EveryBlot Blocking Buffer, 500 ml #12010020

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12010020 [x-forwarded-proto] = [https] [accept-language] = [en-US,en;q=0.5] [x-forwarded-port] = [443] [x-forwarded-for] = [35.168.112.145, 10.232.19.196] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [seourl] = [/en-fi/sku/12010020-everyblot-blocking-buffer-500-ml] [x-amzn-trace-id] = [Root=1-5f0a2cc2-ca1cce16549a90eccc9953fc] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=12010020] [host] = [10.232.1.21:1776] [x-request-uri] = [/en-fi/sku/12010020-everyblot-blocking-buffer-500-ml] [connection] = [Keep-Alive] [accept-encoding] = [br,gzip] [user-agent] = [CCBot/2.0 (https://commoncrawl.org/faq/)] Catalog/ProductSKU pageStyleKey 12010020-everyblot-blocking-buffer-500-ml LSR en FI en-fi /prd false 07/30/13 03:25 AM en_US true internet/generic 2020-01-17 15:39:20 /default/main/bio-rad/STAGING 1375179935592 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml page_EDUPriceOverlay /default/main/bio-rad/WORKAREA/default jddgmm0t 2017-11-14 06:29:46 true jddgmm0t templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml LSE N /default/main/bio-rad en 07/30/20 05:00 PM 1557002494330 2020-01-17 15:39:20 /default/main/bio-rad/WORKAREA/default/templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml edu-price-overlay 1 1375179935592 page_EDUPriceOverlay <div id="eduPriceOverlay" class="overlay" style="display: block;"> <p class="headerClassText">What is the education discount policy?</p> <p class="descClassText">For more than 20 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows K&ndash;12 and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices. These discounted products are indicated by an EDU suffix (for example, 1660003EDU).</p> <p class="headerClassText">How do I apply for the education discount?</p> <p class="descClassText">If you are an educator at the K&ndash;12 or college level, contact your local <a href="/en-au/contact-us">Customer Service</a> office to establish an education account number.</p> </div> 0 /webroot/web/html EDU_price_overlay.html /webroot/web/html/EDU_price_overlay.html sandeep Edu Edu Price Overlay Edu Price Overlay Content 07/30/13 03:25 AM 07/30/20 05:00 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE N 0 {"ecommerce":{"detail":{"products":{"price":"\u20ac125.00","name":"EveryBlot Blocking Buffer, 500 ml","variant":"BRC","id":"12010020","category":"BRC","brand":"BRC"}}},"event":"detail"} 12010020 08/27/19 11:40 PM en_US true internet/product_SKU 2020-02-18 22:28:01 /default/main/bio-rad/STAGING AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,VN,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/12010020_1566949953.xml /default/main/bio-rad/WORKAREA/default jzly4n8p 2019-08-27 23:52:33 true jzly4n8p templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/12010020_1566949953.xml LSR N /default/main/bio-rad en 09/02/29 10:39 AM 1575322046039 2020-02-18 22:28:01 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/12010020_1566949953.xml 12010020-everyblot-blocking-buffer-500-ml 1 12010020 EveryBlot Blocking Buffer 500 ml EveryBlot Blocking Buffer, 500 ml Bottle of blocking buffer, 500 ml. Requires only 5 minutes of blocking for all western blots Bottle of blocking buffer, 500 ml. Requires only 5 minutes of blocking for all western blots /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/12010020_th.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/12010020_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/12010020_view.jpg preview Jessica Low EveryBlot Blocking Buffer, 500 ml Bottle of blocking buffer, 500 ml. Requires only 5 minutes of blocking for all western blots blocking buffer, western blot, western blotting, immunoblot 08/27/19 11:40 PM 09/02/29 10:39 AM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,VN,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <div class="right"><center><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/electrophoresis/product_overlay/global/everyblot_video.html')"><img src="/webroot/web/images/lsr/products/electrophoresis/product_detail/global/yt-everyblot-th2.png" /></a> <p>A five minute blocking buffer for ALL western blots.