Reliance One-Step Multiplex RT-qPCR Supermix, 200 x 20 µl rxns, 1 ml #12010176

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12010176 [x-forwarded-proto] = [https] [accept-language] = [en-US,en;q=0.5] [x-forwarded-port] = [443] [x-forwarded-for] = [35.168.112.145, 10.232.3.31] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [seourl] = [/en-fi/sku/12010176-reliance-one-step-multiplex-rt-qpcr-supermix-200-x-20-ul-rxns-1-ml] [x-amzn-trace-id] = [Root=1-5f0a2e87-3fb05d56b9608ac4098e8da6] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=12010176] [host] = [10.232.1.21:1776] [x-request-uri] = [/en-fi/sku/12010176-reliance-one-step-multiplex-rt-qpcr-supermix-200-x-20-ul-rxns-1-ml] [connection] = [Keep-Alive] [accept-encoding] = [br,gzip] [user-agent] = [CCBot/2.0 (https://commoncrawl.org/faq/)] Catalog/ProductSKU pageStyleKey 12010176-reliance-one-step-multiplex-rt-qpcr-supermix-200-x-20-ul-rxns-1-ml LSR en FI en-fi /prd false 07/30/13 03:25 AM en_US true internet/generic 2020-01-17 15:39:20 /default/main/bio-rad/STAGING 1375179935592 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml page_EDUPriceOverlay /default/main/bio-rad/WORKAREA/default jddgmm0t 2017-11-14 06:29:46 true jddgmm0t templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml LSE N /default/main/bio-rad en 07/30/20 05:00 PM 1557002494330 2020-01-17 15:39:20 /default/main/bio-rad/WORKAREA/default/templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml edu-price-overlay 1 1375179935592 page_EDUPriceOverlay <div id="eduPriceOverlay" class="overlay" style="display: block;"> <p class="headerClassText">What is the education discount policy?</p> <p class="descClassText">For more than 20 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows K&ndash;12 and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices. These discounted products are indicated by an EDU suffix (for example, 1660003EDU).</p> <p class="headerClassText">How do I apply for the education discount?</p> <p class="descClassText">If you are an educator at the K&ndash;12 or college level, contact your local <a href="/en-au/contact-us">Customer Service</a> office to establish an education account number.</p> </div> 0 /webroot/web/html EDU_price_overlay.html /webroot/web/html/EDU_price_overlay.html sandeep Edu Edu Price Overlay Edu Price Overlay Content 07/30/13 03:25 AM 07/30/20 05:00 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE N 0 {"ecommerce":{"detail":{"products":{"price":"\u20ac470.00","name":"Reliance One-Step Multiplex RT-qPCR Supermix, 200 x 20 µl rxns, 1 ml","variant":"BRC","id":"12010176","category":"BRC","brand":"BRC"}}},"event":"detail"} 12010176 04/08/19 08:21 PM en_US true internet/product_SKU 2020-05-25 00:38:40 /default/main/bio-rad/STAGING AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12010176_1554761350.xml /default/main/bio-rad/WORKAREA/default jthvlism 2019-04-08 22:09:10 true jthvlism templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12010176_1554761350.xml LSR N /default/main/bio-rad en 04/08/36 10:26 AM 1590110739175 2020-05-25 00:38:40 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12010176_1554761350.xml 12010176-reliance-one-step-multiplex-rt-qpcr-supermix-200-x-20-ul-rxns-1-ml 1 12010176 Reliance One-Step Multiplex Supermix Reliance One-Step Multiplex RT-qPCR Supermix, 200 x 20 µl rxns, 1 ml 1 ml (1 x 1 ml vial), 4x one-step RT-qPCR supermix, contains Reliance Reverse Transcriptase, blend of high-performance DNA polymerases including Sso7d fusion polymerase, universal reference dye, stabilizer, and reaction buffer, for 200 x 20 μl rxns 1 ml (1 x 1 ml vial), 4x one-step RT-qPCR supermix, contains Reliance Reverse Transcriptase, blend of high-performance DNA polymerases including Sso7d fusion polymerase, universal reference dye, stabilizer, and reaction buffer, for 200 x 20 μl rxns /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/12010176_th.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/12010176.jpg preview Anton Ross Reliance One-Step Multiplex Supermix 1 ml | Bio-Rad.com Use Bio-Rad's 1 ml Reliance One-Step Multiplex Supermix for sensitive amplification of up to 5 targets in a single reaction. 12010176, reliance, supermix, one-step multiplex supermix, pcr, amplification 04/08/19 08:21 PM 04/08/36 10:26 AM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <div class="corner-box corner-box-promo"><a href="http://loyaltyrewards.bio-rad.com/" target="_blank" rel="noopener noreferrer" class=""><img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/cfx-loyalty-program-icon.jpg" border="0" alt="CFX Loyalty Program" /></a> <p>Earn a free one-year service contract on your CFX System with purchase of Reagents.</p> <a href="http://loyaltyrewards.bio-rad.com/" target="_blank" rel="noopener noreferrer" class="new-target-image" title="Link opens in new window"><strong>CFX Loyalty Program</strong></a></div> <p class="wraptxt">Use the Reliance One-Step Multiplex Supermix for sensitive amplification of up to 5 targets in a single reaction. Expertly formulated with high-performance enzymes and advanced reaction buffer, this robust supermix delivers reproducible RT-qPCR performance in a multiplex fashion, including with challenging templates, difficult targets or in the presence of PCR inhibitors. With its exceptional room temperature stability for assembled reactions, it can be easily integrated into an automated workflow. Reliance One-Step Multiplex Supermix is an ideal solution for multiplexed RT-qPCR, especially for pathogen/viral detection and high throughput screening applications.