Macro-Prep 25 S Media

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Overview

Pressure versus Linear Velocity

Pressure vs. linear velocity for 1 M NaCl on Macro-Prep 25 S in a 1.6 cm (ID) x 12.5 cm (L) column.

Macro-Prep 25 S strong cation exchange support offers high-resolution separations at high flow rates with medium pressures. It possesses the same rigid, macroporous, and hydrophilic properties of 50 µm Macro-Prep High S support but in a 25 µm bead that offers higher resolution.

Benefits include:

Benchtop to pilot-scale separations, where maximum sample recovery and purity are important
Fewer chromatographic steps required
Removal of protein variants and other difficult contaminants that would require additional validation if present in the final formulation

Type of ion exchanger	Strong cation
Functional group	–SO^3-
Total ionic capacity	110±30 µeq/ml
Median particle size	25 µm
Nominal pore size	725 Å
Shipping counterion	NA⁺
Dynamic binding capacity	>40 mg/ml lgG
Recommended linear flow rate range	50–300 cm/hr
Maximum linear flow rate	3,000 cm/hr
Chemical stability
1.0 M HCI	>72 hr
100% ethanol	Excellent
Chaotropic agents (8 M urea, 6 M guanidine-HCI)	Excellent
pH stability	1–12
Volume changes
ph 4–10	<1%
0.1–1.0 M Na Cl	<4%
Autoclavability (121°C, 30 min)	Yes
Storage solution	20% ethanol