iTaq™ Universal SYBR® Green Supermix #172-5120

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2 ml (2 x 1 ml), 2x qPCR mix, contains dNTPs, iTaq DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, ROX normalization dyes, for 200 x 20 µl reactions

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Packaging Options

Catalog #172-5120, 2 ml (2 x 1 ml vials), 200 x 20 µl reactions, 2x qPCR mix, contains dNTPs, antibody-mediated hot-start iTaq DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, ROX normalization dyes
Catalog #172-5121, 5 ml (5 x 1 ml vials), 500 x 20 µl reactions, 2x qPCR mix, contains dNTPs, antibody-mediated hot-start iTaq DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, ROX normalization dyes
Catalog #172-5122, 10 ml (10 x 1 ml vials), 1,000 x 20 µl reactions, 2x qPCR mix, contains dNTPs, antibody-mediated hot-start iTaq DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, ROX normalization dyes
Catalog #172-5124, 25 ml (5 x 5 ml vials), 2,500 x 20 µl reactions, 2x qPCR mix, contains dNTPs, antibody-mediated hot-start iTaq DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, ROX normalization dyes
Catalog #172-5125, 50 ml (10 x 5 ml vials), 5,000 x 20 µl reactions, 2x qPCR mix, contains dNTPs, antibody-mediated hot-start iTaq DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, ROX normalization dyes

iTaq™ universal SYBR® Green supermix is a 2x concentrated, ready-to-use reaction master mix optimized for dye-based quantitative PCR (qPCR) on any real-time PCR instrument. It contains antibody-mediated hot-start iTaq DNA polymerase, dNTPs, MgCl2, SYBR® Green I dye, enhancers, stabilizers, and a blend of passive reference dyes (including ROX and fluorescein).

iTaq™ universal SYBR® Green supermix lets you:

iTaq™ universal SYBR® Green supermix contains:

  • Advanced buffer formulation with SYBR® Green I for optimal qPCR performance
  • Antibody-mediated hot-start iTaq DNA polymerase for rapid activation (30 sec)
  • Blend of passive reference dyes for universal real-time instrument compatibility

Applications and Uses

  • Gene expression analysis
  • Genotyping
  • Mutation detection
  • Pathogen detection