Bio-Scale™ Mini CFT™ Ceramic Fluoroapatite Cartridges

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Overview

Bio-Scale Mini Cartridge Specifications


Sizes	1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate	Varies for each support type*
Maximum operating pressure	1, 5, and 10 ml: 45 psi 50 ml: 40 psi
Fittings	Female luer inlet and male luer outlet
Column material	Polypropylene
Frit material	Polyethylene (HDPE)
Dimensions	1 ml — 40 mm length x 5.6 mm inner diameter 5 ml — 40 mm length x 12.6 mm inner diameter 10 ml — 80 mm length x 12.6 mm inner diameter 50 ml — 80 mm length x 28.2 mm inner diameter

* Recommended flow rates are given for each support type in the instruction manual.
** Available for desalting cartridges only.

Bio-Scale Mini Cartridge Selection Guide

Bio-Scale™ Mini Cartridge Selection Guide

Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*	Catalog Numbers by Cartridge Size
Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*	5 x 1 ml	1 x 5 ml	5 x 5 ml
Protein and Plasmid Purification
High Q	Macro-Prep^® High Q	Strong anion exchange	–N⁺(CH₃)₃	≥37 mg BSA	732-4120	732-4122	732-4124
UNOsphere™ Q	UNOsphereQ	Strong anion exchange	–N⁺(CH₃)₃	180 mg BSA	732-4100	732-4102	732-4104
UNOsphere S	UNOsphere S	Strong cation exchange	–SO₃^-	60 mg human lgG	732-4110	732-4112	732-4114
DEAE	Macro-Prep DEAE	Weak anion exchange	–HN⁺(C₂H₅)₂	>35 mg protein	732-4140	732-4142	732-4144
Protein Purification
High S	Macro-Prep High S	Strong cation exchange	–SO₃^-	≥49 mg human lgG	732-4130	732-4132	732-4134
IgG Purification
DEAE Blue	DEAE Affi-Gel^® Blue	Affinity	Cibacron Blue F3GA and DEAE	0.2–1.0 ml serum	—	732-4632	732-4634
Antibody Purification
Protein A	UNOsphere SUPrA™	Affinity	Recombinant protein A	30 mg/ml human IgG	732-4200, 1 x 1 ml	732-4201, 5 x 1 ml	732-4202, 1 x 5 ml
Protein A	Affi-Prep^® protein A	Affinity	Protein A	8–10 mg mouse monoclonal lgG_1; 16–23 mg human lgG	732-4600	732-4602	—
CHT Type I	CHT™ ceramic hydroxyapatite Type I, 40 µm	Hydroxyapatite	(Ca₅(PO₄)₃OH)₂	25–60 mg human lgG	—	732-4322	732-4324
CHT Type II	CHT ceramic hydroxyapatite Type II, 40 µm	Hydroxyapatite	(Ca₅(PO₄)₃OH)₂	15–25 mg human lgG	—	732-4332	732-4334
Affinity-Tagged Protein Purification
IMAC	Profinity™ IMAC	Affinity	Iminodiacetic acid	≥15 mg recombinant histidine (His)-tagged protein	732-4610	732-4612	732-4614
GST	Profinity GST	Affinity	Glutathione	≥10 mg/ml recombinant GST-tagged protein	732-4620	732-4622	732-4624
Purification of Serum Proteins, Enzymes
Blue	Affi-Gel Blue	Affinity	Cibacron Blue F3GA	0.2 ml serum 11 mg albumin	—	732-4642	732-4644

Cartridge by Application	Support	Type of Separation	Chemical Form	Protein Capacity/ml*	Catalog Numbers by Cartridge Size
					1 x 5 ml	5 x 5 ml	5 x 10 ml	1 x 50 ml	5 x 50 ml
Desalting, Buffer Exchange
P-6	Bio-Gel^® P-6	Gel filtration	Polyacrylamide	10–22% CV**	732- 4502	732- 4504	732- 5304	732- 5312	732- 5314

* Purification capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** Column volume.

To read more click here

mAb Workflow

Monoclonal Antibody Workflow

Workflow for mAb purification with UNOsphere SUPrA affinity, UNOsphere Q, and CHT Type 1 media.

To read more click here

CFT Specifications

	Type I	Type II
Functional groups	Ca²⁺, PO₄^3-, F-	Ca²⁺, PO₄^3-, F-
Observed dynamic binding capacity bovine lgG	14–17 mg/g CFT	30–32 mg/g CFT
Observed dynamic binding capacity lysozyme (Lys)	≥25 mg/g CFT	17–21 mg/g CFT
Nominal pore diameter	600–800 A	600–800 A
Maximum backpressure	55 bar (800 psi)	55 bar (800 psi)
Nominal mean particle size	40 ± 4 µm	40 ± 4 µm

CFT Characteristics

	Types I and II
Typical linear flow rate range	50–300 cm/hr
pH Stability	5–14 pH
Base Stability
Regeneration	Normal — 400 mM sodium phosphate, pH6.8 Difficult — 400 mM trisodium phosphate, pH 11–12 Basic proteins (pl >7)– 1–2 M NaCl or KCI,pH 10–12
Sanitation	Perform CIP, then 3–5 CV 1–2 N NaOH or KOH; exposure time ≥1 hr
Recommended column storage	0.1–1 N NaOH or KOH
Shelf life (dry, unused material)	>2 years

* 40 µm particles, 300 cm/hr, 5 mM sodium phosphate, pH 6.5.
** 40 µm particles, 300 cm/hr, 5 mM sodium phosphate, pH 6.5.

Bio-Scale Mini CFT ceramic fluoroapatite cartridges are 5 ml cartridges filled with CFT ceramic fluoroapatite support. CFT ceramic fluoroapatite (Ca₁₀(PO₄)₆F₂) is a rigid, spherical macroporous support used in the purification of biologically active compounds. CFT is a composite of fluoroapatite and hydroxyapatite prepared by chemically converting hydroxyapatite nanocrystals to fluoroapatite with a fluorine reagent. CFT can be used under stringent chromatography conditions to separate acidic proteins requiring buffered conditions as low as pH 5.6. The cartridges can be used for method scouting or simple purifications.

CFT has high binding capacity and may be used reproducibly over an extended number of chromatography runs. Its increased tensile strength, chemical durability, and density provide excellent throughput and consistent performance for all separations, including biopharmaceutical process-scale manufacturing.

Features of CFT include:

High binding capacity
Sintering at 700°C (Type II) for heavy-duty, durable supports
Rigid particles for fast cleaning and equilibration
Inorganic calcium phosphate for distinct selectivities

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts. Bio-Scale Mini cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system; simple luer fittings make connection to any system easy (see bulletin 5326).

*U.S. patent 7,208,087.

Bio-Scale Mini CFT Type II cartridges, 1 x 5 ml

732-4405
5 ml, prepacked, CFT ceramic fluoroapatite multimodal chromatography media cartridge, Type II

List Price:		$140.00
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Bio-Scale Mini CFT Type II cartridges, 5 x 5 ml

732-4406
Pkg of 5, 5 ml, prepacked, CFT ceramic fluoroapatite multimodal chromatography media cartridge, Type II

List Price:		$560.00
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Number	Description	Options
5584	Bio-Scale Mini Cartridges, Product Information Sheet, Rev B	Click to download	[ Add to Cart ] Check Out
5326	Chromatography Column Connection Guide, Rev A	Click to download	[ Add to Cart ] Check Out

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