</p> <img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/reliance-1-step-th.png" /> <div class="pdp-header"> <h2 class="overview_header">Features and Benefits</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/exceptional-performance-5-target-multiplex.html')">Sensitive, reproducible multiplexing of up to 5 targets</a><span>&nbsp;</span>&mdash; get the most out of your samples while reducing costs</li> <li><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/unparalleled-stability-assembled-rxns.html')">24-hour assembled reaction stability</a><span>&nbsp;</span>at room temperature &mdash; enables the use in an automated workflow to maximize throughput</li> <li>Convenient one-tube supermix &mdash; for minimizing reaction setup time and handling errors</li> <li>4x concentrated formulation &mdash; increase detection sensitivity by maximizing input volume</li> <li><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/Enhanced_Tolerance_Inhibitors_ov.html')">Tolerant to common PCR inhibitors</a><span>&nbsp;</span>&mdash; for use with lysates or samples of varying quality</li> </ul> <div class="pdp-header"> <h2 class="overview_header">Exceptional Performance of 5-target Multiplex</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <div class="pdp-desc-graph"><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/exceptional-performance-5-target-multiplex.html')"><img src="/webroot/web/images/lsr/products/amplification_pcr/product_overlay_content/global/exceptional-5plex-performance-th.jpg" alt="Exceptional 5-plex Performance" width="307" height="auto" /></a><br /> <div class="pdp-desc-graph-caption"><small><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/exceptional-performance-5-target-multiplex.html')">View 5-target multiplex data</a></small></div> </div> <div class="pdp-desc-summary"> <p>Reliance One-Step Multiplex Supermix enables researchers to get the most data out of their samples. Optimally formulated with a novel proprietary Reliance Reverse Transcriptase and robust DNA polymerases blend, this supermix amplifies up to 5 targets across a broad linear dynamic range from 1 pg to 1 &micro;g of RNA input with maximum sensitivity and efficiency.</p> </div> <div class="pdp-header"> <h2 class="overview_header">Unparalleled Room Temperature Stability for Up To 24 Hours</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <div class="pdp-desc-graph"><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/unparalleled-stability-assembled-rxns.html')"><img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/exceptional-room-temp-stability-icon.jpg" alt="Exceptional Room Temperature Stability" width="207" height="auto" style="padding-left: 100px;" /></a><br /> <div class="pdp-desc-graph-caption"><small><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/unparalleled-stability-assembled-rxns.html')">View assembled reaction stability data</a></small></div> </div> <div class="pdp-desc-summary"> <p>Reliance One-Step Multiplex Supermix is developed with high-throughput researchers in mind. Whether you want to queue up plates ahead of time or use an automated workflow, Reliance One-Step Multiplex Supermix will maintain its performance even after 24 hour incubation of assembled reactions.</p> </div> <br /> <br /> <div class="pdp-header"> <h2 class="overview_header">Efficient Co-detection of RNA and DNA Targets</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <div class="pdp-desc-graph"><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/co-detection-rna-dna-target-in-5plex-rxn.html')"><img src="/webroot/web/images/lsr/products/amplification_pcr/product_overlay_content/global/codetection-of-dna-rna-th.jpg" alt="Co-detection of DNA and RNA" width="307" height="auto" /></a><br /> <div class="pdp-desc-graph-caption"><small><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/co-detection-rna-dna-target-in-5plex-rxn.html')">View co-detection of RNA and DNA data</a></small></div> </div> <div class="pdp-desc-summary"> <p>Reliance One-Step Multiplex Supermix is designed to efficiently detect both RNA and DNA targets in the same reaction. Whether samples are tested for both RNA and DNA viruses at the same time or are run with internal controls, Reliance One-Step Multiplex Supermix reliably amplifies multiple targets regardless of their nature.</p> <small><a class="documentation-pdf-icon no-target-style" href="/webroot/web/pdf/lsr/literature/Bulletin_7196.pdf">View Application Note</a> (PDF 143KB)</small></div> <div class="pdp-header"> <h2 class="overview_header">Streamline Your Workflow</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li><a href="../product/cell-lysis-rt-qpcr-kits?ID=NVENZGE8Z">SingleShot Cell Lysis Kit</a>, for the generation of lysates for use in qPCR or western blots</li> <li><a href="../product/primepcr-pcr-primers-assays-arrays?ID=M0HROA15">PrimePCR Probe Assays</a> for multiplexing gene expression targets with minimal cross-reactivity and maximum transcript coverage</li> <li><a href="../product/cfx-automation-system-ii?ID=KR44O415">CFX Automation System II</a> for walk-away high-throughput qPCR operation</li> </ul> <div class="pdp-header"> <h2 class="overview_header">4x Reliance One-Step Multiplex Supermix is Formulated with:</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li>Optimal