</p> </center></div> <p class="wraptxt">EveryBlot Blocking Buffer provides 5 minute blocking and maximum sensitivity for all western blots regardless of detection method.</p> <h2 class="descHeader">Features and Benefits</h2> <ul> <li>Reduces non-specific antibody binding to reduce background while maintaining excellent sensitivity</li> <li>For both chemiluminescent and fluorescent detection</li> <li>Contains no phosphate-based buffers. Ideal for use with phospho-specific antibodies</li> <li>Complete blocking in 5 minutes</li> </ul> <a name="fluorescentdetection"></a> <div class="breakoutfeature"> <h2>Recommended Protocol</h2> <div class="corner-box bg-white"> <p>For mini-sized blots, use at least 10 ml for blocking and antibody incubation steps. For midi-sized blots, use at least 20 ml.</p> <ul> <li>Add block to the membrane and incubate for 5 minutes with agitation</li> <li>Dilute primary and secondary antibodies in full-strength block and incubate for 1 hour with agitation</li> </ul> <p>For fluorescent detection on PVDF, add SDS to 0.02% in the secondary antibody solution.</p> </div> </div> <a name="fluorescentdetection"></a> <h2 class="descHeader bttm-border">Fluorescent Detection</h2> <img src="/webroot/web/images/lsr/products/electrophoresis/product_detail/global/low-background-high-signal-th.png" alt="Clarity Western ECL Substrate" class="top-margin" /><br /> <p><strong>Fluorescent multiplex detection in HEK293 cells.</strong> After electrophoresis, proteins were transferred to low fluorescence PVDF. Left, blot blocked in a fluorescent optimized blocker for 1 hour. Right, blot blocked in EveryBlot Blocking Buffer for 5 minutes. After blocking, both blots processed identically and imaged to maximize signal and minimize background for each channel independently.</p> <div class="breakoutfeature bg-99ca3d30"> <h2 class="bttm-border">Chemiluminescent Detection</h2> <img src="/webroot/web/images/lsr/products/electrophoresis/product_detail/global/low-background-high-signal-chemiluminescence-th.png" alt="Clarity Max Western ECL Substrate" class="top-margin" /> <p><strong>Chemiluminescent detection of three targets in HEK 293 cells.</strong> After blocking, both blots processed identically and imaged and displayed using identical parameters.</p> </div> <h2 class="descHeader bttm-border">Phosphoprotein Detection</h2> <center><img src="/webroot/web/images/lsr/products/electrophoresis/product_detail/global/compatible-phospho-detection.png" alt="Compatibility" width="auto" height="auto" class="top-margin" /></center> <p><strong>Nothing to interfere with phosphoprotein detection. </strong>Detection of total (green) and phospho-specific (red) targets. Left, Jurkat cells unstimulated (&ndash;) or stimulated (+) with calyculin A. Center and right, A431 cells unstimulated (&ndash;) or stimulated (+) with EGF.</p> <!---<div style="background-color: #eeececc2; width: 650px;">---> <div class="breakoutfeature bg-ffcc0029"> <h2 class="bttm-border">Low Background across the Entire Spectrum</h2> <img src="/webroot/web/images/lsr/products/electrophoresis/product_detail/global/low-inherent-fluorescence-th.png " alt="Low inherent fluorescence" /> <p><strong>Effect of blocking agent on membrane background.