Every year Bio-Rad hosts professional seminars (including online Web seminars), user groups, and workshops. Our product and application experts are ready to help you learn ways to facilitate your research.
In need of some additional product training? We offer great supplementary workshops tailored to the needs of your research and the products that you use. Get the most out of your lab today!
Workshop topics include:
Speed3 Protein Workshop
Innovations for Fast Protein Electrophoresis, Blotting, and Imaging
Seeing is believing!
Would you like your protein electrophoresis, blotting and imaging to be fast, reproducible, and easy to use?
How about 30 minutes?
Can't believe that?
Then challenge us and ask for a workshop at your department!
In this workshop we can cover the following innovations for fast protein electrophoresis, blotting, and imaging, both in theory and in practice:
Total workshop duration 30-90 minutes — you pick the topics you want to learn about!
- TGX™ precast gels:
Long shelf-life and Laemmli buffer system - Shorter run times
- Easy handling
- Trans-Blot® Turbo™ transfer system:
Down to 3 min transfer time - High efficiency
- Up to 4 mini gels or 2 midi gels at once
- Stain-Free™ technology:
Visualise proteins in less than 5 minutes - No fixing, staining, or destaining
- Compatible with downstream analysis
- Gel Doc™ EZ imaging system:
Fully automated image acquisition and analysis - Modular design
- Superior image quality
- Hands-on experience:
Gels will be run, blotted, and analysed during the presentation
Total workshop duration 30-90 minutes — you pick the topics you want to learn about!
The workshop is free of charge and just needs a seminar room to be organised by the applicant.
qPCR Seminar
Best Practice Quantitative Real-Time PCR
A typical agenda:
Welcome
- Best practice qPCR: workflow and MIQE update
- BioStatistics: CFX Manager™ software and qbasePLUS
- Coffee break
- Fast qPCR:
- CFX96™ real-time PCR System
- SsoFast™ probes supermix
- Epigenetics — an introduction
- DNA methylation analysis
- EpiQ™ chromatin analysis kit: new epigenetic research tool
- Discussion
Your participation is free of charge.
qPCR and Gene Expression Analysis Courses
qPCR Assay Design and Sample Preparation
An advanced course with hands-on experience in the laboratory
Who will benefit from attending?
Real-Time PCR users who need advanced hints and tips for DNA and RNA sample preparation and experimental design of real-time PCR applications.
Objective
The objective of this course is to offer the opportunity for hands-on experience in the lab under the supervision of instructors. Best strategies and practices for effective sample preparation, efficient reverse transcription, generation of high-quality PCR data, and optimal qPCR assay design will be recommended.
Content
Nucleic acids isolation and purification
- Introduction to sample preparation techniques and principles
- Hands-on experience with different sample preparation methods
- Extraction from difficult matrices and small samples
- RNA and DNA quality control
- Automated electrophoresis in silico assay design: design your own assay
- Introduction to PCR and qPCR
- Primer and probe design
- Reverse transcription: guidelines and practical hands-on experience with cDNA synthesis
- Real-time quantitative PCR (qPCR) experimental guidelines and hands-on experience in the lab
Trainers: TA TAA Biocenter and Bio-Rad technical specialists
TATAA Biocenter is a commercial research provider that offers training and commissioned research within molecular diagnostics and gene expression analysis using real-time PCR and other molecular techniques to quantify nucleic acids. Their competence is based on knowledge and experience accumulated through years of research at leading European universities.
qPCR and Statistical Analysis of Gene Expression
An interactive course with statistical exercises based on real data, including data sets provided by participants.
Who will benefit from attending?
Real-Time PCR users who need advanced knowledge in biostatistics in the field of gene expression analysis.
Objective
The objective of this course is to provide an understanding of data processing and statistical methods used for analysis of data obtained by real-time PCR. A key ingredient of the course is practical exercises based on real data, including data sets provided by participants. The common experimental designs are introduced and data from such studies is analysed by participants under the supervision of instructors. Throughout the course participants will become familiar with the most common experimental cases and create Excel-based templates for data analysis, which they can take home for their own use. In addition, an overview of suitable qPCR software tools will be given.
Content
- Introduction to statistics
- Amplification process
- Threshold level and the Cq value
- Computation of the copy number
- Computation of the expression value
- Normalisation methods
- Selection and normalisation with reference genes
- Statistical tests in analysis of real-time PCR data (demonstration versions of software will be provided)
- qbasePLUS, etc.
- MIQE guidelines
Trainers: Jo Vandesompele, Jan Hellemans, and Bio-Rad technical specialists
Jo has been a professor in bioinformatics and biocomputing at the Center for Medical Genetics at Ghent University since 2007. He is author of various pioneering publications in the area of real-time PCR. Together with Jan Hellemans he developed advanced and universally applicable quantification methods for automated and accurate qPCR data analysis. He is also the co-founder of Biogazelle, the real-time PCR data-analysis company.
Jan Hellemans has a masterís degree in biotechnology (2000, Univ of Gent) and a PhD in medical genetics (2007, Univ of Gent). Dr Hellemans is author of several scientific articles in highly ranked journals and is active as a teacher of qPCR courses. He supervises a core facility for automated DNA isolation, sequencing, genotyping, and high-throughput real-time PCR. Jan is the designer and programmer of the qBase software that is used for qPCR data analysis.
Excel is a trademark of Microsoft Corporation.
For more information on these seminars and workshops please contact your local Bio-Rad office, which will be happy to provide you with further details.
Call us at 00 800 00 BIORAD (00 800 00 24 67 23) or +351 21 47 27 717
Technical Support: 00 800 00 BIORAD (00 800 00 24 67 23) or +351 21 47 27 717
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