Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridges

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Overview

Bio-Scale Mini Cartridge Selection Guide
Bio-Scale™ Mini Cartridge Selection Guide
Cartridge by ApplicationSupportType of SeparationChemical FormProtein Capacity/ml*Catalog Numbers by Cartridge Size
5 x 1 ml1 x 5 ml5 x 5 ml
Protein and Plasmid Purification
High QMacro-Prep® High QStrong anion exchange–N+(CH3)3≥37 mg BSA732-4120732-4122732-4124
UNOsphere™ QUNOsphereQStrong anion exchange–N+(CH3)3180 mg BSA732-4100732-4102732-4104
UNOsphere SUNOsphere SStrong cation exchange–SO3-60 mg human lgG732-4110732-4112732-4114
DEAEMacro-Prep DEAEWeak anion exchange–HN+(C2H5)2>35 mg protein732-4140732-4142732-4144
Protein Purification
High SMacro-Prep High SStrong cation exchange–SO3-≥49 mg human lgG732-4130732-4132732-4134
IgG Purification
DEAE BlueDEAE Affi-Gel® Blue Affinity

Cibacron Blue

F3GA and DEAE

0.2–1.0 ml serum

—
732-4632732-4634
Antibody Purification
Protein AUNOsphere SUPrA™AffinityRecombinant protein A30 mg/ml human IgG
732-4200, 1 x 1 ml732-4201, 5 x 1 ml732-4202, 1 x 5 ml
Protein AAffi-Prep®
protein A		AffinityProtein A8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG		732-4600732-4602
—
CHT Type ICHT™ ceramic
hydroxyapatite
Type I, 40 µm		Hydroxyapatite(Ca5(PO4)3OH)225–60 mg human lgG
—
732-4322732-4324
CHT Type IICHT ceramic
hydroxyapatite
Type II, 40 µm		Hydroxyapatite(Ca5(PO4)3OH)215–25 mg human lgG
—
732-4332732-4334
Affinity-Tagged Protein Purification
IMACProfinity™ IMACAffinityIminodiacetic acid≥15 mg recombinant
histidine (His)-tagged protein		732-4610732-4612732-4614
GSTProfinity GSTAffinityGlutathione≥10 mg/ml recombinant
GST-tagged protein		732-4620732-4622732-4624
Purification of Serum Proteins, Enzymes
BlueAffi-Gel BlueAffinityCibacron Blue
F3GA		0.2 ml serum
11 mg albumin
—
732-4642732-4644

Cartridge by ApplicationSupportType of SeparationChemical FormProtein Capacity/ml*Catalog Numbers by Cartridge Size
1 x 5 ml5 x 5 ml5 x 10 ml1 x 50 ml5 x 50 ml
Desalting, Buffer Exchange
P-6Bio-Gel® P-6Gel filtrationPolyacrylamide10–22% CV**732-
4502		732-
4504		732-
5304		732-
5312		732-
5314

* Purification capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** Column volume.

PDF Bulletin 5584 To read more click here

Cartridge Comparison
Cartridge Comparison on Profinia System

Comparison of Bio-Scale mini cartridges to other affinity columns on the Profinia™ protein purification system. Yield (A) and purity (B) of various proteins purified using either Bio-Scale™ Mini Profinity™ IMAC cartridges () or commercially available prepacked columns HisTrap FF () or HisTrap HP () on the Profinia system. Rec1, 75 kD; Fih1, 37 kD; DHFR, 25 kD; SME1, 11 kD.

