Bio-Scale™ Mini UNOsphere SUPrA™ Affinity Cartridges

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Overview

Composition	Highly cross-linked polymer
Particle Size Range	53–61 µm
Ligand	Recombinant Protein A
Coupling Chemistry	Epoxy
Working pH range	3–11
Dynamic Binding Capacity*	30 ± 3 mg/ml at 150 cm/hr 25 ± 2 mg/ml at 300 cm/hr 20 ± 2 mg/ml 450 cm/hr (Minimum spec: 20 mg/ml at 300 cm/hr)
Chemical Stability	10 mM hydrochloric acid 6 M guanidine hydrochloride 0.1 M arginine (pH 2.8) 0.1 M citrate (pH 2.8) 0.1 M glycine (pH 2.8)
Cleaning-In-Place (CIP) Solutions	6 M guanidine hydrochloride 10 mM hydrochloric acid 0.1 M sodium hydroxide 1 M acetic acid/20% ethanol
Recommended mobile phase velocity range	100–600 cm/hr
Temperature stability	4–40°C
Delivery conditions	50% slurry in 20% ethanol
Storage conditions	4–8°C

* 10% breakthrough capacity determined with 1.0 mg/ml polyclonal human IgG in 1.1 x 10 cm column.
** No significant change in chromatographic performance

mAb Workflow

Monoclonal Antibody Workflow

Workflow for mAb purification with UNOsphere SUPrA affinity, UNOsphere Q, and CHT Type 1 media.

To read more click here

Cartridge Selection by Application

Prepacked Cartridge Selection by Application


Cartridge by Application	Support	Type of Separation	Chemical Form	Binding Capacity

Protein and Plasmid Purification
UNOSphere Rapid S media	UNOSphere Rapid S	Strong cation exchange	SO₃-	* Human IgG: 60 mg/ml
CFT media	Ceramic fluoropatite	Fluoropatite	Ca₁₀(PO₄)₆F₂	Lysozyme 15–21 mg/g
Apatite purification kit	Ceramic hydroxyapatite Ceramic fluoropatite	Hydroxyapatite Fluoropatite	(Ca₅(PO₄)₃OH)₂ Ca₁₀(PO₄)₆F₂	** Human IgG; 25-60 mg/ml Lysozyme 15–21 mg/g
Protein Purification
UNOSphere Rapid S media	UNOSphere Rapid S	Strong cation exchange	SO₃-	* Human IgG: 60 mg/ml
CFT media	Ceramic fluoropatite	Fluoropatite	Ca₁₀(PO₄)₆F₂	Lysozyme 15–21 mg/g
Apatite purification kit	Ceramic hydroxyapatite Ceramic fluoropatite	Hydroxyapatite Fluoropatite	(Ca₅(PO₄)₃OH)₂ Ca₁₀(PO₄)₆F₂	** Human IgG; 25–60 mg/ml Lysozyme 15–21 mg/g
Antibody Purification
CFT Media	Ceramic fluoropatite	Fluoropatite	Ca₁₀(PO₄)₆F₂	Lysozyme 15–21 mg/g
UNOsphere SUPrA media	UNOsphere SUPrA	Affinity	Protein A
Monoclonal Antibody Purification
mAb purification kit	UNOsphere SUPrA CHT Type 1 UNOsphere Q	Affinity Hydroxyapatite Strong anion exchange	Protein A (Ca₅(PO₄)₃OH)₂ -N(CH₃)₃*	** Human IgG: 25–60 mg/ml * BSA 180mg/ml

* Flow rate = 150 cm/hr; ** flow rate = 300 cm/hr

Mini Cartridge Specifications

Bio-Scale Mini Cartridge Specifications


Sizes	1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate	Varies for each support type*
Maximum operating pressure	1, 5, and 10 ml: 45 psi 50 ml: 40 psi
Fittings	Female luer inlet and male luer outlet
Column material	Polypropylene
Frit material	Polyethylene (HDPE)
Dimensions	1 ml — 40 mm length x 5.6 mm inner diameter 5 ml — 40 mm length x 12.6 mm inner diameter 10 ml — 80 mm length x 12.6 mm inner diameter 50 ml — 80 mm length x 28.2 mm inner diameter

* Recommended flow rates are given for each support type in the instruction manual.
** Available for desalting cartridges only.

