Bio-Scale™ Mini Profinity™ GST Cartridges

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Overview

Sizes	1 ml and 5 ml bed volumes; desalting catridges: 10 ml and 50 ml bed volumes
Recommended flow rate	Varies for each support type*
Maximum operating pressure	1, 5, and 10 ml: 45 psi 50 ml: 40 psi
Fittings	Female luer inlet and male luer outlet
Column material	Polypropylene
Frit material	Polyethylene (HDPE)
Dimensions	1 ml–40 mm length x 5.8 mm inner diameter 5 ml–40 mm length x 12.6 mm inner diameter 10 ml–80 mm length x 12.6 mm inner diameter 50 ml–80 mm length x 28.2 mm inner diameter

* Recommended flow rates are given for each support type in the instruction manual.
** Available for desalting catridges only.

Bio-Scale Mini Cartridge Selection Guide

Bio-Scale™ Mini Cartridge Selection Guide

Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*	Catalog Numbers by Cartridge Size
Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*	5 x 1 ml	1 x 5 ml	5 x 5 ml
Protein and Plasmid Purification
High Q	Macro-Prep^® High Q	Strong anion exchange	–N⁺(CH₃)₃	≥37 mg BSA	732-4120	732-4122	732-4124
UNOsphere™ Q	UNOsphereQ	Strong anion exchange	–N⁺(CH₃)₃	180 mg BSA	732-4100	732-4102	732-4104
UNOsphere S	UNOsphere S	Strong cation exchange	–SO₃^-	60 mg human lgG	732-4110	732-4112	732-4114
DEAE	Macro-Prep DEAE	Weak anion exchange	–HN⁺(C₂H₅)₂	>35 mg protein	732-4140	732-4142	732-4144
Protein Purification
High S	Macro-Prep High S	Strong cation exchange	–SO₃^-	≥49 mg human lgG	732-4130	732-4132	732-4134
IgG Purification
DEAE Blue	DEAE Affi-Gel^® Blue	Affinity	Cibacron Blue F3GA and DEAE	0.2–1.0 ml serum	—	732-4632	732-4634
Antibody Purification
Protein A	UNOsphere SUPrA™	Affinity	Recombinant protein A	30 mg/ml human IgG	732-4200, 1 x 1 ml	732-4201, 5 x 1 ml	732-4202, 1 x 5 ml
Protein A	Affi-Prep^® protein A	Affinity	Protein A	8–10 mg mouse monoclonal lgG_1; 16–23 mg human lgG	732-4600	732-4602	—
CHT Type I	CHT™ ceramic hydroxyapatite Type I, 40 µm	Hydroxyapatite	(Ca₅(PO₄)₃OH)₂	25–60 mg human lgG	—	732-4322	732-4324
CHT Type II	CHT ceramic hydroxyapatite Type II, 40 µm	Hydroxyapatite	(Ca₅(PO₄)₃OH)₂	15–25 mg human lgG	—	732-4332	732-4334
Affinity-Tagged Protein Purification
IMAC	Profinity™ IMAC	Affinity	Iminodiacetic acid	≥15 mg recombinant histidine (His)-tagged protein	732-4610	732-4612	732-4614
GST	Profinity GST	Affinity	Glutathione	≥10 mg/ml recombinant GST-tagged protein	732-4620	732-4622	732-4624
Purification of Serum Proteins, Enzymes
Blue	Affi-Gel Blue	Affinity	Cibacron Blue F3GA	0.2 ml serum 11 mg albumin	—	732-4642	732-4644

Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*	Catalog Numbers by Cartridge Size
					1 x 5 ml	5 x 5 ml	5 x 10 ml	1 x 50 ml	5 x 50 ml
Desalting, Buffer Exchange
P-6	Bio-Gel^® P-6	Gel filtration	Polyacrylamide	10–22% CV**	732- 4502	732- 4504	732- 5304	732- 5312	732- 5314

* Purification capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** Column volume.

To read more click here

Profinia Cartridge Comparison

Cartridge Comparison on Profinia System

Comparison of Bio-Scale Mini Cartridges to other affinity columns on the Profinia Protein Purification system. Yield (A) and purity (B) of various proteins purified using either Bio-Scale Mini Profinity IMAC cartridges () or commercially available prepacked columns HisTrap FF () or HisTrap HP () on the Profinia system. Rec1, 75 kD; Fih1, 37 kD; DHFR, 25 kD; SME1, 11 kD.

To read more click here

Bio-Scale Mini Profinity GST cartridges are available in 1 and 5 ml sizes. These cartridges come prepacked with Profinity GST support and are ideal for the purification of recombinant glutathione-S-transferase (GST)-tagged proteins.

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts. Bio-Scale Mini cartridges are designed for use with BioLogic systems, the Profinia protein purification system, a peristaltic pump, or any chromatography system; simple luer fittings make connections easy. For simple step elution, the cartridges can be used with a Luer-Lok syringe. The simple luer connections can connect easily to any system (see bulletin 5326).

For more information on GST, request bulletin 5513.

*U.S. patent 7,208,087.

Bio-Scale Mini Profinity GST Cartridges

732-4620
Pkg of 5, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:		$370.00
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Bio-Scale Mini Profinity GST Cartridge

732-4622
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:		$355.00
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Bio-Scale Mini Profinity GST Cartridges

732-4624
Pkg of 5, 5 ml, prepacked affinity columns, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:		$1,260.00
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Number	Description	Options
5584	Bio-Scale Mini Cartridges, Product Information Sheet, Rev B	Click to download	[ Add to Cart ] Check Out
5574	Bio-Scale Mini Profinity Cartridges Product Information Sheet, Rev A	Click to download	[ Add to Cart ] Check Out
10007406	Instruction Manual, Bio-Scale Mini Profinity GST Cartridges, 1 and 5 ml, Rev B	Click to download
5326	Chromatography Column Connection Guide, Rev A	Click to download	[ Add to Cart ] Check Out

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