blend of Reliance chimeric reverse transcriptase* and high-performance DNA polymerases including Sso7d fusion DNA polymerase**</li> <li>4X reaction buffer containing dNTPs, RNase inhibitor, and other reaction optimizing components</li> <li>Reaction stabilizers</li> <li>Patented*** universal reference dye</li> </ul> <div class="pdp-header"> <h2 class="overview_header">Applications and Uses</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li>Gene expression analysis</li> <li>Viral/pathogen detection&nbsp;(RNA viruses, DNA viruses, bacterial viruses, and fungal viruses)</li> <li>Diagnostic research</li> <li>High-throughput screening&nbsp;(small molecules, drug discovery, dosage experiment, CRISPR screening, and toxicology study)</li> </ul> <div class="pdp-header"> <h2 class="overview_header">Packaging Options</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="middle"><strong>Kit Size</strong></td> <td valign="middle"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="middle">1 ml (1 x 1 ml vial), 200 x 20 &mu;l reactions</td> <td valign="middle">12010176</td> </tr> <tr> <td valign="middle">5 ml (5 x 1 ml vial), 1,000 x 20 &mu;l reactions</td> <td valign="middle"><a href="/en-us/sku/12010220-reliance-one-step-multiplex-supermix-1000-x-20-ul-rxns-5-ml?ID=12010220">12010220</a></td> </tr> <tr class="pd_colorbackground"> <td valign="middle">10 ml (2 x 5 ml vial), 2,000 x 20 &mu;l reactions</td> <td valign="middle"><a href="/en-us/sku/12010221-reliance-one-step-multiplex-supermix-2000-x-20-ul-rxns-10-ml?ID=12010221">12010221</a></td> </tr> </tbody> </table> <div class="pdp-header"> <h2 class="overview_header" style="margin: 10px 0 0 10px; clear: both;">Instrument Compatibility</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <p>Reliance One-Step Multiplex Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td colspan="2" valign="middle"><strong>Systems</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">Applied Biosystems</td> <td valign="top"> <ul> <li>StepOne/StepOnePlus</li> <li>7500, ViiA 7</li> <li>7000, 7300, 7700, 7900HT, QuantStudio Systems</li> </ul> </td> </tr> <tr> <td valign="top">Stratagene</td> <td valign="top"> <ul> <li>Mx3000P, 3005P, 4000</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Eppendorf</td> <td valign="top"> <ul> <li>Mastercycler ep realplex 2, 4</li> </ul> </td> </tr> <tr> <td valign="top">QIAGEN</td> <td valign="top"> <ul> <li>Corbett Rotor-Gene 3000, 6000, Q</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Roche</td> <td valign="top"> <ul> <li>LightCycler 480, 96</li> <li>LightCycler 1.0, 1.5, 2.0</li> </ul> </td> </tr> </tbody> </table> <div class="pdp-header"> <h2 class="overview_header">Choose the Right One-Step RT qPCR Product for Your Needs</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="top">&nbsp;</td> <td valign="top"><strong>Reliance One-Step Multiplex Supermix</strong></td> <td valign="top"><strong>iTaq Universal One-Step Probe Kit</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top"><strong>Key Features</strong></td> <td valign="top"> <ul> <li>Sensitive, efficient amplification of up to 5 targets</li> <li>Room temperature stable up to 24 hours</li> </ul> </td> <td valign="top"> <ul> <li>Reliable amplification of up to 2 targets</li> <li>Compatible with a fast protocol</li> </ul> </td> </tr> <tr> <td valign="top"><strong>Ideal for</strong></td> <td valign="top"> <ul> <li>Viral/pathogen detection in high-multiplex reaction</li> <li>High throughput screening</li> </ul> </td> <td valign="top"> <ul> <li>Viral/pathogen detection</li> <li>Singleplex and duplex reactions</li> </ul> </td> </tr> </tbody> </table> <div class="pdp-header"> <h2 class="overview_header">Related Products</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li><a href="../product/cell-lysis-rt-qpcr-kits?ID=NVENZGE8Z">Cell Lysis Kits</a></li> <li><a href="../category/rna-isolation?ID=2b86ef8e-07b5-4549-a544-0293620bb005">RNA Isolation Kits</a></li> <li><a href="../product/primepcr-pcr-primers-assays-arrays?ID=M0HROA15">PrimePCR Predesigned Probe qPCR Assays for Gene Expression</a> (Available in the U.S., Canada, E.U., China, Australia, New Zealand, Singapore, Taiwan, Hong Kong, Israel, South Africa, Thailand, Macau, Korea, and Japan.)</li> <li><a href="../product/cfx-automation-system-ii?ID=KR44O415">Real-Time PCR Automation System</a></li> </ul> <div class="pdp-header"> <h2 class="overview_header" style="margin: 10px 0 0 10px; clear: both;">Supporting Documents</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li>Amplification Reagents and Plastics <a href="/webroot/web/pdf/lsr/literature/Bulletin_6090.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">Brochure</a></li> <li>SingleShot Cell Lysis Kit <a href="/webroot/web/pdf/lsr/literature/10000112808.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">Instruction Manual</a></li> <li>One-Step Multiplex RT-qPCR Using PrimePCR Probe Assays <a href="/webroot/web/pdf/lsr/literature/Bulletin_7197.pdf" class="no-target-style">Protocol</a></li> </ul> <div class="pdp-header"> <h2 class="overview_header" style="margin: 10px 0 0 10px; clear: both;">Online Resources</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <p><a href="/en-us/applications-technologies/what-real-time-pcr-qpcr?ID=LUSO4W8UU" target="_blank" rel="noopener noreferrer" class="new-target-image" title="Link opens in new window">What is Real-Time PCR (qPCR)?