CFT Ceramic Fluoroapatite Support

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Overview

CHT/CFT Bottles

CFT Ceramic Fluoroapatite

Flow Rate on IgG Capture

Flow rate on IgG Capture

Upper panel, binding capacity of CFT Type I (orange) and Type II (blue). Lower panel, effect of flow rate on IgG capture using CFT Type II. Bovine IgG (1 mg/ml) was run on an 11 x 47.6 mm column packed with 4 g CFT until an A280 of 0.80 was reached. The loading buffer was 10 mM sodium phosphate, pH 6.75. Elution was performed using a step gradient from 10 to 400 mM sodium phosphate. The binding capacities were 37.0, 31.2, and 31.0 mg/ml, respectively, at 150, 300, and 450 cm/hr. Chromatography was performed on the BioLogic DuoFlow system.

CFT ceramic fluoroapatite (Ca₁₀(PO₄)₆F₂) is a rigid, spherical macroporous support used in the purification of biologically active compounds. CFT is a composite of fluoroapatite and hydroxyapatite prepared by chemically converting hydroxyapatite nanocrystals to fluoroapatite with a fluorine reagent.

CFT can be used under stringent chromatography conditions to separate acidic proteins requiring buffered conditions as low as pH 5, with minimal effect on the solubility or lifetime of the support.

CFT has high binding capacity and may be used reproducibly over an extended number of chromatography runs. Its increased tensile strength, chemical durability, and density provide excellent throughput and consistent performance for all separations, including biopharmaceutical process-scale manufacturing.

Features of CFT include:

Acidic protein separation for applications requiring pH as low as 5.0
High-density particles for fast, simple column packing
Sintering at 700°C (Type II) for heavy-duty, durable supports
Rigid particles for fast cleaning and equilibration
Inorganic calcium phosphate for distinct selectivities

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO9001 registered corporation.

Specifications and Characteristics
Functional groups	Ca²⁺, PO₄^3-, F^-
Nominal mean particle size	40 ± 4 µm
Nominal pore diameter range	600–800 Â
Recommended linear flow rate range	50–300 cm/hr
Operating pH range	5–14
Chemical compatibility (>24 hr)	2 N NaOH, 8 M urea, 6 M guanidine HCl
Regeneration	400 mM sodium phosphate, pH 6.8
Sanitation	1–2 N NaOH or KOH
Autoclavibility (121°C, 20 min)	Yes
Packing density (g/ml packed bed)	0.86 g/ml
Dynamic binding capacity	14–21.5 mg lysozyme/g
Maximum operating pressure	55 bar (800 psi)