Bio-Scale Mini Cartridge Selection Guide

Bio-Scale™ Mini Cartridge Selection Guide

Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*	Catalog Numbers by Cartridge Size
Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*	5 x 1 ml	1 x 5 ml	5 x 5 ml
Protein and Plasmid Purification
High Q	Macro-Prep^® High Q	Strong anion exchange	–N⁺(CH₃)₃	≥37 mg BSA	732-4120	732-4122	732-4124
UNOsphere™ Q	UNOsphereQ	Strong anion exchange	–N⁺(CH₃)₃	180 mg BSA	732-4100	732-4102	732-4104
UNOsphere S	UNOsphere S	Strong cation exchange	–SO₃^-	60 mg human lgG	732-4110	732-4112	732-4114
DEAE	Macro-Prep DEAE	Weak anion exchange	–HN⁺(C₂H₅)₂	>35 mg protein	732-4140	732-4142	732-4144
Protein Purification
High S	Macro-Prep High S	Strong cation exchange	–SO₃^-	≥49 mg human lgG	732-4130	732-4132	732-4134
IgG Purification
DEAE Blue	DEAE Affi-Gel^® Blue	Affinity	Cibacron Blue F3GA and DEAE	0.2–1.0 ml serum	—	732-4632	732-4634
Antibody Purification
Protein A	UNOsphere SUPrA™	Affinity	Recombinant protein A	30 mg/ml human IgG	732-4200, 1 x 1 ml	732-4201, 5 x 1 ml	732-4202, 1 x 5 ml
Protein A	Affi-Prep^® protein A	Affinity	Protein A	8–10 mg mouse monoclonal lgG_1; 16–23 mg human lgG	732-4600	732-4602	—
CHT Type I	CHT™ ceramic hydroxyapatite Type I, 40 µm	Hydroxyapatite	(Ca₅(PO₄)₃OH)₂	25–60 mg human lgG	—	732-4322	732-4324
CHT Type II	CHT ceramic hydroxyapatite Type II, 40 µm	Hydroxyapatite	(Ca₅(PO₄)₃OH)₂	15–25 mg human lgG	—	732-4332	732-4334
Affinity-Tagged Protein Purification
IMAC	Profinity™ IMAC	Affinity	Iminodiacetic acid	≥15 mg recombinant histidine (His)-tagged protein	732-4610	732-4612	732-4614
GST	Profinity GST	Affinity	Glutathione	≥10 mg/ml recombinant GST-tagged protein	732-4620	732-4622	732-4624
Purification of Serum Proteins, Enzymes
Blue	Affi-Gel Blue	Affinity	Cibacron Blue F3GA	0.2 ml serum 11 mg albumin	—	732-4642	732-4644

Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*	Catalog Numbers by Cartridge Size
					1 x 5 ml	5 x 5 ml	5 x 10 ml	1 x 50 ml	5 x 50 ml
Desalting, Buffer Exchange
P-6	Bio-Gel^® P-6	Gel filtration	Polyacrylamide	10–22% CV**	732- 4502	732- 4504	732- 5304	732- 5312	732- 5314

* Purification capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** Column volume.

To read more click here

Flow Performance

Flow Performance of UNOsphere SUPrA Media

Narrow elution profile of antibody on UNOsphere SUPrA media.

UNOsphere SUPrA affinity chromatography support is based on recombinant protein A and is designed for process-scale purification of monoclonal antibodies. The protein A ligand is produced in E. coli without the use of material of animal origin.

Bio-Scale Mini cartridges have a polypropylene double-wall construction* that provides extra strength and allows reliable runs up to 45 psi with the aqueous buffers most commonly used for protein separations. Bio-Scale Mini cartridges are ideal for low-pressure chromatography applications. Luer fittings make connections simple; adapters are available to allow connection to all types of chromatography systems.

Bio-Scale Mini UNOsphere SUPrA cartridges are available in 1 ml and 5 ml sizes. These cartridges are prepacked with UNOsphere SUPrA affinity media and are ideal for method scouting and small-scale purifications.

* U.S. Patent 7,208,087.

Bio-Scale™ Mini UNOsphere™ Rapid S cartridges, 5 x 1ml

732-4400
Pkg of 5, 1 ml, prepacked, UNOsphere rapid S cation exchange media cartridge

List Price:		$185.00
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Bio-Scale™ Mini UNOsphere™ Rapid S cartridges, 1 x 5 ml

732-4401
5 ml, prepacked, UNOsphere rapid S cation exchange media cartridge

List Price:		$130.00
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Bio-Scale™ Mini UNOsphere™ Rapid S cartridges, 5 x 5 ml

732-4402
Pkg of 5, 5 ml, prepacked, UNOsphere rapid S cation exchange media cartridge

List Price:		$450.00
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Bio-Scale Mini UNOsphere SUPrA affinity chromatography cartridges, 1 x 1 ml

732-4200
1 ml, prepacked, UNOsphere SUPrA affinity media cartridge

List Price:		$140.00
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Bio-Scale Mini UNOsphere SUPrA affinity chromatography cartridges, 5 x 1 ml

732-4201
Pkg of 5, 1 ml, prepacked, UNOsphere SUPrA affinity media cartridge

List Price:		$605.00
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Bio-Scale Mini UNOsphere SUPrA affinity chromatography cartridges, 1 x 5 ml

732-4202
5 ml, prepacked, UNOsphere SUPrA affinity media cartridge

List Price:		$515.00
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Number	Description	Options
5729	UNOsphere SUPrA Affinity Chromatography Media Product Information Sheet, Rev B	Click to download	[ Add to Cart ] Check Out
5728	Monoclonal Antibody Purification Using UNOsphere SUPrA Media, Rev A	Click to download	[ Add to Cart ] Check Out
10014997	Instruction Manual, Bio-Scale Mini UNOsphere SUPrA cartridges, 1 and 5 ml, Rev A	Click to download
10014430	Instruction Manual, UNOsphere SUPrA Affinity Chromatography Media, Rev A	Click to download
5326	Chromatography Column Connection Guide, Rev A	Click to download	[ Add to Cart ] Check Out
5662	Process Chromatography Applications Laboratory Brochure, Rev B	Click to download	[ Add to Cart ] Check Out
5584	Bio-Scale Mini Cartridges, Product Information Sheet, Rev B	Click to download	[ Add to Cart ] Check Out

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