</strong> Low fluorescence PVDF membranes without sample or antibodies blocked in either a fluorescence optimized blocking buffer or EveryBlot Blocking Buffer. After washing, membranes were imaged side by side on the ChemiDoc MP Imaging System. Membrane autofluorescence was measured in each fluorescent channel. Other vendor's buffer (<img style="width: 8px; height: 8px; background-color: #999999;" src="/webroot/web/images/1x1.gif" alt="" />); EveryBlot Buffer (<img style="width: 8px; height: 8px; background-color: #090;" src="/webroot/web/images/1x1.gif" alt="" />).</p> </div> <h2 class="descHeader">Packaging Options</h2> <table role="presentation" class="pd_table pd_gridlines-alternate"> <thead> <tr> <th scope="col"><span class="left">Product</span></th> <th scope="col">Catalog Number</th> </tr> </thead> <tbody> <tr> <td>EveryBlot Blocking Buffer, 50 ml</td> <td><a href="/en-us/sku/12010947-everyblot-blocking-buffer-50-ml?ID=12010947">12010947</a></td> </tr> <tr> <td>EveryBlot Blocking Buffer, 500 ml</td> <td>12010020</td> </tr> </tbody> </table> <h2 class="descHeader">Related Products</h2> <ul> <li>1x Tris Buffered Saline (TBS) with 1% Casein (<a href="/en-us/sku/1610782-1x-tris-buffered-saline-tbs-with-1-casein?ID=1610782">1610782</a>)</li> <li>1x Phosphate Buffered Saline (PBS) with 1% Casein (<a href="/en-us/sku/1610783-1x-phosphate-buffered-saline-pbs-with-1-casein?ID=1610783">1610783</a>)</li> <li>Blotting-Grade Blocker (<a href="/en-us/sku/1706404-blotting-grade-blocker?ID=1706404">1706404</a>)</li> <li>Gelatin (<a href="/en-us/sku/1706537-gelatin?ID=1706537">1706537</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/western-blotting?ID=9324fd3c-6af4-4551-831e-8db6fa3f3452">Western Blotting Membranes</a></li> <li><a href="/en-us/category/immunodetection-reagents-kits?ID=534c009d-cd86-4b33-956c-674d5a6ae054">Immunodetection Reagents and Kits</a></li> <li><a href="/en-us/category/western-blotting?ID=9324fd3c-6af4-4551-831e-8db6fa3f3452">Western Blotting Products</a></li> <li><a href="/en-us/category/protein-electrophoresis-blotting">Protein Electrophoresis and Blotting</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>EveryBlot Blocking Buffer <a href="/webroot/web/pdf/lsr/literature/Bulletin_7185.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">Flier</a></li> </ul> /webroot/web/html/lsr/products/electrophoresis/sku_view/description/global 12010020.html /webroot/web/html/lsr/products/electrophoresis/sku_view/description/global/12010020.html Storage temperature 2212 4°C 1 0 Shelf life 2149 At least 6 months 1 0 Detection method 2749 Colorimetric, chemiluminescence, fluorescence 1 0 Notes 2814 * low backround and high signal on fluorescent and chemiluminescent blots</br> * Compatible with phosphorylated protein detection</br> * Complete blocking in 5 minutes</br> 1 0 7185 templatedata/internet/documentation/data/LSR/Literature/7185_1569886526.xml EveryBlot Blocking Buffer Flier 7185 /webroot/web/pdf/lsr/literature/Bulletin_7185.pdf Literature PDF Other /webroot/web/images/icon_pdf.gif EveryBlot Blocking Buffer Flier No EveryBlot Blocking Buffer Flier 7185 everyblot blocking buffer, fluorescent blocking buffer, western blocking buffer, Bulletin 7185, 12010020, 12010947 Jessica Low EveryBlot Blocking Buffer Flier EveryBlot Blocking Buffer used for western blots. EveryBlot blocking buffer, fluorescent blocking buffer, western blocking buffer, Bulletin 7185, 12010020, 12010947 09/30/19 11:28 PM 09/30/37 01:32 PM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,VN,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE LSR /LSR/Literature N 0 /prd/en/FI/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7185&quantity= 1706516 templatedata/internet/product_SKU/data/ja_JP/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6516_0921120403104.xml 1706515 templatedata/internet/product_SKU/data/ja_JP/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6515_0921120403177.xml 1705060 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Bottle of blocking buffer, 500 ml. Requires only 5 minutes of blocking for all western blots