To read more click here

Bio-Gel P Properties
Bio-Gel P Properties
  Hydrated Bead Size (µm) Hydrated Bed Volume Mass Dry Gel Typical Flow Rate (cm/hr)* Molecular Weight Fractionation Range
Bio-Gel P–2, fine 45–90 3 ml/g 5–10 100–1,800
Bio-Gel P–2, extra fine <45   <10  
Bio-Gel P–4, medium 90–180 4 ml/g 15–20 800–4,000
Bio-Gel P–4, fine 45–90   10–15  
Bio-Gel P–4, extra fine <45   <10  
Bio-Gel P–6, medium 90–180 6.5 ml/g 15–20 1,000–6,000
Bio-Gel P–6, fine 45–90   10–15  
Bio-Gel P–6, extra fine <45   <10  
Bio-Gel P–6DG gel 90–180   15–20  
Bio-Gel P–10 gel, medium 90–180 7.5 ml/g 15–20 1,500–20,000
Bio-Gel P–10 gel, fine 45–90   10–15  
Bio-Gel P–30 gel, medium 90–180 9 ml/g 7–13 2,400–40,000
Bio-Gel P–30 gel, fine 45–90   6–11  
Bio-Gel P–60 gel, medium 90–180 11 ml/g 4–6 3,000–60,000
Bio-Gel P–60 gel, fine 45–90   3–5  
Bio-Gel P–100 gel, medium 90–180 12 ml/g 4–6 5,000–100,000
Bio-Gel P–100 gel, fine 45–90   3–5  

* Row rates determined in a 1.5 x 70 cm column using a hydrostatic pressure head-to-bed ratio of 1:1.

Bio-Scale Mini Bio-Gel P-6 cartridges are filled with Bio-Gel P-6 gel and are convenient, disposable, and ready to use. The cartridges come in 5, 10, and 50 ml formats that can be used for desalting or buffer exchange applications using gel filtration (size exclusion) chromatography.

Bio-Scale Mini cartridges have a polypropylene double-wall construction* that provides extra strength and allows reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Bio-Scale Mini cartridges are ideal for low-pressure chromatography applications. Luer fittings make connections simple; adaptors are available to allow connection to any chromatography system (see bulletin 5326, Column Connection Guide).

*U.S. patent 7,208,087.

Sizes 1 ml and 5 ml bed volumes; desalting cartridges: 10ml and 50 ml bed volumes
Recommended flow rate Varies for each support type*
Maximum operating pressure 1, 5, and 10 ml: 45 psi 50ml: 40 psi
Fittings Female luer inlet and male luer outlet
Column material Polypropylene
Frit material Polyethylene (HDPE)
Dimensions 1 ml – 40 mm length x 5.6 mm inner diameter 5 ml – 40 mm length x 12.6 mm inner diameter 10 ml – 80 mm length x 12.6 mm inner diameter** 50 ml – 80 mm length x 28.2 mm inner diameter**

*Recommended flow rates are given for each support type in the instruction manual.
**Available for desalting cartridges only.

  
Bio-Scale Mini Bio-Gel P-6 Desalting Cartridges

732-5304
Pkg of 5, 10 ml, prepacked gel filtration column, 80 mm x 12.6 mm, max. pressure 45 psi, for use with Profinia protein purification system

List Price:   $340.00
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Bio-Scale Mini Bio-Gel P-6 Desalting Cartridge

732-5312
Pkg of 1, 50 ml, prepacked gel filtration column, 80 mm x 28.2 mm, max. pressure 40 psi, for use with Profinia protein purification system

List Price:   $335.00
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Bio-Scale Mini Bio-Gel P-6 Desalting Cartridges

732-5314
Pkg of 5, 50 ml, prepacked gel filtration column, 80 mm x 28.2 mm, max. pressure 40 psi, for use with Profinia protein purification system

List Price:   $1,365.00
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Number Description Options
10006995 Bio-Scale Mini Bio-Gel P-6 Desalting Cartridges, 10 and 50 ml, Instruction Manual, Rev B Click to download
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download
5539 Automated Desalting of Proteins With the Profinia Protein Purification System: Comparison to Manual Desalting by Dialysis, Rev A Click to download [ Add to Cart ]
2068 Desalting With Bio-Gel P-6DG Desalting Gel, Rev B Click to download
5574 Bio-Scale Mini Profinity Cartridges Product Information Sheet, Rev A Click to download [ Add to Cart ]
5342 Automated Three-Dimensional Purification Method for Histidine-Tagged Proteins Using the BioLogic DuoFlow Maximizer Chromatography System, Rev A Click to download
LIT174 Instruction Manual, Bio-Gel P Polyacrylamide Gel, Rev B Click to download [ Add to Cart ]
LIT507 Instruction Manual, Bio-Spin 6 and 30 Columns, Rev G Click to download [ Add to Cart ]
5326 Chromatography Column Connection Guide, Rev A Click to download [ Add to Cart ]