Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. The cartridges are prepacked with Bio-Rad's chromatography media for ion exchange, affinity, hydroxyapatite, and fluoroapatite media. Convenient, disposable, and ready to use, these cartridges come in 1 and 5 ml formats that can be used for method scouting or simple purifications (larger 10 and 50 ml desalting cartridges are also available). The simple luer connections can connect easily to any chromatography system (for a column connection guide, refer to bulletin 5326).
The Bio-Scale Mini cartridge protein purification sampler packs offer a quick way to experiment with separation techniques when the protein to be purified has not yet been characterized. With the following sampler packs, a sample can be tested with all the cartridges in a single day.
Bio-Scale Mini Apatite Purification Kit
CHT ceramic hydroxyapatite and CFT ceramic fluoroapatite have comparable separation characteristics, differing mostly in the pH buffer range in which they perform best. Whereas CHT ceramic hydroxyapatite exhibits pH stability as low as pH 6.5, the fluorine substitution in CFT ceramic fluorapatite extends pH stability to as low as pH 5.6. The Bio-Scale Mini apatite purification kit is a convenient way to evaluate which apatite material provides the optimum chromatographic performance for your application.
Features include:
- Prepacked columns with CHT ceramic hydroxyapatite and CFT ceramic fluoroapatite for convenient process development
- Low bed volume allowing minimum requirements for sample and buffer
- Luer fittings for convenient connection to any chromatographic system
- Distinct selectivities and different pH ranges
Bio-Scale Mini mAb Purification Kit
Maximize your method optimization and parameter screening for monoclonal antibodies with this sampler kit. This convenient process development kit contains cartridges filled with UNOsphere SUPrA affinity, UNOsphere Q, and CHT ceramic hydroxyapatite media to address all your needs for monoclonal capture, intermediate contaminant removal, and polishing. Maximize your downstream process by optimizing your process application using this kit.
Benefits include:
- Convenient prepacked columns with a simple luer fitting for easy connection to any system
- Ideal for screening and optimization of purification protocols
- Reproducible and scalable results
Bio-Scale Mini Ion Exchange Sampler Pack
This sampler pack is used to assess and compare the separation characteristics of a variety of Bio-Rad's most popular ion exchange media. It includes an UNOsphere Q cartridge, an UNOsphere S cartridge, a Macro-Prep Q cartridge, a Macro-Prep S cartridge, and a Macro-Prep DEAE cartridge, all in 1 ml formats.
Bio-Scale Mini Affinity Sampler Pack
This sampler pack is used to develop or compare purification schemes using a variety of affinity purification media. The Bio-Scale Mini affinity sampler pack includes a 1 ml Profinity IMAC cartridge, 1 ml Profinity GST cartridge, 1 ml Affi-Prep protein A cartridge, 5 ml DEAE Affi-Gel Blue cartridge, and 5 ml Affi-Gel Blue cartridge.
* U.S. patent 7,308,087 B2.