12010020 [x-forwarded-proto] = [https] [accept-language] = [en-US,en;q=0.5] [x-forwarded-port] = [443] [x-forwarded-for] = [35.168.112.145, 10.232.19.196] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [seourl] = [/en-fi/sku/12010020-everyblot-blocking-buffer-500-ml] [x-amzn-trace-id] = [Root=1-5f0a2cc2-ca1cce16549a90eccc9953fc] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=12010020] [host] = [10.232.1.21:1776] [x-request-uri] = [/en-fi/sku/12010020-everyblot-blocking-buffer-500-ml] [connection] = [Keep-Alive] [accept-encoding] = [br,gzip] [user-agent] = [CCBot/2.0 (https://commoncrawl.org/faq/)] Catalog/ProductSKU pageStyleKey 12010020-everyblot-blocking-buffer-500-ml LSR en FI en-fi /prd false 07/30/13 03:25 AM en_US true internet/generic 2020-01-17 15:39:20 /default/main/bio-rad/STAGING 1375179935592 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml page_EDUPriceOverlay /default/main/bio-rad/WORKAREA/default jddgmm0t 2017-11-14 06:29:46 true jddgmm0t templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml LSE N /default/main/bio-rad en 07/30/20 05:00 PM 1557002494330 2020-01-17 15:39:20 /default/main/bio-rad/WORKAREA/default/templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml edu-price-overlay 1 1375179935592 page_EDUPriceOverlay <div id="eduPriceOverlay" class="overlay" style="display: block;"> <p class="headerClassText">What is the education discount policy?</p> <p class="descClassText">For more than 20 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows K&ndash;12 and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices. These discounted products are indicated by an EDU suffix (for example, 1660003EDU).</p> <p class="headerClassText">How do I apply for the education discount?</p> <p class="descClassText">If you are an educator at the K&ndash;12 or college level, contact your local <a href="/en-au/contact-us">Customer Service</a> office to establish an education account number.</p> </div> 0 /webroot/web/html EDU_price_overlay.html /webroot/web/html/EDU_price_overlay.html sandeep Edu Edu Price Overlay Edu Price Overlay Content 07/30/13 03:25 AM 07/30/20 05:00 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE N 0 {"ecommerce":{"detail":{"products":{"price":"\u20ac125.00","name":"EveryBlot Blocking Buffer, 500 ml","variant":"BRC","id":"12010020","category":"BRC","brand":"BRC"}}},"event":"detail"} 12010020 08/27/19 11:40 PM en_US true internet/product_SKU 2020-02-18 22:28:01 /default/main/bio-rad/STAGING AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,VN,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/12010020_1566949953.xml /default/main/bio-rad/WORKAREA/default jzly4n8p 2019-08-27 23:52:33 true jzly4n8p templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/12010020_1566949953.xml LSR N /default/main/bio-rad en 09/02/29 10:39 AM 1575322046039 2020-02-18 22:28:01 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/12010020_1566949953.xml 12010020-everyblot-blocking-buffer-500-ml 1 12010020 EveryBlot Blocking Buffer 500 ml EveryBlot Blocking Buffer, 500 ml Bottle of blocking buffer, 500 ml. Requires only 5 minutes of blocking for all western blots Bottle of blocking buffer, 500 ml. Requires only 5 minutes of blocking for all western blots /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/12010020_th.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/12010020_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/12010020_view.jpg preview Jessica Low EveryBlot Blocking Buffer, 500 ml Bottle of blocking buffer, 500 ml. Requires only 5 minutes of blocking for all western blots blocking buffer, western blot, western blotting, immunoblot 08/27/19 11:40 PM 09/02/29 10:39 AM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,VN,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <div class="right"><center><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/electrophoresis/product_overlay/global/everyblot_video.html')"><img src="/webroot/web/images/lsr/products/electrophoresis/product_detail/global/yt-everyblot-th2.png" /></a> <p>A five minute blocking buffer for ALL western blots.</p> </center></div> <p class="wraptxt">EveryBlot Blocking Buffer provides 5 minute blocking and maximum sensitivity for all western blots regardless of detection method.</p> <h2 class="descHeader">Features and Benefits</h2> <ul> <li>Reduces non-specific antibody binding to reduce background while maintaining excellent sensitivity</li> <li>For both chemiluminescent and fluorescent detection</li> <li>Contains no phosphate-based buffers. Ideal for use with phospho-specific antibodies</li> <li>Complete blocking in 5 minutes</li> </ul> <a name="fluorescentdetection"></a> <div class="breakoutfeature"> <h2>Recommended Protocol</h2> <div class="corner-box bg-white"> <p>For mini-sized blots, use at least 10 ml for blocking and antibody incubation steps. For midi-sized blots, use at least 20 ml.</p> <ul> <li>Add block to the membrane and incubate for 5 minutes with agitation</li> <li>Dilute primary and secondary antibodies in full-strength block and incubate for 1 hour with agitation</li> </ul> <p>For fluorescent detection on PVDF, add SDS to 0.02% in the secondary antibody solution.</p> </div> </div> <a name="fluorescentdetection"></a> <h2 class="descHeader bttm-border">Fluorescent Detection</h2> <img src="/webroot/web/images/lsr/products/electrophoresis/product_detail/global/low-background-high-signal-th.png" alt="Clarity Western ECL Substrate" class="top-margin" /><br /> <p><strong>Fluorescent multiplex detection in HEK293 cells.