Aurum Affi-Gel Blue Mini Columns and Kit

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Overview

Protein Mini Kit
Aurum Serum Protein Mini Kit
Before After

Effective removal of detergents using the ReadyPrep 2-D cleanup kit leads to improved 2-D SDS-PAGE separation. E. coli extracts were spiked with 1% SDS. Left, untreated sample; right, sample treated with the ReadyPrep kit.

Before After

Removal of albumin and IgG from serum using the Aurum serum protein mini kit. Total protein (1.32 mg) was purified on an Aurum serum protein mmini solumn. Left, untreated serum; right, serum treated with the Aurum kit.

Aurum Affi-Gel Blue mini columns effectively remove up to 90% of albumin from serum or plasma samples by affinity chromatography.Albumin removal reduces protein load by 70–80%, allowing the application of 34 times more serum and significantly improving theresolution of polypeptide spots on 2-D gels. Columns are available individually in packs of 25 or 100, or with buffers as part of the Aurum Affi-Gel Blue mini kit.

Aurum Affi-Gel Blue mini columns:

  • Consist of Micro Bio-Spin columns filled with 100–200 mesh Affi-Gel Blue
  • Use a quick and easy spin-column format
  • Provide eluted proteins ready for IEF analysis in less than 30 minutes
Aurum Affi-Gel Blue Mini Kit

732-6712
Kit for removal of albumin from serum/plasma samples, includes Micro Bio-Spin columns prepacked with Affi-Gel Blue gel for 2 preps

List Price:   $66.00
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Aurum Affi-Gel Blue Mini Columns

732-6708
Pkg of 25, Micro Bio-Spin columns prepacked with Affi-Gel Blue gel, 100–200 mesh, for removal of up to 90% albumin from serum/plasma samples

List Price:   $310.00
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Number Description Options
4110139 Instruction Manual, Aurum Affi-Gel Blue Mini Kits and Columns Sample Preparation, Rev B Click to download