iTaq™ SYBR^® Green supermix with ROX is a 2x ready-to-use reaction mix, formulated to deliver maximum PCR efficiency and sensitivity and a robust fluorescent signal in real-time qPCR assays. This formula yields high performance with cDNA as well as genomic and plasmid DNA templates over a broad dynamic range, achieving sensitive and specific amplification over at least six orders of magnitude. This supermix is preblended with hot-start iTaq DNA polymerase, a proprietary buffer and stabilizer, nucleotides, an optimized SYBR^® Green I dye that minimizes the potential for primer-dimer formation, and ROX passive reference dye to correct for interwell signal variation. It has been validated for use on all ROX-dependent optical thermal cyclers and with any real-time detection chemistry.

Key Features and Benefits

• High specificity and convenience — the antibody-mediated, hot-start iTaq DNA polymerase enables specific and efficient primer extension with the convenience of room temperature reaction assembly
• Simple protocol — convenient 1-tube formulation, preblended with optimized chemistries to ensure sensitive and accurate detection of low-abundance targets
• Effective across a broad range of concentrations — data illustrate excellent performance over several orders of magnitude
• Reliable and uniform results — data show consistency in generating uniform results with earlier threshold cycle (C_T) values than other ROX-containing supermixes

Applications and Uses

• PCR and real-time PCR analysis
• Nucleic acid amplification and expression profiling
• ROX-dependent real-time PCR detection
• Gene expression analysis
• Genotyping studies
• Genomics-related applications