</a></p> <div class="pdp-header"> <h2 class="overview_header" style="margin: 10px 0 0 10px; clear: both;">More Information</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <p>To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step <a href="/en-us/applications-technologies/qpcr-assay-design-optimization?ID=LUSO7RIVK">design and optimization protocols</a>. We also provide insights into analysis of <a href="/en-us/applications-technologies/real-time-pcr-data-analysis?ID=LUSOA9LPT">qPCR data</a> and <a href="/webroot/web/pdf/lsr/literature/10031339.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">troubleshooting reactions</a>.</p> <p>* Patent pending<br />** U.S. patents 6,627,424; 7,541,170; and 7,560,260.<br /> *** US patent 9,493,824</p> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global 12010176.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/12010176.html Product format 3184 Single-tube, ready-to use supermix 1 0 Concentration  1484 4x concentrated 1 0 Detection chemistry 3185 Probes 1 0 Multiplex capability 3183 Up to 5 targets 1 0 Assembled reaction stability 3181 Up to 24 hours 1 0 Inhibitor tolerance 3180 Tested with ethanol and hematin. Also compatible with up to 10 µl of crude lysates 1 0 5’ to 3’ exonuclease activity of the enzyme(s) 3182 Yes 1 0 Hot-start, activation time 3189 Yes, 10 min 1 0 Sample input 3179 1 pg to 1 µg 1 0 Sensitivity 2137 10 copies in a 5-plex reaction 1 0 Reference dye 3186 Tented universal dye preblended in the supermix 1 0 Instrument compatibility 2752 Both ROX-dependent (high- and low-) and -independent instruments 1 0 Guaranteed shelf life at –20° 3104 12 months 1 0 Shelf life at 4°C (after thawing) 3105 1 week 1 0 false €470.00 false null true false true true false EUR true true true /prd/en/FI/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12010176&quantity= false false /prd/en/FI/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12010176&CartQuote=QT&quantity= 1468 false true false /prd/en/FI/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12010176&AddToDefaultWishList=true&quantity= 41106300 470.00 SKU detail page 120-10176-MSDS Reliance One-Step Multiplex Supermix Australia English /webroot/web/pdf/WWMSDS/LSGC/AUS/AUS_ENG_12010176.pdf Korea Korean /webroot/web/pdf/WWMSDS/LSGC/ROK/ROK_KOR_12010176.pdf Belgium French /webroot/web/pdf/WWMSDS/LSGC/B/B_FR_12010176.pdf Italy Spanish /webroot/web/pdf/WWMSDS/LSGC/ISL/ISL_ES_12010176.pdf Mexico Spanish /webroot/web/pdf/WWMSDS/LSGC/MEX/MEX_ES_12010176.pdf Canada French /webroot/web/pdf/WWMSDS/LSGC/FCN/FCN_FR_12010176.pdf The Netherlands Dutch /webroot/web/pdf/WWMSDS/LSGC/NL/NL_NL_12010176.pdf Russian Federation Russian /webroot/web/pdf/WWMSDS/LSGC/RUS/RUS_RUS_12010176.pdf Hungary English /webroot/web/pdf/WWMSDS/LSGC/HE/HE_ENG_12010176.pdf Hungary Hungarian /webroot/web/pdf/WWMSDS/LSGC/H/H_UNG_12010176.pdf Brazil Portuguese /webroot/web/pdf/WWMSDS/LSGC/BRA/BRA_PT_12010176.pdf Germany German /webroot/web/pdf/WWMSDS/LSGC/D/D_D_12010176.pdf Czech Republic Czech /webroot/web/pdf/WWMSDS/LSGC/CZ/CZ_TS_12010176.pdf Denmark Danish /webroot/web/pdf/WWMSDS/LSGC/DK/DK_DA_12010176.pdf Denmark English /webroot/web/pdf/WWMSDS/LSGC/DKE/DKE_ENG_12010176.pdf Turkey Turkish /webroot/web/pdf/WWMSDS/LSGC/TR/TR_TR_12010176.pdf Germany English /webroot/web/pdf/WWMSDS/LSGC/DE/DE_ENG_12010176.pdf Poland Polish /webroot/web/pdf/WWMSDS/LSGC/PL/PL_POL_12010176.pdf Belgium English /webroot/web/pdf/WWMSDS/LSGC/BE/BE_ENG_12010176.pdf Hong Kong English /webroot/web/pdf/WWMSDS/LSGC/HK/HK_ENG_12010176.pdf Italy Italian /webroot/web/pdf/WWMSDS/LSGC/I/I_IT_12010176.pdf Portugal Portuguese /webroot/web/pdf/WWMSDS/LSGC/P/P_PT_12010176.pdf Ireland English /webroot/web/pdf/WWMSDS/LSGC/IE/IE_ENG_12010176.pdf Canada English /webroot/web/pdf/WWMSDS/LSGC/CDN/CDN_USA_12010176.pdf USA English /webroot/web/pdf/WWMSDS/LSGC/USA/USA_USA_12010176.pdf China Chinese /webroot/web/pdf/WWMSDS/LSGC/RC/RC_ZH_12010176.pdf Spain Spanish /webroot/web/pdf/WWMSDS/LSGC/E/E_ES_12010176.pdf Sweden Swedish /webroot/web/pdf/WWMSDS/LSGC/S/S_S_12010176.pdf Singapore English /webroot/web/pdf/WWMSDS/LSGC/SGP/SGP_ENG_12010176.pdf Czech Republic English /webroot/web/pdf/WWMSDS/LSGC/CZE/CZE_ENG_12010176.pdf United Kingdom English /webroot/web/pdf/WWMSDS/LSGC/GB/GB_ENG_12010176.pdf Austria English /webroot/web/pdf/WWMSDS/LSGC/AE/AE_ENG_12010176.pdf Sweden English /webroot/web/pdf/WWMSDS/LSGC/SE/SE_ENG_12010176.pdf France English /webroot/web/pdf/WWMSDS/LSGC/FE/FE_ENG_12010176.pdf New Zealand English /webroot/web/pdf/WWMSDS/LSGC/NZ/NZ_ENG_12010176.pdf Switzerland German /webroot/web/pdf/WWMSDS/LSGC/CH/CH_D_12010176.pdf China English /webroot/web/pdf/WWMSDS/LSGC/PRC/PRC_ENG_12010176.pdf India English /webroot/web/pdf/WWMSDS/LSGC/IN/IN_ENG_12010176.pdf Switzerland English /webroot/web/pdf/WWMSDS/LSGC/CHE/CHE_ENG_12010176.pdf Korea English /webroot/web/pdf/WWMSDS/LSGC/KOR/KOR_ENG_12010176.pdf Suomi Finnish /webroot/web/pdf/WWMSDS/LSGC/SF/SF_SF_12010176.pdf true Israel English /webroot/web/pdf/WWMSDS/LSGC/IL/IL_ENG_12010176.pdf Poland English /webroot/web/pdf/WWMSDS/LSGC/POL/POL_ENG_12010176.pdf France French /webroot/web/pdf/WWMSDS/LSGC/F/F_FR_12010176.pdf The Netherlands English /webroot/web/pdf/WWMSDS/LSGC/NLE/NLE_ENG_12010176.pdf Austria German /webroot/web/pdf/WWMSDS/LSGC/A/A_D_12010176.pdf Switzerland French /webroot/web/pdf/WWMSDS/LSGC/CHF/CHF_FR_12010176.pdf Norway Norwegian /webroot/web/pdf/WWMSDS/LSGC/N/N_N_12010176.pdf

