iTaq™ Fast Supermix With ROX

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Informazioni Generali

Two Target Expression
Two Target Expression

iTaq™ fast supermix with ROX delivers superior results for gene expression analysis of two targets on the ABI 7500 fast real-time PCR system, with no difference in detection of a low-expressing gene in duplex or singleplex. A 0.1 µg cDNA synthesis reaction of human liver total RNA was used in each 20 µl reaction. FAM-labeled β-actin probe duplex reaction (), VIC-labeled IL-1β probe duplex reaction (), FAM-labeled β-actin probe singleplex reaction (), VIC-labeled IL-1β probe singleplex reaction (). Total qPCR run time = 38 min.

Robust Duplex qPCR
Robust Duplex qPCR

Robust duplex qPCR results with iTaq™ fast supermix with ROX on the ABI 7500 fast real-time PCR system. cDNA inputs: 100-fold serial dilutions of cDNA from the equivalent of 1 µg to 1 pg total RNA were used in each 20 µl reaction. FAM-labeled 18S RNA probe duplex reaction (), VIC-labeled beta-2-microglobulin (B2M) probe duplex reaction (), VIC-labeled B2M probe singleplex reaction (). 18S efficiency = 98.6%, r = 0.999; B2M efficiency = 98.0%, r = 0.998. Total qPCR run time = 45 min.

iTaq fast supermix with ROX delivers maximum efficiency, sensitivity, and specificity and a robust fluorescent signal under fast or standard thermal cycling conditions with any real-time detection chemistry. Designed specifically for use on the ABI 7500 fast or standard real-time PCR system and the Stratagene real-time PCR systems, this supermix is preblended with iTaq DNA polymerase, optimized buffer, nucleotides, and ROX passive reference dye in a convenient one-tube formulation.

 

  • Robust, simultaneous detection of up to 2 different gene targets using fluorogenic probes under fast qPCR conditions
  • Rapid activation and polymerization kinetics for fast qPCR results in less than 40 min
  • Reproducible, highly uniform results across a wide range of template concentrations

 

Storage at –20°C Guaranteed for 12 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)
Storage at +4°C Guaranteed for 6 months at 4°C (protected from light)
iTaq Fast Supermix With ROX

172-5105
200 x 20 µl reactions, real-time PCR mix for use on ROX-dependent optical thermal cyclers, 2x mix contains dNTPs, iTaq DNA polymerase, 6 mM Mg2+, ROX reference dye, stabilizers

Prezzo di listino:   €160,00
Quantità:   Aggiungi a carrello
elenco preferiti   offerta
iTaq Fast Supermix With ROX

172-5106
500 x 20 µl reactions, real-time PCR mix for use on ROX-dependent optical thermal cyclers, 2x mix contains dNTPs, iTaq DNA polymerase, 6 mM Mg2+, ROX reference dye, stabilizers

Prezzo di listino:   €340,00
Quantità:   Aggiungi a carrello
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iTaq Fast Supermix With ROX

172-5107
1,000 x 20 µl reactions, real-time PCR mix for use on ROX-dependent optical thermal cyclers, 2x mix contains dNTPs, iTaq DNA polymerase, 6 mM Mg2+, ROX reference dye, stabilizers

Prezzo di listino:   €655,00
Quantità:   Aggiungi a carrello
elenco preferiti   offerta
iTaq Fast Supermix With ROX

172-5108
20 ml, 2,000 x 20 µl reactions, real-time PCR mix for use on ROX-dependent optical thermal cyclers, 2x mix contains dNTPs, iTaq DNA polymerase, 6 mM Mg2+, ROX reference dye, stabilizers

Prezzo di listino:   €1.200,00
Quantità:   Aggiungi a carrello
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Letteratura
Number Descrizione Opzioni
6090 Amplification Reagents and Plastics Brochure, Rev C Click to download [ Aggiungi a carrello ]
10013404 Instruction Manual, iTaq Fast Supermix With ROX, Rev A Click to download
5737 iTaq Fast Supermixes With ROX for qPCR Flier, Rev B Click to download [ Aggiungi a carrello ]
5345 Reagents for Reverse Transcription, PCR, and Real-Time PCR Brochure, Rev C Click to download
MSDS
Number Descrizione Opzioni
172-5105-MSDS iTaq Fast Supermix with Rox (English) Click to download
172-5105-MSDS iTaq Fast Supermix with Rox (Italian) Click to download
172-5105-MSDS iTaq Fast Supermix with Rox (Spanish) Click to download