SsoAdvanced™ SYBR® Green Supermix #172-5265

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10 x 5 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, stabilizers, for 5,000 x 20 µl reactions

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Number Description Options
172-5260-MSDS SsoAdvanced™ SYBR® Green Supermix English

Packaging Options

  • Catalog #172-5260, 2 x 1 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, stabilizers, for 200 x 20 µl reactions
  • Catalog #172-5261, 5 x 1 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, stabilizers, for 500 x 20 µl reactions
  • Catalog #172-5262, 10 x 1 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, stabilizers, for 1,000 x 20 µl reactions
  • Catalog #172-5264, 5 x 5 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, stabilizers, for 2,500 x 20 µl reactions
  • Catalog #172-5265, 10 x 5 ml, 2x real-time PCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, stabilizers, for 5,000 x 20 µl reactions

SsoAdvanced SYBR Green supermix is the newest high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein technology. It is formulated for a wide range of qPCR applications. The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, providing superior inhibitor tolerance, increased processivity, and greater speed without affecting PCR sensitivity, efficiency, or reproducibility.

Key Features and Benefits

  • Achieve superior gene expression results under various cycling conditions — robust formulation delivers consistent performance in standard or fast cycling
  • Increase sensitivity and efficiency of detection from compromised samples — Sso7d fusion polymerase has increased resistance to a wide variety of PCR inhibitors
  • Decrease run times and time to results without compromising qPCR data quality — Sso7d fusion polymerase and optimized buffer provide rapid polymerization kinetics and instant polymerase activation
  • Obtain better results with predeveloped qPCR assays — advanced formulation tolerates a broad range of reaction conditions, primer concentrations, and temperature ranges

* U.S. patents 6,627,424; 7,541,170; and 7,560,260.