iTaq™ Fast SYBR® Green Supermix With ROX

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Informazioni Generali

Linear Results
Linear Results

iTaq™ fast SYBR® Green supermix with ROX generates linear results over six orders of magnitude on the ABI 7500 fast real-time PCR system. Tenfold serial dilutions of 100 ng to 100 fg of cDNA from HeLa total RNA were used in each 20 µl reaction designed to detect β-actin. Efficiency = 93.0%, r = 0.998. Total qPCR run time = 31 min.

Magnitude
Magnitude

iTaq™ fast SYBR® Green supermix with ROX generates linear results over six orders of magnitude on the ABI 7500 fast real-time PCR system. Tenfold serial dilutions of 100 ng to 100 fg of cDNA from HeLa total RNA were used in each 20 µl reaction designed to detect 18S RNA. 18S efficiency = 95.9%, r = 1.000. Total qPCR run time = 30 min.

Fast or Standard
Fast or Standard

iTaq™ fast SYBR® Green supermix with ROX performs well with fast or standard cycling protocols. Tenfold serial dilutions of 100 ng to 100 fg of cDNA from HeLa total RNA were used in each 20 µl reaction designed to detect GAPDH. Fast cycling run time = 31 min; standard cycling run time = 74 min.

iTaq™ Fast SYBR® Green supermix with ROX is a 2x ready-to-use reaction mix formulated to deliver maximum efficiency, sensitivity, and specificity and a robust fluorescent signal under fast or standard thermal cycling conditions. Designed specifically for use on the ABI 7500 fast or standard real-time PCR system and Stratagene real-time PCR systems, this supermix is preblended with iTaq DNA polymerase, optimized buffer, nucleotides, SYBR® Green I dye, and ROX passive reference dye for normalization of fluorescent signal and to correct for well-to-well optical variations.

Key Features and Benefits

  • Effective across a broad range of concentrations — data illustrate linear results over six orders of magnitude for detecting gene targets with high sensitivity
  • Versatile and fast performance — reproducible, highly uniform results across a wide range of template concentrations under both fast and standard cycling protocols. The rapid activation and polymerization kinetics permit fast qPCR results in less than 40 min
  • Simple protocol — convenient one-tube formulations enable you to obtain specific, sensitive, and fast qPCR results with ease

Applications and Uses

  • PCR and real-time PCR analysis
  • Nucleic acid amplification and expression profiling
  • ROX-dependent real-time PCR detection
  • Gene expression analysis
  • Genotyping studies
  • Genomics-related applications
Storage at –20°C Guaranteed for 6 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)
Storage at +4°C Guaranteed for 3 months at 4°C (protected from light)
iTaq Fast SYBR Green Supermix With ROX

172-5100
200 x 20 µl reactions, real-time PCR mix for use on ROX-dependent optical thermal cyclers, 2x mix contains dNTPs, iTaq DNA polymerase, 6 mM Mg2+, SYBR Green I, ROX reference dye, stabilizers

Prezzo di listino:   €160,00
Quantità:   Aggiungi a carrello
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iTaq Fast SYBR Green Supermix With ROX

172-5101
500 x 20 µl reactions, real-time PCR mix for use on ROX-dependent optical thermal cyclers, 2x mix contains dNTPs, iTaq DNA polymerase, 6 mM Mg2+, SYBR Green I, ROX reference dye, stabilizers

Prezzo di listino:   €340,00
Quantità:   Aggiungi a carrello
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iTaq Fast SYBR Green Supermix With ROX

172-5102
1,000 x 20 µl reactions, real-time PCR mix for use on ROX-dependent optical thermal cyclers, 2x mix contains dNTPs, iTaq DNA polymerase, 6 mM Mg2+, SYBR Green I, ROX reference dye, stabilizers

Prezzo di listino:   €655,00
Quantità:   Aggiungi a carrello
elenco preferiti   offerta
iTaq Fast SYBR Green Supermix With ROX

172-5103
20 ml, 2,000 x 20 µl reactions, real-time PCR mix for use on ROX-dependent optical thermal cyclers, 2x mix contains dNTPs, iTaq DNA polymerase, 6 mM Mg2+, SYBR Green I, ROX reference dye, stabilizers

Prezzo di listino:   €1.200,00
Quantità:   Aggiungi a carrello
elenco preferiti   offerta
Letteratura
Number Descrizione Opzioni
6090 Amplification Reagents and Plastics Brochure, Rev C Click to download [ Aggiungi a carrello ]
10013409 Instruction Manual, iTaq Fast SYBR Green Supermix With ROX, Rev A Click to download
5737 iTaq Fast Supermixes With ROX for qPCR Flier, Rev B Click to download [ Aggiungi a carrello ]
5345 Reagents for Reverse Transcription, PCR, and Real-Time PCR Brochure, Rev C Click to download
MSDS
Number Descrizione Opzioni
172-5100-MSDS iTaq Fast SYBR Green Supermix with ROX (English) Click to download
172-5100-MSDS iTaq Fast SYBR Green Supermix with ROX (Italian) Click to download
172-5100-MSDS iTaq Fast SYBR Green Supermix with ROX (Spanish) Click to download