UNOsphere SUPrA Media

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Overview

mAb Workflow
Monoclonal Antibody Purification Workflow
Monoclonal Antibody Purification Workflow

Workflow for mAb purification with UNOsphere SUPra affinity, UNOsphere Q and CHT type I media.

PDF Bulletin 5728 To read more click here

Flow Performance
Flow Performance of UNOsphere SUPrA Media

Narrow elution profile of antibody on UNOsphere SUPrA media.

Cartridge Selection by Application
Prepacked Cartridge Selection by Application
Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml*
Protein and Plasmid Purification        
High Q Macro-Prep High Q Strong anion exchange -N+(CH3)3 ≥37 mg BSA
UNOsphere Q Unosphere Q Strong anion exchange -N+(CH3)3 180 mg BSA
UNOsphere S UNOsphere S Strong cation exchange -SO2- 60 mg human IgG
DEAE Macro-Prep High DEAE Weak anion exchange -HN+(C2H5)2 ≥35 mg protein
Protein Purification        
High S Macro-Prep High S Strong cation exchange -SO2- ≥49 mg human IgG
IgG Purification        
DEAE Blue DEAE Affi-Gel Blue Affinity Cibacron Blue
F3GA and DEAE
 0.2–1.0 ml serum
Antibody Purification        
Protein A Affi-Prep protein A Affinity Protein A 8–10 mg mouse monoclonal IgG,
16–23 mg human IgG
UNOSphere SUPrA Affinity media UNOsphere Affinity Recombinant Protein A 30 ± 3 mg/ml at 150 cm/hr
25 ± 2 mg/ml at 300 cm/hr
20 ± 2 mg/ml at 450 cm/hr
CHT Type I CHT ceramic
Hydroxyapitite Type I, 40 µm		 Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg Human IgG
CHT Type II CHT ceramic
Hydroxyapatite Type II, 40 µm		 Hydroxyapatite (Ca5(PO4)3OH)2 15–25 mg human IgG
Affinity-Tagged Protein Purification        
IMAC** Profinity IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
His-tagged protein
GST** Profinity GST Affinity Glutathione ≥10mg/ml recombinant
GST-tagged protein
Desalting, Buffer Exchange        
P-6 Bio-Gel P-6 Gel Filtration Polyacrylamide 10–22% CV***
Purification of Serum Proteins, Enzymes        
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
 0.2 ml serum
11 mg albumin

* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** IMAC and GST cartridges are designed for use with the Profinia protein purification system.
*** Column volume.

UNOsphere SUPrA affinity chromatography support is based on recombinant protein A and is designed for process-scale purification of monoclonal antibodies. The protein A ligand is produced in E. coli without the use of material of animal origin.

The UNOsphere base bead is a macroporous polymeric bead that is designed for robust and scalable process applications. The outstanding flow-pressure performance of UNOsphere chromatography media allows their use in large-scale process applications.

UNOsphere SUPrA affinity chromatography support comes with full regulatory support and is backed by the support of Bio-Rad’s global application and development team.

If you are interested in ordering large volume quantities, please contact your local Bio-Rad representative to discuss your requirements.

Composition Highly cross-linked polymer
Particle Size Range 53–61 µm
Ligand Recombinant Protein A
Coupling Chemistry Epoxy
Working pH range 3–11
Dynamic Binding Capacity* 30 ± 3 mg/ml at 150 cm/hr
25 ± 2 mg/ml at 300 cm/hr
20 ± 2 mg/ml 450 cm/hr
(Minimum spec: 20 mg/ml at 300 cm/hr)
Chemical Stability 10 mM hydrochloric acid
6 M guanidine hydrochloride
0.1 M arginine (pH 2.8)
0.1 M citrate (pH 2.8)
0.1 M glycine (pH 2.8)
Cleaning-In-Place (CIP) Solutions Cleaning-In-Place (CIP) Solutions 6 M guanidine hydrochloride
10 mM hydrochloric acid
0.1 M sodium hydroxide
1 M acetic acid/20% ethanol
Recommended mobile phase velocity range 100–600 cm/hr
Temperature stability 4–40°C
Delivery conditions 50% slurry in 20% ethanol
Storage conditions 4–8°C
*  10% breakthrough capacity determined with 1.0 mg/ml polyclonal human IgG in 1.1 x 10 cm column.
**No significant change in chromatographic performance 		 
UNOsphere SUPrA Affinity Chromatography Media

156-0218
25 ml, protein A affinity chromatography media

List Price:   $600.00
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UNOsphere SUPrA Affinity Chromatography Media

156-0219
100 ml, protein A affinity chromatography media

List Price:   $1,400.00
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UNOsphere SUPrA Affinity Chromatography Media

156-0220
500 ml, protein A affinity chromatography media

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UNOsphere SUPrA Affinity Chromatography Media

156-0221
5 L, protein A affinity chromatography media

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UNOsphere SUPrA Affinity Chromatography Media

156-0222
10 L, protein A affinity chromatography media

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Number Description Options
6053 Application of UNOsphere SUPrA Media, a New Protein A Affinity Chromatpgraphic Support, for Both Laboratory-Scale and Industrial Antibody Purification, Rev A Click to download [ Add to Cart ]
5729 UNOsphere SUPrA Affinity Chromatography Media Product Information Sheet, Rev B Click to download [ Add to Cart ]
5728 Monoclonal Antibody Purification Using UNOsphere SUPrA Media, Rev A Click to download [ Add to Cart ]
10014997 Instruction Manual, Bio-Scale Mini UNOsphere SUPrA cartridges, 1 and 5 ml, Rev A Click to download
10014430 Instruction Manual, UNOsphere SUPrA Affinity Chromatography Media, Rev A Click to download
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download