Bio-Scale™ Mini UNOsphere™ Q Cartridges

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Overview

Bio-Scale Mini Cartridge Specifications
Sizes 1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate Varies for each support type*
Maximum operating pressure 1, 5, and 10 ml: 45 psi
50 ml: 40 psi
Fittings Female luer inlet and male luer outlet
Column material Polypropylene
Frit material Polyethylene (HDPE)
Dimensions 1 ml — 40 mm length x 5.6 mm inner diameter
5 ml — 40 mm length x 12.6 mm inner diameter
10 ml — 80 mm length x 12.6 mm inner diameter**
50 ml — 80 mm length x 28.2 mm inner diameter**

* Recommended flow rates are given for each support type in the instruction manual.
** Available for desalting cartridges only.

Monoclonal Antibody Workflow

Workflow for mAb purification with UNOsphere SUPrA affinity, UNOsphere Q, and CHT Type 1 media.

To read more click here

Bio-Scale™ Mini Cartridge Selection Guide
Cartridge by ApplicationSupportType of SeparationChemical FormProtein Capacity/ml*Catalog Numbers by Cartridge Size
5 x 1 ml1 x 5 ml5 x 5 ml
Protein and Plasmid Purification
High QMacro-Prep® High QStrong anion exchange–N+(CH3)3≥37 mg BSA732-4120732-4122732-4124
UNOsphere™ QUNOsphereQStrong anion exchange–N+(CH3)3180 mg BSA732-4100732-4102732-4104
UNOsphere SUNOsphere SStrong cation exchange–SO3-60 mg human lgG732-4110732-4112732-4114
DEAEMacro-Prep DEAEWeak anion exchange–HN+(C2H5)2>35 mg protein732-4140732-4142732-4144
Protein Purification
High SMacro-Prep High SStrong cation exchange–SO3-≥49 mg human lgG732-4130732-4132732-4134
IgG Purification
DEAE BlueDEAE Affi-Gel® Blue Affinity

Cibacron Blue

F3GA and DEAE

0.2–1.0 ml serum

732-4632732-4634
Antibody Purification
Protein AUNOsphere SUPrA™AffinityRecombinant protein A30 mg/ml human IgG
732-4200, 1 x 1 ml732-4201, 5 x 1 ml732-4202, 1 x 5 ml
Protein AAffi-Prep®
protein A
AffinityProtein A8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG
732-4600732-4602
CHT Type ICHT™ ceramic
hydroxyapatite
Type I, 40 µm
Hydroxyapatite(Ca5(PO4)3OH)225–60 mg human lgG
732-4322732-4324
CHT Type IICHT ceramic
hydroxyapatite
Type II, 40 µm
Hydroxyapatite(Ca5(PO4)3OH)215–25 mg human lgG
732-4332732-4334
Affinity-Tagged Protein Purification
IMACProfinity™ IMACAffinityIminodiacetic acid≥15 mg recombinant
histidine (His)-tagged protein
732-4610732-4612732-4614
GSTProfinity GSTAffinityGlutathione≥10 mg/ml recombinant
GST-tagged protein
732-4620732-4622732-4624
Purification of Serum Proteins, Enzymes
BlueAffi-Gel BlueAffinityCibacron Blue
F3GA
0.2 ml serum
11 mg albumin
732-4642732-4644

Cartridge by ApplicationSupportType of SeparationChemical FormProtein Capacity/ml*Catalog Numbers by Cartridge Size
1 x 5 ml5 x 5 ml5 x 10 ml1 x 50 ml5 x 50 ml
Desalting, Buffer Exchange
P-6Bio-Gel® P-6Gel filtrationPolyacrylamide10–22% CV**732-
4502
732-
4504
732-
5304
732-
5312
732-
5314

* Purification capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** Column volume.

PDF Bulletin 5584 To read more click here

Purification of Plasmid DNA

Purification of plasmid DNA on a UNOsphere Q column. Clarified bacterial lysate (10 ml, adjusted to ph 8.0) was loaded onto a 0.5 x 11 cm column (2.1 ml) in buffer A (10 mm sodium phosphate, 0.3 m NaCl, 1 mm EDTA, ph 8.0). the sample was eluted with a 0–40% gradient of buffer B (10 mm sodium phosphate, 1.0 m NaCl, 1 mm EDTA, ph 8.0) at a flow rate of 2 ml/min (600 cm/hr). the column was washed with 10 column volumes of 40% buffer B, followed by 100% buffer B for 5 column volumes. the effluent was monitored at 254 nm. Each fraction was 5 ml. Blue, A254; red, conductivity; black, theoretical gradient.


To read more click here

Analysis of Plasmid DNA purified on UNOsphere Q support

Analysis of plasmid DNA purified on UNOsphere Q support. Fractions from the chromatography run were separated on a 0.8% agarose gel. Lane 1, 1 kb marker (catalog #170-8204); lane 2, crude lysate; lane 3, flowthrough (fractions 2–6); lane 4, fractions 9–18; lane 5, fractions 19–30; lane 6, fractions 31–35; lane 7, fraction 36.


To read more click here

Bio-Scale Mini UNOsphere Q cartridges are 1 ml and 5 ml cartridges filled with UNOsphere Q strong anion exchange media. These cartridges can be used for method scouting and small-scale purifications.

UNOsphere Q support meets the needs of the biopharmaceutical industry for high-productivity chromatographic media. The UNOsphere hydrophilic polymeric support delivers high productivity through high binding capacity and low backpressures. The single-step polymerization produces a support unsurpassed in quality and batch-to-batch reproducibility.

Features of UNOsphere Q ion exchange support include:

  • Optimized to operate under 2 bar backpressure at linear velocities of 1,200 cm/hr
  • Stable to 1 M NaOH exposure up to 10,000 hours
  • Efficient capture of biopharmaceutical molecules from crude feedstreams
  • Large pores that result in ultrahigh binding capacities at fast linear velocities
  • Hydrophilic polymeric beads
  • Robust polymer designed to withstand repeated clean-in-place cycles
  • Biopharmaceutical manufacturing quantities available
  • Fully supported for regulatory submission

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, disposable, and ready to use, these cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system. Simple luer fittings make connection to any system easy.

* U.S. Patent 7,208,087.

Bio-Scale Mini UNOsphere Q Cartridges

732-4100
Pkg of 5, 1 ml, prepacked ion exchange column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $190.00
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Bio-Scale Mini UNOsphere Q Cartridge

732-4102
Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $135.00
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Bio-Scale Mini UNOsphere Q Cartridges

732-4104
Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $465.00
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Number Description Options
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download [ Add to Cart ]
10005454 Instruction Manual, Bio-Scale Mini UNOsphere Q & S Cartridges, 1 and 5 ml, Rev C Click to download
5326 Chromatography Column Connection Guide, Rev A Click to download [ Add to Cart ]
2724 UNOsphere Q Anion Exchange Support Product Information Sheet, Rev D Click to download [ Add to Cart ]
2729 UNOsphere Q Support Technical Data, Rev A Click to download [ Add to Cart ]
5735 Purification of a Monoclonal Antibody Using a Combination of UNOsphere Rapid S and UNOsphere Q Media Ion Exchange Chromatography After Protein A Capture, Rev A Click to download [ Add to Cart ]
5543 Effective Cleaning and Sanitizing of Anion Exchange Chromatography Resins, Rev A Click to download
4110109 Instruction Manual, UNOsphere Q and S Ion Exchange Media, Rev C Click to download [ Add to Cart ]
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download [ Add to Cart ]