1 ml (1 x 1 ml vial), 4x one-step RT-qPCR supermix, contains Reliance Reverse Transcriptase, blend of high-performance DNA polymerases including Sso7d fusion polymerase, universal reference dye, stabilizer, and reaction buffer, for 200 x 20 μl rxns

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These discounted products are indicated by an EDU suffix (for example, 1660003EDU).</p> <p class="headerClassText">How do I apply for the education discount?</p> <p class="descClassText">If you are an educator at the K&ndash;12 or college level, contact your local <a href="/en-au/contact-us">Customer Service</a> office to establish an education account number.</p> </div> 0 /webroot/web/html EDU_price_overlay.html /webroot/web/html/EDU_price_overlay.html sandeep Edu Edu Price Overlay Edu Price Overlay Content 07/30/13 03:25 AM 07/30/20 05:00 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE N 0 {"ecommerce":{"detail":{"products":{"price":"\u20ac470.00","name":"Reliance One-Step Multiplex RT-qPCR Supermix, 200 x 20 µl rxns, 1 ml","variant":"BRC","id":"12010176","category":"BRC","brand":"BRC"}}},"event":"detail"} 12010176 04/08/19 08:21 PM en_US true internet/product_SKU 2020-05-25 00:38:40 /default/main/bio-rad/STAGING AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12010176_1554761350.xml /default/main/bio-rad/WORKAREA/default jthvlism 2019-04-08 22:09:10 true jthvlism templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12010176_1554761350.xml LSR N /default/main/bio-rad en 04/08/36 10:26 AM 1590110739175 2020-05-25 00:38:40 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12010176_1554761350.xml 12010176-reliance-one-step-multiplex-rt-qpcr-supermix-200-x-20-ul-rxns-1-ml 1 12010176 Reliance One-Step Multiplex Supermix Reliance One-Step Multiplex RT-qPCR Supermix, 200 x 20 µl rxns, 1 ml 1 ml (1 x 1 ml vial), 4x one-step RT-qPCR supermix, contains Reliance Reverse Transcriptase, blend of high-performance DNA polymerases including Sso7d fusion polymerase, universal reference dye, stabilizer, and reaction buffer, for 200 x 20 μl rxns 1 ml (1 x 1 ml vial), 4x one-step RT-qPCR supermix, contains Reliance Reverse Transcriptase, blend of high-performance DNA polymerases including Sso7d fusion polymerase, universal reference dye, stabilizer, and reaction buffer, for 200 x 20 μl rxns /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/12010176_th.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/12010176.jpg preview Anton Ross Reliance One-Step Multiplex Supermix 1 ml | Bio-Rad.com Use Bio-Rad's 1 ml Reliance One-Step Multiplex Supermix for sensitive amplification of up to 5 targets in a single reaction. 12010176, reliance, supermix, one-step multiplex supermix, pcr, amplification 04/08/19 08:21 PM 04/08/36 10:26 AM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <div class="corner-box corner-box-promo"><a href="http://loyaltyrewards.bio-rad.com/" target="_blank" rel="noopener noreferrer" class=""><img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/cfx-loyalty-program-icon.jpg" border="0" alt="CFX Loyalty Program" /></a> <p>Earn a free one-year service contract on your CFX System with purchase of Reagents.</p> <a href="http://loyaltyrewards.bio-rad.com/" target="_blank" rel="noopener noreferrer" class="new-target-image" title="Link opens in new window"><strong>CFX Loyalty Program</strong></a></div> <p class="wraptxt">Use the Reliance One-Step Multiplex Supermix for sensitive amplification of up to 5 targets in a single reaction. Expertly formulated with high-performance enzymes and advanced reaction buffer, this robust supermix delivers reproducible RT-qPCR performance in a multiplex fashion, including with challenging templates, difficult targets or in the presence of PCR inhibitors. With its exceptional room temperature stability for assembled reactions, it can be easily integrated into an automated workflow. Reliance One-Step Multiplex Supermix is an ideal solution for multiplexed RT-qPCR, especially for pathogen/viral detection and high throughput screening applications.</p> <img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/reliance-1-step-th.png" /> <div class="pdp-header"> <h2 class="overview_header">Features and Benefits</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/exceptional-performance-5-target-multiplex.html')">Sensitive, reproducible multiplexing of up to 5 targets</a><span>&nbsp;</span>&mdash; get the most out of your samples while reducing costs</li> <li><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/unparalleled-stability-assembled-rxns.html')">24-hour assembled reaction stability</a><span>&nbsp;</span>at room temperature &mdash; enables the use in an automated workflow to maximize throughput</li> <li>Convenient one-tube supermix &mdash; for minimizing reaction setup time and handling errors</li> <li>4x concentrated formulation &mdash; increase detection sensitivity by maximizing input volume</li> <li><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/Enhanced_Tolerance_Inhibitors_ov.html')">Tolerant to common PCR inhibitors</a><span>&nbsp;</span>&mdash; for use with lysates or samples of varying quality</li> </ul> <div class="pdp-header"> <h2 class="overview_header">Exceptional Performance of 5-target Multiplex</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <div class="pdp-desc-graph"><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/exceptional-performance-5-target-multiplex.html')"><img src="/webroot/web/images/lsr/products/amplification_pcr/product_overlay_content/global/exceptional-5plex-performance-th.jpg" alt="Exceptional 5-plex Performance" width="307" height="auto" /></a><br /> <div class="pdp-desc-graph-caption"><small><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/exceptional-performance-5-target-multiplex.html')">View 5-target multiplex data</a></small></div> </div> <div class="pdp-desc-summary"> <p>Reliance One-Step Multiplex Supermix enables researchers to get the most data out of their samples. Optimally formulated with a novel proprietary Reliance Reverse Transcriptase and robust DNA polymerases blend, this supermix amplifies up to 5 targets across a broad linear dynamic range from 1 pg to 1 &micro;g of RNA input with maximum sensitivity and efficiency.</p> </div> <div class="pdp-header"> <h2 class="overview_header">Unparalleled Room Temperature Stability for Up To 24 Hours</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <div class="pdp-desc-graph"><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/unparalleled-stability-assembled-rxns.html')"><img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/exceptional-room-temp-stability-icon.jpg" alt="Exceptional Room Temperature Stability" width="207" height="auto" style="padding-left: 100px;" /></a><br /> <div class="pdp-desc-graph-caption"><small><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/unparalleled-stability-assembled-rxns.html')">View assembled reaction stability data</a></small></div> </div> <div class="pdp-desc-summary"> <p>Reliance One-Step Multiplex Supermix is developed with high-throughput researchers in mind. Whether you want to queue up plates ahead of time or use an automated workflow, Reliance One-Step Multiplex Supermix will maintain its performance even after 24 hour incubation of assembled reactions.