Biomarker Discovery Using SELDI Technology

Proper study and experimental design coupled with the correct data analysis approach are critical components of successful biomarker discovery, no matter which technology, platform, or workflow is used. Bio-Rad has produced two in-depth guides to provide researchers with strategies for success.

The first guide, Biomarker Discovery Using SELDI Technology — A Guide to Successful Study and Experimental Design, provides a series of general guidelines for effective study design using virtually any proteomics technology, and gives specific recommendations for using the ProteinChip surface-enhanced laser desorption/ionization (SELDI) system. This guide also includes:

Guidelines for proper sample selection, collection, handling, and storage Instructions for planning the experimental workflow Tips on improving reproducibility through standard operating procedures Important considerations for data analysis implementation Optimized methods and protocols for serum or plasma analysis

The second guide, Biomarker Discovery Using SELDI Technology — A Guide to Data Processing and Analysis Using ProteinChip Data Manager Software, is a comprehensive resource for data analysis workflows, guidelines, and specific recommendations for analyzing biomarker discovery data. The guide covers:

General data analysis workflows Data organization Data processing steps, including baseline subtraction, normalization, and spectrum alignment Statistical analysis using P values and ROC curves, hierarchical clustering, and principal component analysis Glossary of software terms