UNOsphere™ Rapid S Media

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Overview

Stability of UNOsphere Rapid S

Stability of UNOsphere Rapid S media. Results from an accelerated storage study (in 0.1 M NaOH) show no loss in dynamic binding capacity in an equivalent-to-5-year test. DBC, dynamic binding capacity.

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Media Performance

Pressure/Flow Performance of UNOsphere Rapid S

UNOsphere Rapid S media performance. Pressure/flow performance for a 20 cm diameter x 20 cm bed height column packed to 17% axial compression.

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Dynamic Binding Capacity

UNOsphere Rapid S Media Dynamic Binding Capacity

Dynamic binding capacity (DBC) vs. flow velocity. A column 1.1 cm in diameter was packed to a 20 cm bed height with UNOsphere Rapid S media; 2 mg/ml human IgG in 50 mM sodium acetate was loaded until 10% breakthrough was observed.

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IgG Purification

An example of separation. A, chromatogram of IgG purification from calf serum (green trace) and purified bovine IgG (blue trace) using the the same binding and elution conditions. B, chromatography conditions.

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UNOsphere Rapid S strong cation exchange media have been optimized to deliver speed and throughput in capture and intermediate purification steps. The support combines high binding capacity, fast mass transfer, excellent flow characteristics, and robust physical properties to make it an exceptional choice for process chromatography.

UNOsphere Rapid S support:

Stands up to sanitization and cleaning — The performance of UNOsphere Rapid S support is not compromised by exposure to NaOH in commonly used sanitization and clean-in-place protocols
Exhibits high flow at low backpressure — The macroporous structure of the UNOsphere matrix affords the use of high flow rates while maintaining very low backpressures. With typical buffers and running conditions, it is possible to achieve linear velocities in excess of 1,000 cm/hr with backpressures below 2 bar
Is designed for reproducibility — UNOsphere media are based on a unique polymerization process, which yields macroporous, hydrophilic beads that are ideal for biomolecule separations. Incorporation of the sulfonic acid ligand directly into the matrix during the polymerization process ensures consistent batch-to-batch and run-to-run reproducibility

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO 9001-registered corporation.

For more information, request bulletin 5734.

Type of ion exchanger	Strong cation
Functional group	-SO₃^-
Total ionic capacity	280 ± 30 µeq/ml
Dynamic binding capacity*
150 cm/hr	60 mg IgG/ml
1,200 cm/hr	30 mg IgG/ml
Median particle size	80 µm
Recommended linear flow rate range**	50–1,200 cm/hr
Chemical stability
1.0 M NaOH (20°C)	Up to 2,000 hr
1.0 M HCl (20°C)	Up to 200 hr
pH stability range	pH 1–14
Regeneration conditions	1–2 M NaCl
Storage conditions	20% Ethanol 0.1 N NaOH for 30 days
* 10% breakthrough capacity determined with 4.5 mg/ml human IgG in a 1.1 x 10 cm column.
** UNOsphere S support packed into a 20 cm bed height and run at 1,200 cm/hr generates less than 2 bar backpressure.