</p> </div> <br /> <br /> <div class="pdp-header"> <h2 class="overview_header">Efficient Co-detection of RNA and DNA Targets</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <div class="pdp-desc-graph"><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/co-detection-rna-dna-target-in-5plex-rxn.html')"><img src="/webroot/web/images/lsr/products/amplification_pcr/product_overlay_content/global/codetection-of-dna-rna-th.jpg" alt="Co-detection of DNA and RNA" width="307" height="auto" /></a><br /> <div class="pdp-desc-graph-caption"><small><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/co-detection-rna-dna-target-in-5plex-rxn.html')">View co-detection of RNA and DNA data</a></small></div> </div> <div class="pdp-desc-summary"> <p>Reliance One-Step Multiplex Supermix is designed to efficiently detect both RNA and DNA targets in the same reaction. Whether samples are tested for both RNA and DNA viruses at the same time or are run with internal controls, Reliance One-Step Multiplex Supermix reliably amplifies multiple targets regardless of their nature.</p> <small><a class="documentation-pdf-icon no-target-style" href="/webroot/web/pdf/lsr/literature/Bulletin_7196.pdf">View Application Note</a> (PDF 143KB)</small></div> <div class="pdp-header"> <h2 class="overview_header">Streamline Your Workflow</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li><a href="../product/cell-lysis-rt-qpcr-kits?ID=NVENZGE8Z">SingleShot Cell Lysis Kit</a>, for the generation of lysates for use in qPCR or western blots</li> <li><a href="../product/primepcr-pcr-primers-assays-arrays?ID=M0HROA15">PrimePCR Probe Assays</a> for multiplexing gene expression targets with minimal cross-reactivity and maximum transcript coverage</li> <li><a href="../product/cfx-automation-system-ii?ID=KR44O415">CFX Automation System II</a> for walk-away high-throughput qPCR operation</li> </ul> <div class="pdp-header"> <h2 class="overview_header">4x Reliance One-Step Multiplex Supermix is Formulated with:</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li>Optimal blend of Reliance chimeric reverse transcriptase* and high-performance DNA polymerases including Sso7d fusion DNA polymerase**</li> <li>4X reaction buffer containing dNTPs, RNase inhibitor, and other reaction optimizing components</li> <li>Reaction stabilizers</li> <li>Patented*** universal reference dye</li> </ul> <div class="pdp-header"> <h2 class="overview_header">Applications and Uses</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li>Gene expression analysis</li> <li>Viral/pathogen detection&nbsp;(RNA viruses, DNA viruses, bacterial viruses, and fungal viruses)</li> <li>Diagnostic research</li> <li>High-throughput screening&nbsp;(small molecules, drug discovery, dosage experiment, CRISPR screening, and toxicology study)</li> </ul> <div class="pdp-header"> <h2 class="overview_header">Packaging Options</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="middle"><strong>Kit Size</strong></td> <td valign="middle"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="middle">1 ml (1 x 1 ml vial), 200 x 20 &mu;l reactions</td> <td valign="middle">12010176</td> </tr> <tr> <td valign="middle">5 ml (5 x 1 ml vial), 1,000 x 20 &mu;l reactions</td> <td valign="middle"><a href="/en-us/sku/12010220-reliance-one-step-multiplex-supermix-1000-x-20-ul-rxns-5-ml?ID=12010220">12010220</a></td> </tr> <tr class="pd_colorbackground"> <td valign="middle">10 ml (2 x 5 ml vial), 2,000 x 20 &mu;l reactions</td> <td valign="middle"><a href="/en-us/sku/12010221-reliance-one-step-multiplex-supermix-2000-x-20-ul-rxns-10-ml?ID=12010221">12010221</a></td> </tr> </tbody> </table> <div class="pdp-header"> <h2 class="overview_header" style="margin: 10px 0 0 10px; clear: both;">Instrument Compatibility</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <p>Reliance One-Step Multiplex Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td colspan="2" valign="middle"><strong>Systems</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">Applied Biosystems</td> <td valign="top"> <ul> <li>StepOne/StepOnePlus</li> <li>7500, ViiA 7</li> <li>7000, 7300, 7700, 7900HT, QuantStudio Systems</li> </ul> </td> </tr> <tr> <td valign="top">Stratagene</td> <td valign="top"> <ul> <li>Mx3000P, 3005P, 4000</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Eppendorf</td> <td valign="top"> <ul> <li>Mastercycler ep realplex 2, 4</li> </ul> </td> </tr> <tr> <td valign="top">QIAGEN</td> <td valign="top"> <ul> <li>Corbett Rotor-Gene 3000, 6000, Q</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Roche</td> <td valign="top"> <ul> <li>LightCycler 480, 96</li> <li>LightCycler 1.0, 1.5, 2.0</li> </ul> </td> </tr> </tbody> </table> <div class="pdp-header"> <h2 class="overview_header">Choose the Right One-Step RT qPCR Product for Your Needs</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td valign="top">&nbsp;</td> <td valign="top"><strong>Reliance One-Step Multiplex Supermix</strong></td> <td valign="top"><strong>iTaq Universal One-Step Probe Kit</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top"><strong>Key Features</strong></td> <td valign="top"> <ul> <li>Sensitive, efficient amplification of up to 5 targets</li> <li>Room temperature stable up to 24 hours</li> </ul> </td> <td valign="top"> <ul> <li>Reliable amplification of up to 2 targets</li> <li>Compatible with a fast protocol</li> </ul> </td> </tr> <tr> <td valign="top"><strong>Ideal for</strong></td> <td valign="top"> <ul> <li>Viral/pathogen detection in high-multiplex reaction</li> <li>High throughput screening</li> </ul> </td> <td valign="top"> <ul> <li>Viral/pathogen detection</li> <li>Singleplex and duplex reactions</li> </ul> </td> </tr> </tbody> </table> <div class="pdp-header"> <h2 class="overview_header">Related Products</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li><a href="../product/cell-lysis-rt-qpcr-kits?ID=NVENZGE8Z">Cell Lysis Kits</a></li> <li><a href="../category/rna-isolation?ID=2b86ef8e-07b5-4549-a544-0293620bb005">RNA Isolation Kits</a></li> <li><a href="../product/primepcr-pcr-primers-assays-arrays?ID=M0HROA15">PrimePCR Predesigned Probe qPCR Assays for Gene Expression</a> (Available in the U.S., Canada, E.U., China, Australia, New Zealand, Singapore, Taiwan, Hong Kong, Israel, South Africa, Thailand, Macau, Korea, and Japan.)</li> <li><a href="../product/cfx-automation-system-ii?ID=KR44O415">Real-Time PCR Automation System</a></li> </ul> <div class="pdp-header"> <h2 class="overview_header" style="margin: 10px 0 0 10px; clear: both;">Supporting Documents</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <ul> <li>Amplification Reagents and Plastics <a href="/webroot/web/pdf/lsr/literature/Bulletin_6090.