
A western blot screened with 20 antibody samples using the Mini-PROTEAN II multiscreen apparatus. Human serum was electrophoresed in a mini gel, blotted to nitrocellulose, and probed with various rabbit antibodies to human serum proteins. Bio-Rad's goat anti-rabbit AP conjugate and color development reagents, BCIP and NBT, were used to visualize the positive antigens.