Profinia Protein Purification System Buffers and Reagents

Individually packaged reagents are also available for use with the Profinia system. While designed specifically to work with the Profinia system, these reagents also have general applicability within all stages of a purification workflow, from sample preparation to protein detection.

Profinia Bottled Buffers
For added convenience, preformulated buffers are available for native IMAC, GST, and desalting purifications.

Profinia Control Lysate
The Profinia control lysate is a lyophilized, dual-tagged 51 kD target protein that is meant to eliminate concerns over variability in purification buffer solutions or in the purification matrix itself. The control lysate facilitates system setup and initial purification and is included in every Profinia native IMAC and GST starter kit.

Urea and Glutathione Reagents
Powdered urea and glutathione reagents are available for use with denaturing IMAC and GST applications, respectively. The urea packs are ideal for solubilizing and denaturing proteins in E. coli extracts. The glutathione pack is essential for eluting GST fusion proteins from immobilized glutathione resins.

His and GST Antibodies
Histidine (His)-tagged and GST-tagged monoclonal antibodies are used to detect target protein expression of overexpressed 6x His and GST fusion proteins. They are supplied at a concentration of 1 mg/ml in phosphate buffered saline, pH 7.4 with 0.05% NaN_3.