Nuvia™ S Media

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Overview

Media Comparison: Dynamic Binding Capacity

Binding of polyclonal human IgG by Nuvia S media. Comparison of Nuvia S and other commercially available CEX media. Column size, 0.7 x 5.5 cm. The sample was loaded onto the column in 40 mM sodium acetate, pH 5.0 and 30 mM sodium chloride, washed, and eluted with 40 mM sodium acetate, pH 5.0 and 1 M sodium chloride.

DBC, Dynamic Binding Capacity. BT, Breakthrough

Ultra-high binding capacities provide users with the maximum value through reduced media and buffer consumption, when used in both capture and polish mode.

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Introducing Nuvia™ Q Media

Nuvia media are ultra-high binding capacity, next-generation ion exchangers designed to meet current and future process needs. Nuvia Q, our newest anion exchange media, delivers excellent resolution properties for polishing applications in downstream processes.

Get a taste of high-capacity performance with Nuvia Q and Nuvia S media.
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Tutorial: Nuvia™ Ion Exchangers

Nuvia™ Q and S Ultra-High Capacity Ion Exchange Media

Bio-Rad now offers two ultra-high capacity ion exchangers: Nuvia S, which can be used for capture and/or intermediate polishing, and Nuvia Q, which can be used for intermediate and/or final polishing.

Best-in-Class Dynamic Binding Capacity

Binding of a monoclonal antibody (mAb) by Nuvia S media. Column size, 1.1 cm x 10 cm; sample, 4.5–5.0 mg/ml mAb, was loaded onto the column in 20 mM sodium acetate, pH 5.0 + sodium chloride (adjusted for conductivity) until 5% and 10% breakthrough capacity were observed.

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Nuvia S Superior Flow Properties

Dynamic binding capacity (DBC) vs. flow velocity. A 1.1 cm diameter column was packed to a 20 cm bed height with Nuvia S media. 4.5 mg/ml human polyclonal IgG in 40 mM sodium acetate and 30 mM sodium chloride, pH 5.0 was loaded until 10% breakthrough was observed.

Nuvia S Media Chemical Stability

Stability of Nuvia S media. Results from an accelerated storage study (in 1.0 M NaOH) show no loss in dynamic binding capacity and recovery.

Base-Stability, Consistent Performance
The chemical stability of Nuvia S media allows the media to withstand repeated exposure to commonly used sanitization and clean-in-place protocols. Nuvia S media exposed to 840 hr of 1.0 M NaOH with typically used cleaning cycles showed no decreases of dynamic binding capacity or recovery. Nuvia S media delivers value and process stability by maintaining peak performance over an extended life.

Nuvia S is an ultra-high capacity, next-generation cation exchange media built on the industry proven UNOsphere™ base matrix technology. Nuvia S media provides very high capture and exceptional flow properties designed to meet current and future process needs. Nuvia S media is a flexible alternative that may be used both as a capture or polishing solution. The media delivers superior binding capacity over a wide operational window, making it the tool of choice for a wide range of downstream processes.

Nuvia S media delivers value and flexibility by allowing users to:

Use less media to purify a given amount of product
Reduce cycle time and increase productivity by operating at higher flow rates
Reduce costs and space requirements by decreasing buffer consumption
Reduce capital and operating expenses through the use of smaller columns
Chemical stability to withstand repetitive clean-in-place cycles
Flexibility in use for capture or polish steps
Fully supported for regulatory submission

Type of ion exchanger	Strong cation
Functional group	–SO₃^–
Total ionic capacity	90–150 µeq/ml
Dynamic binding capacity*: 300 cm/hr	≥110 mg/ml
Shipping counterion	Na⁺
Median particle size	85 ± 15 µm
Operational flow rate**	50–600 cm/hr
Chemical stability: 1.0 N NaOH (20ºC)	Up to 1 week
Chemical stability: 0.1 N NaOH (20ºC)***	Up to 5 years
Gel bed compression ratio	1.15–1.18 (settled bed volume/packed bed volume)
pH stability short-term***	2–14
pH stability long-term***	4–13
Shipping solution	20% ethanol + 0.1 M NaCl
Regeneration	1–2 M NaCl
Sanitation	0.5–1.0 N NaOH
Storage Conditions	20% ethanol or 0.1 N NaOH

* 10% breakthrough capacity determined with 4.5 mg/ml human IgG in 40 mM NaOAc + 30 mM NaCl, pH 5.0.
** Recommended elution flow rates are ≤ 150 cm/hr to minimize viscosity-induced backpressure.
*** Data derived under accelerated conditions at 60°C.