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">Brochure</a></li> <li>SingleShot Cell Lysis Kit <a href="/webroot/web/pdf/lsr/literature/10000112808.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">Instruction Manual</a></li> <li>One-Step Multiplex RT-qPCR Using PrimePCR Probe Assays <a href="/webroot/web/pdf/lsr/literature/Bulletin_7197.pdf" class="no-target-style">Protocol</a></li> </ul> <div class="pdp-header"> <h2 class="overview_header" style="margin: 10px 0 0 10px; clear: both;">Online Resources</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <p><a href="/en-us/applications-technologies/what-real-time-pcr-qpcr?ID=LUSO4W8UU" target="_blank" rel="noopener noreferrer" class="new-target-image" title="Link opens in new window">What is Real-Time PCR (qPCR)?</a></p> <div class="pdp-header"> <h2 class="overview_header" style="margin: 10px 0 0 10px; clear: both;">More Information</h2> </div> <img style="margin: 5px 10px 15px;" src="/evportal/framework/skins/evolution/images/1x1grey.gif" border="0" alt="" width="620" height="1" /> <p>To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step <a href="/en-us/applications-technologies/qpcr-assay-design-optimization?ID=LUSO7RIVK">design and optimization protocols</a>. We also provide insights into analysis of <a href="/en-us/applications-technologies/real-time-pcr-data-analysis?ID=LUSOA9LPT">qPCR data</a> and <a href="/webroot/web/pdf/lsr/literature/10031339.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">troubleshooting reactions</a>.</p> <p>* Patent pending<br />** U.S. patents 6,627,424; 7,541,170; and 7,560,260.<br /> *** US patent 9,493,824</p> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global 12010176.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/12010176.html Product format 3184 Single-tube, ready-to use supermix 1 0 Concentration  1484 4x concentrated 1 0 Detection chemistry 3185 Probes 1 0 Multiplex capability 3183 Up to 5 targets 1 0 Assembled reaction stability 3181 Up to 24 hours 1 0 Inhibitor tolerance 3180 Tested with ethanol and hematin. Also compatible with up to 10 µl of crude lysates 1 0 5’ to 3’ exonuclease activity of the enzyme(s) 3182 Yes 1 0 Hot-start, activation time 3189 Yes, 10 min 1 0 Sample input 3179 1 pg to 1 µg 1 0 Sensitivity 2137 10 copies in a 5-plex reaction 1 0 Reference dye 3186 Tented universal dye preblended in the supermix 1 0 Instrument compatibility 2752 Both ROX-dependent (high- and low-) and -independent instruments 1 0 Guaranteed shelf life at –20° 3104 12 months 1 0 Shelf life at 4°C (after thawing) 3105 1 week 1 0 false €470.00 false null true false true true false EUR true true true /prd/en/FI/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12010176&quantity= false false /prd/en/FI/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12010176&CartQuote=QT&quantity= 1468 false true false /prd/en/FI/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12010176&AddToDefaultWishList=true&quantity= 41106300 470.00 SKU detail page 120-10176-MSDS Reliance One-Step Multiplex Supermix Australia English /webroot/web/pdf/WWMSDS/LSGC/AUS/AUS_ENG_12010176.pdf Korea Korean /webroot/web/pdf/WWMSDS/LSGC/ROK/ROK_KOR_12010176.pdf Belgium French /webroot/web/pdf/WWMSDS/LSGC/B/B_FR_12010176.pdf Italy Spanish /webroot/web/pdf/WWMSDS/LSGC/ISL/ISL_ES_12010176.pdf Mexico Spanish /webroot/web/pdf/WWMSDS/LSGC/MEX/MEX_ES_12010176.pdf Canada French /webroot/web/pdf/WWMSDS/LSGC/FCN/FCN_FR_12010176.pdf The Netherlands Dutch /webroot/web/pdf/WWMSDS/LSGC/NL/NL_NL_12010176.pdf Russian Federation Russian /webroot/web/pdf/WWMSDS/LSGC/RUS/RUS_RUS_12010176.pdf Hungary English /webroot/web/pdf/WWMSDS/LSGC/HE/HE_ENG_12010176.pdf Hungary Hungarian /webroot/web/pdf/WWMSDS/LSGC/H/H_UNG_12010176.pdf Brazil Portuguese /webroot/web/pdf/WWMSDS/LSGC/BRA/BRA_PT_12010176.pdf Germany German /webroot/web/pdf/WWMSDS/LSGC/D/D_D_12010176.pdf Czech Republic Czech /webroot/web/pdf/WWMSDS/LSGC/CZ/CZ_TS_12010176.pdf Denmark Danish /webroot/web/pdf/WWMSDS/LSGC/DK/DK_DA_12010176.pdf Denmark English /webroot/web/pdf/WWMSDS/LSGC/DKE/DKE_ENG_12010176.pdf Turkey Turkish /webroot/web/pdf/WWMSDS/LSGC/TR/TR_TR_12010176.pdf Germany English /webroot/web/pdf/WWMSDS/LSGC/DE/DE_ENG_12010176.pdf Poland Polish /webroot/web/pdf/WWMSDS/LSGC/PL/PL_POL_12010176.pdf Belgium English /webroot/web/pdf/WWMSDS/LSGC/BE/BE_ENG_12010176.pdf Hong Kong English /webroot/web/pdf/WWMSDS/LSGC/HK/HK_ENG_12010176.pdf Italy Italian /webroot/web/pdf/WWMSDS/LSGC/I/I_IT_12010176.pdf Portugal Portuguese /webroot/web/pdf/WWMSDS/LSGC/P/P_PT_12010176.pdf Ireland English /webroot/web/pdf/WWMSDS/LSGC/IE/IE_ENG_12010176.pdf Canada English /webroot/web/pdf/WWMSDS/LSGC/CDN/CDN_USA_12010176.pdf USA English /webroot/web/pdf/WWMSDS/LSGC/USA/USA_USA_12010176.pdf China Chinese /webroot/web/pdf/WWMSDS/LSGC/RC/RC_ZH_12010176.pdf Spain Spanish /webroot/web/pdf/WWMSDS/LSGC/E/E_ES_12010176.pdf Sweden Swedish /webroot/web/pdf/WWMSDS/LSGC/S/S_S_12010176.pdf Singapore English /webroot/web/pdf/WWMSDS/LSGC/SGP/SGP_ENG_12010176.pdf Czech Republic English /webroot/web/pdf/WWMSDS/LSGC/CZE/CZE_ENG_12010176.pdf United Kingdom English /webroot/web/pdf/WWMSDS/LSGC/GB/GB_ENG_12010176.pdf Austria English /webroot/web/pdf/WWMSDS/LSGC/AE/AE_ENG_12010176.pdf Sweden English /webroot/web/pdf/WWMSDS/LSGC/SE/SE_ENG_12010176.pdf France English /webroot/web/pdf/WWMSDS/LSGC/FE/FE_ENG_12010176.pdf New Zealand English /webroot/web/pdf/WWMSDS/LSGC/NZ/NZ_ENG_12010176.pdf Switzerland German /webroot/web/pdf/WWMSDS/LSGC/CH/CH_D_12010176.pdf China English /webroot/web/pdf/WWMSDS/LSGC/PRC/PRC_ENG_12010176.pdf India English /webroot/web/pdf/WWMSDS/LSGC/IN/IN_ENG_12010176.pdf Switzerland English /webroot/web/pdf/WWMSDS/LSGC/CHE/CHE_ENG_12010176.pdf Korea English /webroot/web/pdf/WWMSDS/LSGC/KOR/KOR_ENG_12010176.pdf Suomi Finnish /webroot/web/pdf/WWMSDS/LSGC/SF/SF_SF_12010176.pdf true Israel English /webroot/web/pdf/WWMSDS/LSGC/IL/IL_ENG_12010176.pdf Poland English /webroot/web/pdf/WWMSDS/LSGC/POL/POL_ENG_12010176.pdf France French /webroot/web/pdf/WWMSDS/LSGC/F/F_FR_12010176.pdf The Netherlands English /webroot/web/pdf/WWMSDS/LSGC/NLE/NLE_ENG_12010176.pdf Austria German /webroot/web/pdf/WWMSDS/LSGC/A/A_D_12010176.pdf Switzerland French /webroot/web/pdf/WWMSDS/LSGC/CHF/CHF_FR_12010176.pdf Norway Norwegian /webroot/web/pdf/WWMSDS/LSGC/N/N_N_12010176.pdf
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Use the Reliance One-Step Multiplex Supermix for sensitive amplification of up to 5 targets in a single reaction. Expertly formulated with high-performance enzymes and advanced reaction buffer, this robust supermix delivers reproducible RT-qPCR performance in a multiplex fashion, including with challenging templates, difficult targets or in the presence of PCR inhibitors. With its exceptional room temperature stability for assembled reactions, it can be easily integrated into an automated workflow. Reliance One-Step Multiplex Supermix is an ideal solution for multiplexed RT-qPCR, especially for pathogen/viral detection and high throughput screening applications.

