Overview
The pGLO Bacterial Transformation Kit for General Biology is a three-dimensional approach to the classic transformation lab. Teach core genetics concepts including gene expression and gene regulation using this hands-on and student-driven experimental design experience.
Features and Benefits
- Includes consumables and reagents for 12 laboratory workstations or 48 students
- Enables students to design and analyze two gene expression experiments
- Allows students to create and revise models to explain their observations
- Activities designed for three to five 50-minute class periods
Kit Activity Overview
- Observe fluorescent organisms and create models to explain observations
- Learn about plasmids and bacterial transformation
- Conduct a bacterial transformation experiment to collect data about the role of antibiotic selection markers
- Design and conduct and experiment to switch on the GFP gene in transformed bacteria
- Design a biosensor based on bacterial transformation to solve a real-world problem
Additional Teaching Resources for the pGLO Lab
pGLO Bacterial Transformation Kit for General Biology | pGLO Bacterial Transformation Kit | pGLO Transformation and Inquiry Kit for AP Biology | |
---|---|---|---|
A highly scaffolded three-dimensional approach to bacterial transformation and gene expression; students design their own step-wise experiments and write and revise models and scientific explanations. | A laboratory skills-based approach to bacterial transformation; students follow a robust protocol for a well-controlled experiment while analyzing the details of the technique. | An inquiry-based approach to bacterial transformation through experimental optimization; students ask their own questions and conduct multiple experiments to refine the parameters of the technique. | |
Primary curriculum concepts | Gene expression, gene regulation, antibiotic resistance, artificial selection, experimental design, laboratory skills | ||
Workstations | 12 | 8 | 8 |
Laboratory activities | 2 | 1 | 5 |
Class periods(50 min) | 3–5 | 2 | Flexible to fit a range of class periods |
Specifications
(2-4 students per workstation)
Ordering
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Accessories
120 V, compact incubator for growing bacteria, includes adjustable shelf, non-mercury thermometer, over-temperature protection, holds eighty 6.5 cm culture plates, CE approved; education use only
120 V, vortex mixer, 0–3,000 rpm, continuous or touch activation, includes general-purpose cup attachment, CE approved; education use only
120 V, 6 L water bath, ambient to 100°C, includes stainless steel tank and gable cover, over-temperature protection, non-mercury thermometer, CE approved; education use only
Stainless steel chamber and aluminum block offer rapid and even heating of up to 24 x 1.5 ml microcentrifuge tubes, CE approved; education use only
Pkg of 1, pocket-sized disposable UV light to illuminate Green Fluorescent Protein (GFP); education use only
Refills
Consumable reagents for pGLO Bacterial Transformation Kits, includes pGLO Plasmid, E. coli, nutrient broth, transformation solution, ampicillin, arabinose; LB agar powder (1660600EDU) not included; for education use only
60 mm agar plates; education use only