Ready Gel TBE-Urea Precast Gels

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Overview

Migration Pattern for Ready Gel TBE-Urea Gels
Migration Pattern for Ready Gel® TBE-Urea Gels

The banding patterns indicate the optimal separation range (by bp) for each acrylamide percentage in combinations with the specified buffer system. Migrations are based on the leading dye front being run to the bottom of a gel.

Mini Format Precast Gel Selection Guide
Mini-Format Precast Gel Selection Guide
Refer to the following table to select the appropriate gel type and buffers for your polyacrylamide gel-based applications.
 
Gel Type


Application



Sample Buffer Running Buffer
Tris/Glycine


SDS-PAGE
Native PAGE



Laemmli
Native		 Tris/glycine/SDS
Tris/glycine
Tris-Tricine


SDS-PAGE



Laemmli Tris/Tricine/SDS
IEF


IEF



IEF Anode and cathode buffer
Zymogram


Protease detection



Zymogram Tris/glycine/SDS


TBE-urea gels maintain denaturing conditions for analysis of single-stranded DNA and RNA. Nucleic acids between 60 and 200 bases are resolved as sharp, distinct bands. Applications include oligonucleotide purity analysis, RNase protection assays, and northern blotting.

Ready Gel® precast gels are designed for the traditional 8 x 10 cm mini vertical format. Proteins and nucleic acids can be separated by Ready Gel precast gels using the Mini-PROTEAN® Tetra electrophoresis cell (1–4 gels) or, for high-throughput applications, the Mini-PROTEAN® 3 Dodeca™ cell system (1–12 gels).

Gel dimensions (W x L x thickness) 8.6 x 6.8 x 0.1 cm
Cassette dimensions (W x L x thickness) 10 x 8 x 0.4 cm
Gel storage conditions Store flat at 4°c; do not freeze
Gel shelf life* 8–12 weeks for Tris-HCI, Tris-Tricine, zymogram, TBE, TBE-urea
* From date of manufacture.  
Ready Gel TBE-Urea Gel

161-1115
Pkg of 1, 5% precast polyacrylamide gel, 10-well, 30 µl, 8.6 x 6.8 cm (W x L), for use with Mini-PROTEAN electrophoresis cells

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Ready Gel TBE-Urea Gel

161-1133
Pkg of 1, 5% precast polyacrylamide gel, 15-well, 15 µl, 8.6 x 6.8 cm (W x L), for use with Mini-PROTEAN electrophoresis cells

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Number Description Options
6052 Ready Gel to Mini-PROTEAN TBE, TBE-Urea, and Tris-Tricine Precast Gels Catalog Number Conversion Chart, Rev B Click to download [ Add to Cart ]
5535 Mini-PROTEAN Electrophoresis System Brochure, Rev E Click to download [ Add to Cart ]
2364 Ready Gel System Resource Guide Addendum, Rev A Click to download
2144 Ready Gel System Resource Guide (Note: large PDF file - 3.8 MB) Click to download [ Add to Cart ]
LIT188 Instruction Manual, Ready Gel Precast Gels Application Guide, Rev H Click to download