell-counter?ID=M7FBG34VY">TC20&trade; Automated Cell Counter</a></li> </ul> <h2 class="descHeader">More Information</h2> <p><a class="no-target-style" href="/webroot/web/pdf/lsr/literature/10043283.pdf" target="_blank" rel="noopener noreferrer">PureBlu&trade; DAPI Nuclear Staining Dye Product Insert</a></p> /webroot/web/html/lsr/products/SKUs 135-1304.html /webroot/web/html/lsr/products/SKUs/135-1304.html Molecular formula 2963 C<sub>27</sub>H<sub>28</sub>N<sub>6</sub>O·3HCl 1 0 Molecular weight 2964 561.93 1 0 Excitation/emission wavelengths 2897 350/461 nm 1 0 CAS Number 2961 23491-52-3 1 0 Purity 2036 >95% (HPLC purified) 1 0 Solubility 2962 Soluble in water and dimethyl sulfoxide (DMSO) 1 0 Storage and use 2877 –20ºC 1 0 Temperature stability 2251 Stable for 2 years at –20°C <br />After resuspension: stable for 1 year at –20°C or 6 months at 2–8°C 1 0 1351303 /templatedata/internet/product_SKU/data/LSR/Cell-Imaging/Nuclear-Staining-Dyes/135-1303_1403214125.xml 1450031 /templatedata/internet/product_SKU/data/LSR/Cell-Imaging/ZOE-Fluorescent-Cell-Imager/145-0031_1403030204.xml 1450102 /templatedata/internet/product_SKU/data/LSR/Cell-Counting/TC20-Automated-Cell-Counter/145-0102_1345072589.xml true null false $ null false false true true false BND true false true /prd/en/SG/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1351304&quantity= false false /prd/en/SG/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1351304&CartQuote=QT&quantity= 1108 false true false /prd/en/SG/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1351304&AddToDefaultWishList=true&quantity= 12171500 null 135-1304-MSDS PureBlu 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Use PureBlu Hoechst 33342 Dye for routine nuclear staining in fluorescence microscopy and cell imaging applications. PureBlu Hoechst 33342 Dye is a highly pure formulation of Hoechst 33342 in a user-friendly format allowing easy preparation of the working solution, with no weighing step and only a single dilution after resuspension.

Hoechst 33342 is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. Hoechst 33342 permeates cell membranes and binds to the minor groove of A/T-rich dsDNA sequences, thus preferentially staining nuclei (Figure 1).

Hoechst 33342 binding dsDNA

Fig. 1. Hoechst 33342 binding dsDNA.
 

When bound to dsDNA, Hoechst 33342 has a maximum excitation wavelength in the ultraviolet range (350 nm), and the dye can be optimally detected in the blue channel with an emission maximum of 461 nm (Figure 2). The characteristic Stokes shift between excitation and emission wavelengths is fairly wide for Hoechst 33342, making this dye an optimal choice when good spectral separation is desired to reduce fluorescence interference, for example, in chromatin counterstaining for immunofluorescence microscopy.


Hoechst 33342 emission spectrum

Fig. 2. Hoechst 33342 excitation and emission spectra.
 

PureBlu Hoechst 33342 Nuclear Staining Dye is compatible with fixed and unfixed cells. Compared to DAPI, it exhibits greater permeability toward intact cell membranes and is preferred when conducting experiments with unfixed samples.

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PureBlu™ DAPI Nuclear Staining Dye Product Insert

Molecular formula C27H28N6O·3HCl
Molecular weight 561.93
Excitation/emission wavelengths 350/461 nm
CAS Number 23491-52-3
Purity >95% (HPLC purified)
Solubility Soluble in water and dimethyl sulfoxide (DMSO)
Storage and use –20ºC
Temperature stability Stable for 2 years at –20°C
After resuspension: stable for 1 year at –20°C or 6 months at 2–8°C
Number Description Options
Available Translation
PureBlu Hoechst 33342 Nuclear Staining Dye  (SDS)gene

Bulletin# 135-1304-MSDS

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