Bio-Scale Mini Profinity eXact cartridges are 1 and 5 ml cartridges filled with Profinity eXact purification resin. Profinity eXact purification resin consists of a highly engineered subtilisin protease conjugated to an agarose-based matrix. The cartridges are used for on-column purification and cleavage of recombinant proteins using the Profinity eXact fusion-tag system. The novel Profinity eXact fusion-tag system generates a highly purified recombinant protein containing only its native amino acid sequence in a single step and in a fraction of the time of other methods.
Profinity eXact benefits:
- Purification and processing of fusion-tagged proteins in a single step
- On-column cleavage in as little as 30 minutes
- No protease addition required
- Precise cleavage at N-terminus to generate native protein sequence
Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts. Bio-Scale Mini cartridges are designed for use with BioLogic systems, the Profina protein purification system, a peristaltic pump, or any chromatography system. The simple luer connections can connect easily to any system (see bulletin 5326).
For more information, request bulletin 5655.
*U.S. patent 7,208,087.