</strong> After electrophoresis, proteins were transferred to low fluorescence PVDF. Left, blot blocked in a fluorescent optimized blocker for 1 hour. Right, blot blocked in EveryBlot Blocking Buffer for 5 minutes. After blocking, both blots processed identically and imaged to maximize signal and minimize background for each channel independently.</p> <div class="breakoutfeature bg-99ca3d30"> <h2 class="bttm-border">Chemiluminescent Detection</h2> <img src="/webroot/web/images/lsr/products/electrophoresis/product_detail/global/low-background-high-signal-chemiluminescence-th.png" alt="Clarity Max Western ECL Substrate" class="top-margin" /> <p><strong>Chemiluminescent detection of three targets in HEK 293 cells.</strong> After blocking, both blots processed identically and imaged and displayed using identical parameters.</p> </div> <h2 class="descHeader bttm-border">Phosphoprotein Detection</h2> <center><img src="/webroot/web/images/lsr/products/electrophoresis/product_detail/global/compatible-phospho-detection.png" alt="Compatibility" width="auto" height="auto" class="top-margin" /></center> <p><strong>Nothing to interfere with phosphoprotein detection. </strong>Detection of total (green) and phospho-specific (red) targets. Left, Jurkat cells unstimulated (&ndash;) or stimulated (+) with calyculin A. Center and right, A431 cells unstimulated (&ndash;) or stimulated (+) with EGF.</p> <!---<div style="background-color: #eeececc2; width: 650px;">---> <div class="breakoutfeature bg-ffcc0029"> <h2 class="bttm-border">Low Background across the Entire Spectrum</h2> <img src="/webroot/web/images/lsr/products/electrophoresis/product_detail/global/low-inherent-fluorescence-th.png " alt="Low inherent fluorescence" /> <p><strong>Effect of blocking agent on membrane background.</strong> Low fluorescence PVDF membranes without sample or antibodies blocked in either a fluorescence optimized blocking buffer or EveryBlot Blocking Buffer. After washing, membranes were imaged side by side on the ChemiDoc MP Imaging System. Membrane autofluorescence was measured in each fluorescent channel. Other vendor's buffer (<img style="width: 8px; height: 8px; background-color: #999999;" src="/webroot/web/images/1x1.gif" alt="" />); EveryBlot Buffer (<img style="width: 8px; height: 8px; background-color: #090;" src="/webroot/web/images/1x1.gif" alt="" />).</p> </div> <h2 class="descHeader">Packaging Options</h2> <table role="presentation" class="pd_table pd_gridlines-alternate"> <thead> <tr> <th scope="col"><span class="left">Product</span></th> <th scope="col">Catalog Number</th> </tr> </thead> <tbody> <tr> <td>EveryBlot Blocking Buffer, 50 ml</td> <td><a href="/en-us/sku/12010947-everyblot-blocking-buffer-50-ml?ID=12010947">12010947</a></td> </tr> <tr> <td>EveryBlot Blocking Buffer, 500 ml</td> <td>12010020</td> </tr> </tbody> </table> <h2 class="descHeader">Related Products</h2> <ul> <li>1x Tris Buffered Saline (TBS) with 1% Casein (<a href="/en-us/sku/1610782-1x-tris-buffered-saline-tbs-with-1-casein?ID=1610782">1610782</a>)</li> <li>1x Phosphate Buffered Saline (PBS) with 1% Casein (<a href="/en-us/sku/1610783-1x-phosphate-buffered-saline-pbs-with-1-casein?ID=1610783">1610783</a>)</li> <li>Blotting-Grade Blocker (<a href="/en-us/sku/1706404-blotting-grade-blocker?ID=1706404">1706404</a>)</li> <li>Gelatin (<a href="/en-us/sku/1706537-gelatin?ID=1706537">1706537</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/western-blotting?ID=9324fd3c-6af4-4551-831e-8db6fa3f3452">Western Blotting Membranes</a></li> <li><a href="/en-us/category/immunodetection-reagents-kits?ID=534c009d-cd86-4b33-956c-674d5a6ae054">Immunodetection Reagents and Kits</a></li> <li><a href="/en-us/category/western-blotting?ID=9324fd3c-6af4-4551-831e-8db6fa3f3452">Western Blotting Products</a></li> <li><a href="/en-us/category/protein-electrophoresis-blotting">Protein Electrophoresis and Blotting</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>EveryBlot Blocking Buffer <a href="/webroot/web/pdf/lsr/literature/Bulletin_7185.pdf" target="_blank" rel="noopener noreferrer" class="no-target-style">Flier</a></li> </ul> /webroot/web/html/lsr/products/electrophoresis/sku_view/description/global 12010020.html /webroot/web/html/lsr/products/electrophoresis/sku_view/description/global/12010020.html Storage temperature 2212 4°C 1 0 Shelf life 2149 At least 6 months 1 0 Detection method 2749 Colorimetric, chemiluminescence, fluorescence 1 0 Notes 2814 * low backround and high signal on fluorescent and chemiluminescent blots</br> * Compatible with phosphorylated protein detection</br> * Complete blocking in 5 minutes</br> 1 0 7185 templatedata/internet/documentation/data/LSR/Literature/7185_1569886526.xml EveryBlot Blocking Buffer Flier 7185 /webroot/web/pdf/lsr/literature/Bulletin_7185.pdf Literature PDF Other /webroot/web/images/icon_pdf.gif EveryBlot Blocking Buffer Flier No EveryBlot Blocking Buffer Flier 7185 everyblot blocking buffer, fluorescent blocking buffer, western blocking buffer, Bulletin 7185, 12010020, 12010947 Jessica Low EveryBlot Blocking Buffer Flier EveryBlot Blocking Buffer used for western blots. EveryBlot blocking buffer, fluorescent blocking buffer, western blocking buffer, Bulletin 7185, 12010020, 12010947 09/30/19 11:28 PM 09/30/37 01:32 PM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,VN,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE LSR /LSR/Literature N 0 /prd/en/FI/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7185&quantity= 1706516 templatedata/internet/product_SKU/data/ja_JP/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6516_0921120403104.xml 1706515 templatedata/internet/product_SKU/data/ja_JP/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6515_0921120403177.xml 1705060 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A five minute blocking buffer for ALL western blots.