Features and Benefits

Exceptional Performance of 5-target Multiplex

Reliance One-Step Multiplex Supermix enables researchers to get the most data out of their samples. Optimally formulated with a novel proprietary Reliance Reverse Transcriptase and robust DNA polymerases blend, this supermix amplifies up to 5 targets across a broad linear dynamic range from 1 pg to 1 µg of RNA input with maximum sensitivity and efficiency.

Unparalleled Room Temperature Stability for Up To 24 Hours

Reliance One-Step Multiplex Supermix is developed with high-throughput researchers in mind. Whether you want to queue up plates ahead of time or use an automated workflow, Reliance One-Step Multiplex Supermix will maintain its performance even after 24 hour incubation of assembled reactions.



Efficient Co-detection of RNA and DNA Targets

Reliance One-Step Multiplex Supermix is designed to efficiently detect both RNA and DNA targets in the same reaction. Whether samples are tested for both RNA and DNA viruses at the same time or are run with internal controls, Reliance One-Step Multiplex Supermix reliably amplifies multiple targets regardless of their nature.

View Application Note (PDF 143KB)

Streamline Your Workflow

4x Reliance One-Step Multiplex Supermix is Formulated with:

  • Optimal blend of Reliance chimeric reverse transcriptase* and high-performance DNA polymerases including Sso7d fusion DNA polymerase**
  • 4X reaction buffer containing dNTPs, RNase inhibitor, and other reaction optimizing components
  • Reaction stabilizers
  • Patented*** universal reference dye

Applications and Uses

  • Gene expression analysis
  • Viral/pathogen detection (RNA viruses, DNA viruses, bacterial viruses, and fungal viruses)
  • Diagnostic research
  • High-throughput screening (small molecules, drug discovery, dosage experiment, CRISPR screening, and toxicology study)

Packaging Options

Kit Size Catalog Number
1 ml (1 x 1 ml vial), 200 x 20 μl reactions 12010176
5 ml (5 x 1 ml vial), 1,000 x 20 μl reactions 12010220
10 ml (2 x 5 ml vial), 2,000 x 20 μl reactions 12010221

Instrument Compatibility

Reliance One-Step Multiplex Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:

Systems
Applied Biosystems
  • StepOne/StepOnePlus
  • 7500, ViiA 7
  • 7000, 7300, 7700, 7900HT, QuantStudio Systems
Stratagene
  • Mx3000P, 3005P, 4000
Eppendorf
  • Mastercycler ep realplex 2, 4
QIAGEN
  • Corbett Rotor-Gene 3000, 6000, Q
Roche
  • LightCycler 480, 96
  • LightCycler 1.0, 1.5, 2.0

Choose the Right One-Step RT qPCR Product for Your Needs

  Reliance One-Step Multiplex Supermix iTaq Universal One-Step Probe Kit
Key Features
  • Sensitive, efficient amplification of up to 5 targets
  • Room temperature stable up to 24 hours
  • Reliable amplification of up to 2 targets
  • Compatible with a fast protocol
Ideal for
  • Viral/pathogen detection in high-multiplex reaction
  • High throughput screening
  • Viral/pathogen detection
  • Singleplex and duplex reactions

Related Products

Supporting Documents

Online Resources

What is Real-Time PCR (qPCR)?

More Information

To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step design and optimization protocols. We also provide insights into analysis of qPCR data and troubleshooting reactions.

* Patent pending
** U.S. patents 6,627,424; 7,541,170; and 7,560,260.
*** US patent 9,493,824

Product format Single-tube, ready-to use supermix
Concentration  4x concentrated
Detection chemistry Probes
Multiplex capability Up to 5 targets
Assembled reaction stability Up to 24 hours
Inhibitor tolerance Tested with ethanol and hematin. Also compatible with up to 10 µl of crude lysates
5’ to 3’ exonuclease activity of the enzyme(s) Yes
Hot-start, activation time Yes, 10 min
Sample input 1 pg to 1 µg
Sensitivity 10 copies in a 5-plex reaction
Reference dye Tented universal dye preblended in the supermix
Instrument compatibility Both ROX-dependent (high- and low-) and -independent instruments
Guaranteed shelf life at –20° 12 months
Shelf life at 4°C (after thawing) 1 week
Number Description Options
Available Translation
Reliance One-Step Multiplex Supermix  (SDS)gene

Bulletin# 120-10176-MSDS

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