EveryBlot Blocking Buffer provides 5 minute blocking and maximum sensitivity for all western blots regardless of detection method.

Features and Benefits

  • Reduces non-specific antibody binding to reduce background while maintaining excellent sensitivity
  • For both chemiluminescent and fluorescent detection
  • Contains no phosphate-based buffers. Ideal for use with phospho-specific antibodies
  • Complete blocking in 5 minutes

Recommended Protocol

For mini-sized blots, use at least 10 ml for blocking and antibody incubation steps. For midi-sized blots, use at least 20 ml.

  • Add block to the membrane and incubate for 5 minutes with agitation
  • Dilute primary and secondary antibodies in full-strength block and incubate for 1 hour with agitation

For fluorescent detection on PVDF, add SDS to 0.02% in the secondary antibody solution.

Fluorescent Detection

Clarity Western ECL Substrate

Fluorescent multiplex detection in HEK293 cells. After electrophoresis, proteins were transferred to low fluorescence PVDF. Left, blot blocked in a fluorescent optimized blocker for 1 hour. Right, blot blocked in EveryBlot Blocking Buffer for 5 minutes. After blocking, both blots processed identically and imaged to maximize signal and minimize background for each channel independently.

Chemiluminescent Detection

Clarity Max Western ECL Substrate

Chemiluminescent detection of three targets in HEK 293 cells. After blocking, both blots processed identically and imaged and displayed using identical parameters.

Phosphoprotein Detection

Compatibility

Nothing to interfere with phosphoprotein detection. Detection of total (green) and phospho-specific (red) targets. Left, Jurkat cells unstimulated (–) or stimulated (+) with calyculin A. Center and right, A431 cells unstimulated (–) or stimulated (+) with EGF.

Low Background across the Entire Spectrum

Low inherent fluorescence

Effect of blocking agent on membrane background. Low fluorescence PVDF membranes without sample or antibodies blocked in either a fluorescence optimized blocking buffer or EveryBlot Blocking Buffer. After washing, membranes were imaged side by side on the ChemiDoc MP Imaging System. Membrane autofluorescence was measured in each fluorescent channel. Other vendor's buffer (); EveryBlot Buffer ().

Packaging Options

Related Products

  • 1x Tris Buffered Saline (TBS) with 1% Casein (1610782)
  • 1x Phosphate Buffered Saline (PBS) with 1% Casein (1610783)
  • Blotting-Grade Blocker (1706404)
  • Gelatin (1706537)

Related Categories

Supporting Documents

  • EveryBlot Blocking Buffer Flier
Storage temperature 4°C
Shelf life At least 6 months
Detection method Colorimetric, chemiluminescence, fluorescence
Notes * low backround and high signal on fluorescent and chemiluminescent blots
* Compatible with phosphorylated protein detection
* Complete blocking in 5 minutes
Number Description Options
7185 EveryBlot Blocking Buffer Flier Click to download
Available Translation
EveryBlot Blocking Buffer  (SDS)gene

Bulletin# 120-10020-MSDS

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